海南黑、白胡椒有效成分的检测及其分析Detection and Analysis of Effective Components in Hainan Black and White Pepper
陈晓龙,陈光静,柳中,阚建全
摘要(Abstract)：
文章对海南黑、白胡椒的基本成分进行测定分析,并在此基础上采用固相微萃取法(SPME)对挥发油中香气物质进行提取,经气相色谱-质谱联用仪(GC-MS)对其香气成分进行分析、鉴定,为海南黑胡椒、白胡椒的进一步加工提供实验数据。结果发现海南白胡椒的水分、胡椒碱含量高于黑胡椒,但其总灰分、酸不溶性灰分、水不溶性灰分、醇溶抽提物、不挥发性乙醚抽提物、冷水可溶性抽提物、胡椒油树脂低于黑胡椒。白胡椒的香气成分主要是石竹烯(44.41%)、4-次乙基-4-甲基-3-(1-甲基乙烯基)-1-(1-甲基乙基)-(三重反式)-环己烯(9.37%)、异兰烯(6.60%)、D-柠檬烯(5.50%)等59种化合物,占整个峰面积的90.20%,黑胡椒的香气成分主要是D-柠檬烯(18.04%)、3-蒈烯(13.82%)、石竹烯(12.98%)、6,6-二甲基-2-亚甲基-双环[3.1.1]庚烷(10.73%)等36种化合物,占整个峰面积的93.07%。
关键词(KeyWords)： 胡椒;固相微萃取法;气相色谱-质谱联用法(GC-MS)
基金项目(Foundation):
作者(Author): 陈晓龙,陈光静,柳中,阚建全
参考文献(References)：
- [1]柳中,阚建全,李银聪,等.不同方法提取的海南黑、白胡椒香气物质GC-MS比较分析[J].食品工业科技,2012(2):175-179,198.
- [2]邬华松,杨建峰,林丽云.中国胡椒研究综述[J].中国农业科学,2009(7):2469-2480.
- [3]包兰兰,金桩.胡椒碱降血脂作用的实验研究[J].中国民族医药杂志,2004(1):22-23.
- [4]Vijayakumar R S,Surya D,Nalini N.Antioxidant efficacy of black pepper(Piper nigrum L.)and piperine in rats with high fat diet induced oxidative stress[J].Redox Rep,2004,9(2):105.
- [5]Mittal R,Gupta R L.In vitro antioxidant activity of piperine[J].Methods Find Exp Clin Pharmacol,2000,22(5):271.
- [6]Selvendiran K,Banu S M,Sakthisekaran D,et al.Protective effect of piperine on benzo(a)pyrene-induced lung carcinogenesis in Swiss albino mice[J].Clinica Chimica Acta,2004,350(1-2):73.
- [7]Pradeep C R,Kuttan G.Effect of piperine on the inhibition of lung metastasis induced B16F-10 melanoma cells in mice[J].Clin Exp Metastasis,2002,19(8):703.
- [8]Sunila E S,Kuttan G.Immunomodulatory and antitumor activity of Piper longum Linn and piperine[J].Journal of Ethnopharmacology,2004,90(223):339.
- [9]Lee S A,Hong S S,Han X H,et al.Piperine from the fruits of Piper longum with inhibitory effect on monoamine oxidase and antide-pressant like activity[J].Chemical Pharm Bull(Tokyo),2005,53(7):832.
- [10]Kong L D,Cheng C H,Tan R X.Inhibition of MAO A and B by some plant-derived alkaloids,phenols and anthraquinones[J].J Ethnopharmacol,2004,91(2-3):351.
- [11]Pathak N,Khandelwal S.Modulation of cadmium induced alterations in murine thymocytes by piperine:oxidative stress,apoptosis,phenotyping and blastogenesis[J].Biochemical Pharmacol,2006,72(4):486.
- [12]Larson R A.The antioxidants of higher plants[J].Phyto Chemicalistry,1988(4):969-978.
- [13]刘红星,陈少东,陈福北,等.索氏法提取干姜挥发油及其化学成分研究[J].中国调味品,2011,35(11):105-106.
- [14]买买提江·依米提,米红字,艾合买提·沙塔尔,等.索氏提取法精炼及杏仁油提取工艺的研究[J].新疆化工,2009(3):11-14.
- [15]包振伟,顾林,白东辉,等.响应面法优化黑胡椒油树脂提取工艺[J].食品科学,2013(14):17-21.
- [16]李祖光,乔剑峰,胡伟,等.固相微萃取-气相色谱/质谱法分析白胡椒粉的风味成分[J].理化检验(化学分册),2005(11):33-35.
- [17]郑品梅,邹纲明,李彦威,等.胡椒油的研究进展[J].食品科技,2007(1):25-28.
- [18]陈文学,豆海港,仇厚援,等.胡椒抗氧化产物提取条件的研究[J].热带农业科学,2005,25(2):1-3.
- [19]Pino J,Rodriguez Feo G,Borges P,et al.Chemicali and sensory properties of black pepper oil[J].Die Nahrung,1990(34):45-48.