美拉德热反应条件对大豆酶解液风味物质形成的影响Effect of Maillard Thermal Reaction Conditions on Formation of Flavor Substances of Soybean Enzymatic Hydrolysate
曹振宇,阮秋凤,谭佳,童星,黄强
摘要(Abstract):
美拉德热反应是食物风味生成的重要反应。试验分析了以大豆酶解液制得增香大豆油的挥发性风味物质,得出其关键风味化合物为醛类、酮类、杂环类和含硫化合物等。同时,研究了美拉德热反应pH、反应温度等条件对氨基酸和关键风味物质含量的影响,通过皮尔森相关性分析、聚类分析等方法对关键风味物质的生成机制进行探索,得出美拉德热反应温度的提升促进关键风味物质含量的提升,而pH的提升对风味物质的调节更细微,除吡嗪类化合物整体呈上升趋势外,醛类、含硫化合物均呈现不同程度的上升或下降趋势。酶解液氨基酸对风味物质的影响与风味物质的生成机制有关,对异丁醛、3-甲基丁醛、吡嗪类和含硫化合物的影响较大。该研究进一步揭示了美拉德热反应中风味物质的形成机制,可为后续食品的增香提供理论基础。
关键词(KeyWords): 大豆酶解液;风味;氨基酸;美拉德热反应;增香大豆油
基金项目(Foundation): 广东省调味食品生物发酵先进技术企业重点实验室开放基金项目(2017B030302002)
作者(Author): 曹振宇,阮秋凤,谭佳,童星,黄强
参考文献(References):
- [1]曾庆祝,崔燕玲,张淑宜,等.花生粕水解液及其美拉德产物的风味与氨基酸分析[J].广州大学学报(自然科学版),2018(17):47-53.
- [2]张左勇.基于豆粕美拉德反应的牛肉特征风味形成调控及应用研究[D].合肥:合肥工业大学,2022.
- [3]芦鑫,张丽霞,孙强,等.配料组成对高温芝麻饼粕蛋白酶解物制备肉味香精的影响[J].食品工业科技,2024,45(14):50-61.
- [4]HE S D,ZHANG Z Y,SUN H J,et al.Contributions of temperature and L-cysteine on the physicochemical properties and sensory characteristics of rapeseed flavor enhancer obtained from the rapeseed peptide and D-xylose Maillard reaction system[J].Industrial Crops & Products,2019,128:455-463.
- [5]CAO M L,WATERHOUSE G I N,SUN W Z,et al.Effects of reaction medium type and concentration on the physicochemical properties and flavors of soybean meal hydrolysate-based Maillard reaction products[J].LWT-Food Science and Technology,2023,182(10):114762.
- [6]YU M,HE S D,TANG M M.Antioxidant activity and sensory characteristics of Maillard reaction products derived from different peptide fractions of soybean meal hydrolysate[J].Food Chemistry,2017,243:249-257.
- [7]YU H,ZHANG R Y,YANG F W,et al.Control strategies of pyrazines generation from Maillard reaction[J].Trends in Food Science & Technology,2021,112:795-807.
- [8]李菁,何述栋,霍晓兵,等.基于脱脂芝麻渣酶解液美拉德反应制备浓香型调和芝麻油的研究[J].中国调味品,2024,49(1):89-93.
- [9]胡龙腾.芝麻粕酶解美拉德反应制备风味物及其在低钠调味盐中的应用[D].合肥:合肥工业大学,2022.
- [10]刘翔.山茶粕酶解及其美拉德反应产物风味和安全性研究[D].合肥:合肥工业大学,2021.
- [11]刘春晓,王斌,高盼,等.美拉德生香源反应制备浓香芝麻油工艺优化[J].油脂加工,2022,47(2):28-33.
- [12]KOVáTS E.Gas-chromatographische charakterisierung organischer verbindungen.Teil 1:retentionsindices aliphatischer halogenide,alkohole,aldehyde,and ketone[J].Helvetica Chimica Acta,1958,41:1915-1932.
- [13]阮秋凤.鱼糜及鱼糜制品的特征风味成分与指纹图谱[D].武汉:华中农业大学,2022.
- [14]BURDOCK G A.Fenaroli 's Handbook of Flavor Ingredients[M].Boca Raton:Taylor and Francis Group,2010.
- [15]程建军,王兆华,于殿宇,等.加工方式对大豆油中挥发性物质的影响[J].中国食品学报,2024,24(2):248-259.
- [16]吴思雨,谢萱,刘雨雯,等.基于电子鼻、HS-SPME-GC-MS和HS-GC-IMS评价不同制油工艺对大豆油品质及风味的影响[J].食品科学,2024,45(4):183-196.
- [17]SUN L B,WANG D H,HUANG Z,et al.Structure and flavor characteristics of Maillard reaction products derived from soybean meal hydrolysates-reducing sugars[J].LWT-Food Science and Technology,2024,185:115097.
- [18]WEI C K,NI Z J,THAKUR K,et al.Color and flavor of flaxseed protein hydrolysates Maillard reaction products:effect of cysteine,initial pH,and thermal treatment[J].International Journal of Food Properties,2019,22(1):84-99.
- [19]LOTFY S N,SAAD R,EL-MASSREY K F,et al.Effects of pH on headspace volatiles and properties of Maillard reaction products derived from enzymatically hydrolyzed quinoa protein-xylose model system[J].LWT-Food Science and Technology,2021,145:11328.
- [20]陈臣,周文雅,袁佳杰,等.3-甲基丁醛对奶酪坚果风味的贡献及其生物合成的研究进展[J].食品科学,2020,41(7):228-233.
- [21]KIM J Y,YI B R,LEE C K,et al.Effects of pH on the rates of lipid oxidation in oil-water system[J].Applied Biological Chemistry,2016,59(2):157-161.
- [22]ARSA S,PUECHKAMUTR Y.Pyrazine yield and functional properties of rice bran protein hydrolysate formed by the Maillard reaction at varying pH[J].Journal of Food Science and Technology,2022,59(3):890-897.
- [23]CHEN X,YU J Y,CUI H P,et al.Effect of temperature on flavor compounds and sensory characteristics of Maillard reaction products derived from mushroom hydrolysate[J].Molecules,2018,23(2):247-266.
- [24]ERIC K,RAYMOND L V,ABBAS S,et al.Temperature and cysteine addition effect on formation of sunflower hydrolysate Maillard reaction products and corresponding influence on sensory characteristics assessed by partial least square regression[J].Food Research International,2014,57:242-258.
- [25]LIU J B,LIU M Y,HE C C,et al.Effect of thermal treatment on the flavor generation from Maillard reaction of xylose and chicken peptide[J].LWT-Food Science and Technology,2015,64(1):316-325.
- [26]ANDREA M Y,ENCARNACIóN G,MARIA D G.Processing and Impact on Active Components in Food[M].London:Academic Press,2016:542.
- [27]GAO P,ZHANG W Y,ZHAO X X,et al.The effect of Maillard reaction on flavour development of protein hydrolysates from cheese[J].Food Chemistry,2024,437:137569.
- [28]ZHANG Z Y,ELFALLEH W,HE S D,et al.Heating and cysteine effect on physicochemical and flavor properties of soybean peptide Maillard reaction products[J].International Journal of Biological Macromolecules,2018,120:2137-2146.
- [29]ZHANG Z Y,CHEN J Y,ZHENG L,et al.The potential meat flavoring derived from Maillard reaction products of rice protein isolate hydrolysate-xylose via the regulation of temperature and cysteine[J].Food Chemistry:X,2024,22(2):101491.
- [30]KWAK E J,LIM S I.The effect of sugar,amino acid,metal ion,and NaCl on model Maillard reaction under pH control[J].Amino Acids,2004,27(1):85-90.
- [31]ZAMORA R,HIDALGO F J.Coordinate contribution of lipid oxidation and Maillard reaction to the nonenzymatic food browning[J].Critical Reviews in Food Science and Nutrition,2005,45(1):49-59.
- [32]BUENO M,MARRUFO-CURTIDO A,CARRASCóN V,et al.Formation and accumulation of acetaldehyde and Strecker aldehydes during red wine oxidation[J].Frontiers in Chemistry,2018,6:20.
- [33]ZHANG Z Y,WANG B,CAO Y P.Effect of Maillard reaction products derived from cysteine on the formation of dimethyl disulfide and dimethyl trisulfide during storage[J].Journal of Agricultural and Food Chemistry,2023,71(35):13043-13053.