纪雅飞,熊琦

• 1:山西大学生命科学学院

摘要(Abstract)：

为提高纳豆芽孢杆菌对无机硒的生物转化率,通过单因素实验和响应面法对纳豆芽孢杆菌富硒的发酵条件进行优化,对样品进行透析和消化处理,并采用流动注射氢化物-火焰原子吸收光谱法测定有机硒含量,获得最佳有机硒转化率。优化后的发酵条件为:添加亚硒酸钠的同时接入菌株,亚硒酸钠质量浓度为10μg/mL,培养基初始pH为7.5,培养时间为10h,接种量为3.5%,最佳转化率达到91.21%。各单因素对有机硒转化率影响的大小为:接种量>培养基初始pH值>培养时间。

关键词(KeyWords)： 响应面法;纳豆芽孢杆菌;亚硒酸钠;富硒;发酵条件

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 纪雅飞,熊琦

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