致牛肉酱生膜菌的分离鉴定及其生膜能力验证Isolation and Identification of Biofilm-forming Strains in Beef Sauce and Analysis of Their Spoilage
郭壮,董蕴,尚雪娇,倪慧,周明会,张振东
摘要(Abstract)：
采用传统培养方法,从生膜牛肉酱中分离到了4株酵母菌,经菌落与显微形态观察和26SrDNA中D1/D2区序列分析,发现它们与Candida metapsilosis序列相似度最高,属于Candida parapsilosis complex,经系统发育分析,所分离到的4株酵母菌鉴定为Candida metapsilosis。此外,将这4株酵母菌重新接入到无菌牛肉酱中培养,发现它们能引起牛肉酱产膜,因此确认分离到的4株Candida metapsilosis是牛肉酱生膜的腐败菌。
关键词(KeyWords)： 牛肉酱;生膜菌;假丝酵母属
基金项目(Foundation): 湖北省高等学校优秀中青年科技创新团队计划项目(T201616)
作者(Author): 郭壮,董蕴,尚雪娇,倪慧,周明会,张振东
参考文献(References)：
- [1]饶瑜,常伟,唐洁,等.食品中腐败酵母的研究进展[J].食品与发酵科技,2013(4):61-64.
- [2]胡怀容.腌制大头菜中腐败微生物的调查及控制研究[D].重庆:西南大学,2015.
- [3]朱玲.耐高渗透压腐败酵母的分离与控制研究[D].广州:华南理工大学,2010.
- [4]Dallacosta L M,Morello L G,Conte D,et al.Comparison of DNA extraction methods used to detect bacterial and yeast DNA from spiked whole blood by real-time PCR[J].2017(140):61-66.
- [5]Ramos-Garza J,Bustamante-Brito R,ngeles de Paz G,et al.Isolation and characterization of yeasts associated with plants growing in heavy-metal-and arsenic-contaminated soils[J].Canadian Journal of Microbiology,2016,62(4):307-319.
- [6]Gagnon S,Bourbeau D,Levesque R C,et al.Secondary structures and features of the 18S,5.8Sand 26Sribosomal RNAs from the Apicomplexan parasite Toxoplasma gondii[J].Gene,1996,173(2):129-135.
- [7]Wang Q M,Groenewald M,Takashima M,et al.Phylogeny of yeasts and related filamentous fungi within Pucciniomycotina determined from multigene sequence analyses[J].Studies in Mycology,2015(81):27-53.
- [8]Brazee N J,Hulvey J P,Wick R L.Evaluation of partial tef1,rpb2,and nLSU sequences for identification of isolates representing Armillaria calvescens and Armillaria gallicafrom northeastern North America[J]. Fungal Biology,2011,115(8):41-49.
- [9]Wu Y M,Huang P Y,Lu J J,et al.Risk factors and outcomes of candidemia caused by Candida parapsilosis complex in a medical center in northern Taiwan[J].Diagnostic Microbiology&Infectious Disease,2017,90(1):44-49.
- [10]Tavanti A,Davidson A D,Gow N A,et al.Candida orthopsilosis and Candida metapsilosis spp.nov.to replace Candida parapsilosis groups II and III[J].Journal of Clinical Microbiology,2005,43(1):284-292.
- [11]Gácser A,Schfer W,Nosanchuk J S,et al.Virulence of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis in reconstituted human tissue models[J].Fungal Genetics&Biology:FG&B,2007,44(12):1336-1341.
- [12]李亚莉,秘鸣,魏珍珍,等.近平滑假丝酵母(Candida parapsilosis)发酵普洱茶初探[J].茶叶科学,2013(5):436-442.
- [13]任艳秋.近平滑假丝酵母生物立体选择性转化制备(S)-苯基乙二醇的研究[D].无锡:江南大学,2005.