花椒麻味物质的分离纯化和高效液相色谱法的测定Polyunsaturated Fatty Acid Amides Isolated from Zanthoxylum schinifolium and Detected by HPLC
芮光伟,曹蕊,蒋珍菊
摘要(Abstract):
通过对花椒中麻味物质的分离纯化,将纯化产物作为标准品,建立高效液相色谱(HPLC)的方法定量检测花椒中麻味物质含量。通过HPLC进行检测,花椒麻味物质在浓度范围为1.0~14μg/mL内线性关系良好,回收率在99%~102%,RSD<5%。
关键词(KeyWords): 花椒;麻味物质;多烯酰胺;纯化;高效液相色谱
基金项目(Foundation): 四川省食品生物技术省级重点实验室、四川省科技厅资助项目(2006J13-054)
作者(Author): 芮光伟,曹蕊,蒋珍菊
参考文献(References):
- [1]张雪松,朱媛.乙醇法提取花椒油树脂的研究[J].食品与生物技术学报,2008,27(2):75-77.
- [2]陈立华,谢兰,谢晶曦.化学合成法确证柄果花椒酰胺的结构[J].药学学报,1990,25(12)9:26-928.
- [3]张灿奎,郑庆安,糜留西,等.刺壳椒化学成分研究[J].武汉植物学研究,2000,18(5):441-442.
- [4]任丽娟,谢凤指,冯菊仙,等.柄果花椒树皮的化学成分研究[J].药学学报,1984,19(4):268-273.
- [5]王振忠,武文洁.花椒麻味素的研究概况[J].食品与药品,2006,8(3):26-29.
- [6]Xiaogen Yang.Aroma Constituents and Alkylamides of Red and Green Huajiao(Zanthoxylum bungeanum and Zanthoxylum schinifolium)[J].Journal of Agricultural and Food Chemistry,2008,56:1689-1696.
- [7]刘雄,阚建全,付陈梅,等.花椒麻味成分的提取与分离技术[J].食品与发酵工业,2004,30(9):112-116.
- [8]Michael Bandell,Gina M Story,Sun Wook Hwang,et al.Noxious Cold Ion Channel TRPA1Report Is Activated by Pungent Compounds and Bradykinin[J].Neuron,2004,41:849-857.
- [9]曹蕊,杨潇,蒋珍菊,等.红花椒油和青花椒油中脂肪酸组成的GC-MS的对比研究[J].中国调味品,2011(8):102-105.
- [10]杨潇,芮光伟,钟智超,等.9种花椒超临界CO2萃取物中化学成分的GC/MS/AMDI分析[J].食品与发酵工业,2011,37(3):158-162.
- [11]Koo J.Hydroxy-A-sanshool activates TRPV1and TRPA1in sensory neurons[J].European Journal of Neuroscience,2007,26(5):1139-1147.
- [12]Lennertz R,Tsunozak iM,Bautista D,et al.Physiological Basis of Tingling Paresthesia Evoked by Hydroxy-alphaSanshool[J].Journal of Neuroscience,2010,30(12):4353-4361.
- [13]Okumura Y.Activation of TRPV1and TRPA1by Black Pepper Components[J].Bioscience,Biotechnology and Biochemistry,2010,74(5):1068-1072.
- [14]Riera C.Compounds from Sichuan and Melegueta peppers activate,covalently and non-covalently,TRPA1and TRPV1channels[J].British Journal of Pharmacology,2009,157(8):1398-1409.
- [15]莫彬彬,连宾,万固存,等.超临界CO2分步萃取花椒香气和麻味物质的初步研究[J].食品科学,2009,30(8):201-203.
- [16]Chen I,Chen T,Lin W,et al.Isobuty lamides from the fruit of Zanthoxylum integrifoliolum[J].Phytochemistry,1999,52(2):357-360.
- [17]Jang K.New polyunsaturated fatty acidamides isolated from the seeds o f Zanthoxylum piperitum[J].Archives of Pharmacal Research,2008,31(5):569-572.
- [18]Kash iw ada Y.Amides of the fruit of Zanthoxylum spp[J].Phytochemistry,1997,44(6):1125-1127.
- [19]Rout P,Na ik S,Rao Y,et al.Extraction and composition o f volatiles from Zanthoxylum rhesta:Comparison of subcritical CO2and traditional processes[J].The Journal of Supercritical Fluids,2007,42(3):334-341.
- [20]Xiong Q,Shi D,Yamamoto H,et al.Alky lamides from pericarps of Zanthoxylum bungeanum[J].Phytochemistry,1997,46(6):1123-1126.
- [21]Etsuko Sugai,Yasujiro Morimitsu,Yusaku Iwasaki,et al.Pungent Qualities of Sanshool-Related Compounds Evaluated by a Sensory Test and Activation of Rat TRPV1[J].Biosici.Bitechnol.Biochem.,2005,69(10):1951-1957.