等离子诱变选育木糖醇高产菌及发酵条件优化Mutation Breeding of High-yield Xylitol Strain by Plasma and Optimization of Fermentation Conditions
周龙霞,王燕,郭玉蓉,张海涛,孙自顶,王志超
摘要(Abstract)：
以热带假丝酵母Y-14(Tropical candida)为出发菌株,采用等离子体对其进行诱变,得到最佳诱变条件:在功率120 W下处理150s,致死率达到96%。诱变后经过筛选得到1株高产木糖醇的菌株Y-117,与出发菌株相比,其木糖醇产量提高了30.4%。在单因素试验的基础上,通过响应面分析试验对菌株Y-117的发酵条件进行优化,得到最佳条件:初始木糖添加量105g/L、温度30.5℃、摇床转速190r/min。在此条件下,Y-117的木糖醇产量为76.45g/L,比出发菌株高42%。
关键词(KeyWords)： 木糖醇;热带假丝酵母;等离子诱变;响应面法
基金项目(Foundation): 农业部现代农业生产技术体系建设项目(CARS-28)
作者(Author): 周龙霞,王燕,郭玉蓉,张海涛,孙自顶,王志超
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