基于高通量测序揭示臭咸鸡蛋及其发酵液微生物多样性Revealing Microbial Diversity of Stinky and Salted Eggs and Their Fermentation Broth Based on High-Throughput Sequencing
袁宇涵,王岩,金泽林,张亮,王亮,刘云国
摘要(Abstract):
复杂的微生物群落在臭咸鸡蛋的发酵过程中起着重要作用。为研究臭咸鸡蛋与发酵液中微生物群落的关系,采用高通量测序技术分析了细菌多样性与真菌多样性。结果表明,臭咸鸡蛋中盐厌氧菌属是优势细菌属,帚枝霉属为优势真菌属,发酵液中微生物群落丰富度与均匀度高于臭咸鸡蛋,优势细菌属为盐单胞菌属,初步判定这些微生物很可能是臭咸鸡蛋发酵的核心微生物。该研究揭示了发酵臭咸鸡蛋与其发酵液的微生物群落结构多样性,为筛选及利用其特色微生物提供了理论依据。
关键词(KeyWords): 微生物群落;臭咸鸡蛋;发酵液;高通量测序
基金项目(Foundation): 山东省重点研发计划(医用食品专项计划)(2019YYSP026);; 临沂市重点研发计划(2020ZX028)
作者(Author): 袁宇涵,王岩,金泽林,张亮,王亮,刘云国
参考文献(References):
- [1]邓高文,蒋立文,刘洋.基于Illumina MiSeq高通量测序技术分析5种不同臭豆腐生坯细菌多样性[J].中国酿造,2021,40(9):155-158.
- [2]HAN D M,CHUN B H,KIM H M,et al.Characterization and correlation of microbial communities and metabolite and volatile compounds in doenjang fermentation[J].Food Research International,2021,148:110645.
- [3]张雅卿,叶书建,周睿,等.发酵食品风味物质及其相关微生物[J].酿酒科技,2021(2):85-96.
- [4]陈倩,李永杰,扈莹莹,等.传统发酵食品中微生物多样性与风味形成之间关系及机制的研究进展[J].食品工业科技,2021,42(9):412-419.
- [5]WANG X D,BAN S D,HU B D,et al.Bacterial diversity of Moutai-flavour Daqu based on high-throughput sequencing method[J].Journal of the Institute of Brewing,2017,123(1):138-143.
- [6]ZHAO D D,HU J,CHEN W X.Analysis of the relationship between microorganisms and flavour development in dry-cured grass carp by high-throughput sequencing,volatile flavour analysis and metabolomics[J].Food Chemistry,2022,368:130889.
- [7]HU Y Y,WANG H,KONG B H,et al.The succession and correlation of the bacterial community and flavour characteristics of Harbin dry sausages during fermentation[J].LWT-Food Science and Technology,2021,138:110689.
- [8]周迎芹,杨明柳,殷俊峰,等.清酒乳杆菌对臭鳜鱼食用品质及挥发性风味物质的影响[J].中国食品学报,2021,21(9):160-168.
- [9]BALDRAIN P,VETROVSKY T,LEPINAY C,et al.High-throughput sequencing view on the magnitude of global fungal diversity[J].Fungal Diversity,2022,114:539-547.
- [10]SPERANSKAYA A S,KRINITSINA A A,SHIPULIN G A,et al.High-throughput sequencing for the authentication of food products:problems and perspectives[J].Russian Journal of Genetics,2018,54(9):1003-1012.
- [11]MOUNE S,MANAC'H N,HIRSCHLER A,et al.Haloanaerobacter salinarius sp.nov.,a novel halophilic fermentative bacterium that reduces glycine-betaine to trimethylamine with hydrogen or serine as electron donors;emendation of the genus Haloanaerobacter[J].International Journal of Systematic Bacteriology,1999,49:103-112.
- [12]DU F M,ZHANG X Y,GU H R,et al.Dynamic changes in the bacterial community during the fermentation of traditional Chinese fish sauce (TCFS) and their correlation with TCFS quality[J].Microorganisms,2019,7(9):371.
- [13]徐琼,刘洋,曲勤凤,等.高通量测序分析不同地区红腐乳细菌多样性[J].食品科学,2020,41(10):110-116.
- [14]YAO Y P,ZHOU X Y,HADIATULLAH H,et al.Determination of microbial diversities and aroma characteristics of Beitang shrimp paste[J].Food Chemistry,2021,344:128695.
- [15]WOODS D F,KOZAK I M,FLYNN S,et al.The microbiome of an active meat curing brine[J].Frontiers in Microbiology,2019,9:3346.
- [16]MWIRICHIA R,MUIGAI A W,TINDALL B,et al.Isolation and characterisation of bacteria from the haloalkaline Lake Elmenteita,Kenya[J].Extremophiles,2010,14(4):339-348.
- [17]FRAZILIO D A,DE ALMEIDA O G G,DE OLIVEIRA C A F,et al.Metataxonomics contributes to unravel the microbiota of a Brazilian dairy[J].Journal of Dairy Research,2020,87(3):360-363.
- [18]MA G,郭奕芳.肠杆菌属:一种日受重视的医院内感染病原菌[J].国外医学(微生物学分册),1989(2):77-79.
- [19]张艳,李秋芬,成钰,等.水体中盐单胞菌属(Halomonas)细菌实时荧光定量PCR检测方法的建立及应用[J].应用与环境生物学报,2016,22(1):140-145.
- [20]LEE J C,KIM S J,WHANG K S.Halomonas sediminicola sp nov.,a moderately halophilic bacterium isolated from a solar saltern sediment[J].International Journal of Systematic and Evolutionary Microbiology,2016,66:3865-3872.
- [21]BARBOSA S G,PEIXOTO L,TER H A,et al.Investigating bacterial community changes and organic substrate degradation in microbial fuel cells operating on real human urine[J].Environmental Science-Water Research & Technology,2017,3(5):897-904.
- [22]CORTES M V D B,GUIMARAES R A,FREIRE D M G,et al.An overview of the virulence factors and the biocontrol potential of Sarocladium oryzae[J].Fungal Biology Reviews,2021,37:1-7.
- [23]KEMP N D,VAUGHAN M M,MCCORMICK S P,et al.Sarocladium zeae is a systemic endophyte of wheat and an effective biocontrol agent against Fusarium head blight[J].Biological Control,2020,149:104329.