鲁佩玉,孙青华

• 1:青岛科技大学化工学院

摘要(Abstract)：

氨基酸是极具应用前景的生物化学品,市场容量要求不断增加。它们的适用范围广泛,包括动物饲料添加剂、增味剂、化妆品成分、制药和医疗领域的营养素等。由于基因工程技术和生物工程技术的进步,特别是借助于工程菌的发酵生产技术,在氨基酸工业生产中发挥着重要作用。尽管发酵法有众多优点,但仍需进一步改进,从而提高产率,降低生产成本。文章阐述了发酵工艺对工业氨基酸生产的重要性,介绍了发酵工艺设计并提出了工艺优化方法,为氨基酸工业的进一步发展提供了技术支撑。

关键词(KeyWords)： 氨基酸;发酵;过程优化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(21576145)

作者(Author): 鲁佩玉,孙青华

参考文献(References)：

• [1]Cesari M,Rossi G P,Sticchi D,et al.Is homocysteine important as risk factor for coronary heart disease[J].Nutr.Metab.Cardiovasc.Dis.,2005,15(2):140-147.
• [2]Paul B D,Sbodio J I,Xu R,et al.Cystathionineγ-lyase deficiency mediates neurodegeneration in Huntington's disease[J].Nature,2014,509:96-100.
• [3]Marz U.World markets for fermentation ingredients[EB/OL].Available from:http://www.bccresearch.com/marketresearch/food-and-beverage/fermentation ingredients-fod020c.html,2009-02-17.
• [4]Ugimoto M S.Amino Acids,Production Processes[M].Hoboken:John Wiley&Sons,2010.
• [5]Ivanov K,Stoimenova A,Obreshkova D,et al.Biotechnology in the production of pharmaceutical industry ingredients:amino acids[J].Biotechnol.Biotechnol.Equip.,2014,27(2):3620-3626.
• [6]Scheper H,Lammers F.Fermentation monitoring and process control[J].Curr.Opin.Biotechnol.,1994(2):187-191.
• [7]Villadsen J,Lidén G,Nielsen J.Bioreaction Engineering Principles[M].New York:Springer,2011.
• [8]Delaunay S,Lapujade P,Engasser J M,et al.Flexibility of the metabolism of Corynebacterium glutamicum 2262,a glutamic acid-producing bacterium,in response to temperature upshocks[J].J.Ind.Microbiol.Biotechnol.,2002,28:333-337.
• [9]Brautaset T,JakobsenM,Josefsen K D,et al.Bacillus methanolicus:a candidate for industrial production of amino acids from methanol at 50℃[J].Appl.Microbiol.Biotechnol.,2007,74:22-34.
• [10]Aboutaleb K A.Enhancing production of amino acids from Bacillus spp.using batch and fed-batch fermentation strategies[J].Br.Microbiol.Res.J.,2015(5):257-272.
• [11]Hermann T.Industrial production of amino acids by coryneform bacteria[J].J.Biotechnol.,2003,104:155-172.
• [12]Koyoma Y,Ishii T,Kawahara Y,et al.Method for producing L-glutamic acid by continuous fermentation[P].EP844308,1998.
• [13]Kumar R,Vikramachakravarthi D,Pal P.Production and purification of glutamic acid:a critical review towards process intensification[J].Chem.Eng.Process,2014,81:59-71.
• [14]M9ntt9ri M,Van Bruggen B,Nystr9m M.Separation and Purification Technologies in Biorefineris[M].Hoboken:John Wiley&Sons,2013.
• [15]Lara A R,Galindo E,Ramírez O T,et al.Living with heterogeneitiesin bioreactors:understanding the effects of environmental gradients on cells[J].Mol.Biotechnol.,2006,34:355-381.
• [16]Schmidt F R.Optimization and scale up of industrial fermentation processes[J].Appl.Micorbiol.,Biotchnol.,2005,68:425-435.
• [17]Neubauer P,Junne S.Scale-down simulators for metabolic analysis of large-scale bioprocesses[J].Curr.Opin.Biotechnol.,2010,21(1):114-121.
• [18]Delvigne F,Destain J,Thonart P.A methodology for the design of scale-down bioreactors by the use of mixing and circulation stochastic models[J].Biochem.Eng.J.,2006,28:256-268.
• [19]Elmar H,Arno P B,Charles L C.Development of Sustainable Bioprocesses:Modeling and Assessment[M].Hoboken:John Wiley&Sons,2007.
• [20]Campolo M,Paglianti A,Soldati A.Fluid dynamic efficiency and scale-up of are treated blade impeller CSTR[J].Ind.Eng.Chem.Res.,2002,41:164-172.
• [21]Babi D K,Cruz M S,Gani R.Fundamentals of Process Intensification:A Process Systems Engineering View[M].Berlin:Springer,2016.
• [22]Vaghari H,Eskandari M,Sobhani V,et al.Process intensification for production and recovery of biological products[J].Am.J.Biochem.Biotechnol.,2015,11:37-43.
• [23]Ikeda M,Takeno S.Amino Acid Production by Corynebacterium glutamicum[M].Berlin:Springer,2013:107-147.