响应面法优化鲁氏酵母菌与植物乳杆菌复配培养条件Optimization of Cultivation Conditions of Zygosaccharomyces rouxii and L. plantarum by Response Surface Methodology
赵家圆,高绍金,李艳青,韩齐
摘要(Abstract):
试验探索了鲁氏酵母菌与植物乳杆菌的复配培养条件,在单因素试验基础上进行响应面中的Box-Behnken试验设计,以pH和菌落总数为评价指标,优化用于发酵肉制品生产发酵剂的制备条件。结果表明,两组试验的预测模型均极显著,培养温度、培养时间和复配比例间两两交互作用对发酵液pH值和菌落总数影响显著。最终以菌落总数为响应值优化复配培养条件为培养温度20.71℃,培养时间22.99h,Z.rouxii和L.plantarum的复配比例为1.32。对优化结果进行了验证,实际结果与预测值拟合度达94.12%。
关键词(KeyWords): 植物乳杆菌;鲁氏酵母菌;响应面优化;复配培养
基金项目(Foundation): 黑龙江八一农垦大学引进人才科研启动计划资助项目(XYB201803);; 大庆市指导性科技计划项目(zd-2019-35)
作者(Author): 赵家圆,高绍金,李艳青,韩齐
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