邵宏宏,相兴伟,王琦,周向阳,周秀锦,沈飚,傅谧妮

• 1:舟山出入境检验检疫局
• 2:浙江省海洋开发研究院

摘要(Abstract)：

建立了液相色谱串联质谱法(HPLC-MS/MS)测定鱼酱中辐照相关的多种氨基酸及同分异构体的分析方法,并建立了辐照鱼酱检测方法。样品通过盐酸水解,采用Waters Sunfire C18柱,0.1%甲酸溶液和甲醇为流动相进行梯度洗脱,正离子(ESI+)模式可快速有效分离及准确测定辐照鱼酱中的对酪氨酸(p-Tyrosine)、苯丙氨酸(Phenylalanine)及其辐照特异性产物间酪氨酸(m-Tyrosine)和邻酪氨酸(o-Tyrosine)的含量。在10~500μg/L范围内,所测氨基酸及同分异构体的线性相关系数为0.9985~0.9998,回收率为75.1%~104.5%,相对标准偏差小于8.9%。方法适用于对含蛋白质鱼酱中辐照相关的多种氨基酸和其同分异构体的测定,从而对其是否经过辐照进行准确鉴定。

关键词(KeyWords)： 辐照;鱼酱;氨基酸;同分异构体;液相色谱串联质谱

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金项目(LQ15c200005);; 浙江省公益技术研究农业项目(2015C32101);; 浙江省科技计划项目(2016C37041)

作者(Author): 邵宏宏,相兴伟,王琦,周向阳,周秀锦,沈飚,傅谧妮

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