王玉梅,李洋,罗佳沂,栗晓静,李文,毛瑞丰

• 1:广西大学轻工与食品工程学院
• 2:广西民族大学化学化工学院广西林产化学与工程重点实验室

摘要(Abstract)：

使用孔径为0.05μm的陶瓷膜，在TMP=0.35 MPa、TEM=35℃、CFV=4～5 m/s的条件下过滤泡菜汁，通过膜通量的变化、微生物的截留率、污染膜上微生物的分离鉴定、污染膜形貌观察、污染膜官能团分析、EPS含量的变化等分析了膜生物污染的情况。结果显示：随着膜过滤泡菜汁时间的延长，膜通量逐渐下降，运行至15 h时膜通量降低了43.49%。膜过滤运行3,6,9,12,15 h与运行1 h相比较，泡菜汁中细菌的截留率分别降低为0.0008%、0.01%、0.017%、0.03%、0.04%;乳酸菌的截留率分别降低为0.010%、0.012%、0.018%、0.027%、0.04%;真菌的截留率分别降低为0.005%、0.007%、0.011%、0.013%、0.0133%。从污染的膜中共分离出细菌和真菌分别为16株和5株，细菌主要为芽孢杆菌(Bacillus)、放线菌(Actinobacteria bacterium)、发酵黏液乳杆菌(Limosilactobacillus fermentum)及乳酸乳杆菌(Lactobacillus lactis);真菌主要为卡尼克酵母菌(Sporobolomyces carnicolor)和库德里阿兹威毕赤酵母菌(Pichia kudriavzevii)。膜过滤泡菜汁运行3 h后的污染膜孔径内表面覆盖着少量的污染物，膜面孔隙中夹杂有卵球状、杆状生物细胞，运行时间越长，膜表面的污染物质就越多。引起膜污染的物质主要是蛋白质、多糖、泡菜汁中的酚类物质以及少量脂类化合物。经膜过滤后，EPS含量为8.52 mg/mL,运行15 h时EPS含量增加25.15%。最终确定膜过滤泡菜汁周期为15 h,为生物膜污染控制及清洗提供了理论基础。

关键词(KeyWords)： 泡菜汁;膜过滤;微生物;胞外聚合物;傅里叶变换红外光谱;扫描电镜

Abstract:

Keywords:

基金项目(Foundation): 广西重点研发计划(2018AB45017)

作者(Author): 王玉梅,李洋,罗佳沂,栗晓静,李文,毛瑞丰

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