孙艺涵,刘晓茂,黄学者,周政,齐双,贾光群,徐向东,崔宗岩

• 1:河北医科大学公共卫生学院
• 2:河北省环境与人群健康重点实验室
• 3:秦皇岛海关技术中心

摘要(Abstract)：

为了解决蜂蜜新型掺假问题，该研究建立了一种测定蜂蜜中外源淀粉酶的离心超滤-高效液相色谱(high performance liquid chromatography, HPLC)法，并将其应用于蜂蜜的掺假鉴别。蜂蜜样品经过离心超滤，提取净化酶蛋白，采用Bio-Bond C8反相色谱柱(250 mm×4.6 mm, 3μm)进行分离，以95%乙腈(含0.02%三氟乙酸)和5%乙腈-水溶液(含0.02%三氟乙酸)为流动相进行梯度洗脱，采用紫外检测器检测。通过对前处理和色谱条件进行优化，实现了蜂蜜中外源淀粉酶的准确定性和定量检测。结果表明，蜂蜜外源淀粉酶在20～1 000μg/mL的浓度范围内呈良好线性(R~2=0.999 3),该方法的定量限为10 mg/kg,在10,25,50,100 mg/kg 4个添加浓度下，外源淀粉酶的平均回收率为87.2%～94.1%,相对标准偏差为3.7%～7.9%。将该方法应用于32批蜂蜜样品的检测，外源淀粉酶的检出率为31.25%,与非变性聚丙烯酰胺凝胶电泳(native polyacrylamide gel electrophoresis, Native-PAGE)法结果一致。该研究方法操作简便，结果准确可靠，适用于蜂蜜中外源淀粉酶的定量检测，为蜂蜜的掺假鉴别提供了新的技术支撑。

关键词(KeyWords)： 蜂蜜;外源淀粉酶;离心超滤;高效液相色谱法;掺假鉴别

Abstract:

Keywords:

基金项目(Foundation): 海关总署科研项目(2024HK106)

作者(Author): 孙艺涵,刘晓茂,黄学者,周政,齐双,贾光群,徐向东,崔宗岩

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