杨成凤,郑凌君,廖尊胜,刘晓艳,吕旭聪,林占熺,黄一帆,赵超,刘斌

• 1:福建农林大学食品科学学院
• 2:国家菌草工程技术研究中心

摘要(Abstract)：

对桑黄固体和液体发酵培养条件及菌丝多糖的提取工艺进行优化。结果表明:桑黄液体发酵培养基的最佳碳源是玉米粉和黄豆粉,最佳氮源是酵母浸出汁和黄豆粉,最佳碳氮比25∶1,温度28℃,装液量80 m L,转速110 r/min,发酵培养时间9天。以巨菌草为主要基料进行固体发酵培养,桑黄菌丝长势良好。巨菌草发酵培养的桑黄菌丝多糖的最佳提取工艺为温度90℃,料液比1∶40,提取时间1.5 h,以3倍体积的乙醇醇沉多糖的条件下多糖得率最高,达到12.06%,高于其他栽培方式的桑黄菌丝体多糖含量。

关键词(KeyWords)： 桑黄;巨菌草;液体发酵;菌丝多糖;提取

Abstract:

Keywords:

基金项目(Foundation): 国家菌草工程技术研究中心攻关课题(JCGG1406)及开放课题(JCJJ13012);; 福建省教育厅项目(JB13051);; 福建省高等学校科技创新团队支持计划[闽教科(2012)03号];; 福建农林大学科技创新团队资助项目(cxtd12009)

作者(Author): 杨成凤,郑凌君,廖尊胜,刘晓艳,吕旭聪,林占熺,黄一帆,赵超,刘斌

参考文献(References)：

• [1]李林玉,金航,张金渝,等.中国药用真菌[J].微生物学杂志,2007(2):57-61.
• [2]Guo X,Zou X,Sun M.Optimization of extraction process by response surface methodology and preliminary characterization of polysaccharides from Phellinus igniarius[J].Carbohydrate Polymers,2010,80(2):344-349.
• [3]Wang Y,Yu J,Zhang C,et al.Influence of flavonoids from Phellinus igniarius on sturgeon caviar:antioxidant effects and sensory characteristics[J].Food Chemistry,2012,131(1):206-210.
• [4]Lung M Y,Tsai J C,Huang P C.Antioxidant properties of edible basidiomycete Phellinus igniarius in submerged cultures[J].J Food Sci.,2010,75(1):18-24.
• [5]Zhu T,Kim S H,Chen C Y.A medicinal mushroom:Phellinus linteus[J].Curr.Med.Chem.,2008,15(13):1330-1335.
• [6]IfákováL,Baldrian P.Fungal polysaccharide monoxygenases:new players in the decomposition of cellulose[J].Fungal Ecology,2012(5):481-489.
• [7]李瑞雪,汪泰初,贾鸿英,等.液体发酵技术培养桑黄的工艺条件优化试验[J].中国蚕业,2010(3):20-24.
• [8]Lee C,Lee S,Cho K J,et al.Mycelial cultivation of Phellinus linteus using cheese-processing waste and optimization of bioconversion conditions[J].Biodegradation,2011,22(1):103-110.
• [9]Wu M,Jiang D,Liu T,et al.Structural analysis of Water-soluble polysaccharide PIP1 extracted from the cultured mycelium of Phellinus igniarius[J].Chemical Research in Chinese Universities,2006,22(6):708-711.
• [10]Dai Y C,Zhou L W,Cui B K,et al.Current advances in Phellinus sensu lato:medicinal species,functions,metabolites and mechanisms[J].Appl Microbiol Biotechnol,2010,87(5):1587-1593.
• [11]王凯,林占熺,曹剑虹.菌草栽培的9种灵芝菌株生长状况和多糖含量比较[J].安徽农业科学,2012,40(21):10849-10852.
• [12]Schmitt C,Turgeon S L.Protein/polysaccharide complexes and coacervates in food systems[J].Adv Colloid Interface Sci.,2011,167(1-2):63-70.