基于GC-MS的保留指数分析黑胡椒精油挥发性成分Analysis of Volatile Components in Black Pepper Essential Oil Based on GC-MS Retention Index
尹团章,邵佩,吴昭,程书锋
摘要(Abstract):
为分析海南产黑胡椒精油中的挥发性成分,为公众进一步开发利用黑胡椒提供科学依据,采用水蒸气蒸馏法制备了黑胡椒精油,并基于气相色谱-质谱联用技术结合保留指数对精油成分进行了定性分析。从黑胡椒精油中鉴定出了27种主要的挥发性成分,含量较高的组分为β-石竹烯(23.91%)、柠檬烯(17.23%)、3-蒈烯(19.30%)、β-蒎烯(7.83%)和石竹烯氧化物(3.01%)。对δ-杜松烯、柠檬烯、顺式香桧醇(cis-sabinol)、cyclohexene, 3-methyl-6-(1-methylethylidene)-(萜品油烯)、cyclohexene, 1-methyl-4-(1-methylethylidene)-(萜品油烯)、对伞花烃(P-cymene)进行鉴定,使精油的定性结果更准确、可靠。
关键词(KeyWords): 黑胡椒精油;保留指数;挥发性成分;气相色谱-质谱联用技术;水蒸气蒸馏法
基金项目(Foundation): 湖北中烟工业有限责任公司科技项目(2021JSXL3XL2B021)
作者(Author): 尹团章,邵佩,吴昭,程书锋
参考文献(References):
- [1]SALEHI B,ZAKARIA Z,GYAWALI R,et al.Piper species:a comprehensive review on their phytochemistry,biological activities and applications[J].Molecules,2019,24(7):1364.
- [2]于岚,郝正一,胡晓璐,等.胡椒的化学成分与药理作用研究进展[J].中国实验方剂学杂志,2020,26(6):234-242.
- [3]常红,毕耀,王颖,等.黑胡椒精油复合涂层对金华火腿理化特性及食用品质安全的影响[J].食品科学,2020,41(13):193-198.
- [4]ZAHIN M,BOKHARI N,AHMAD I,et al.Antioxidant,antibacterial,and antimutagenic activity of Piper nigrum seeds extracts[J].Saudi Journal of Biological Sciences,2021,28(9):5094-5105.
- [5]徐可文,阚海峰,杜宏金.黑胡椒挥发油的FTIR鉴定与GC-MS分析[J].现代中药研究与实践,2011,25(3):28-30.
- [6]王珏,钟壹鸣,孙也乔,等.3种不同萃取方法对黑胡椒香气物质的气质联用分析[J].热带作物学报,2022,43(5):1055-1063.
- [7]段梦雅,王福清,吴桂苹,等.4种胡椒精油风味特征分析[J].食品科学,2022,43(8):213-219.
- [8]侯冬岩,回瑞华,李铁纯,等.海南黑胡椒果挥发性成分气相色谱-质谱分析[J].质谱学报,2005,26(1):40-42.
- [9]AL-SAYED E,GAD H,KERSH D.Characterization of four Piper essential oils (GC/MS and ATR-IR) coupled to chemometrics and their anti-Helicobacter pylori activity[J].ACS Omega,2021,6(39):25652-25663.
- [10]DOSOKY N,SATYAL P,BARATA L,et al.Volatiles of black pepper fruits (Piper nigrum L.)[J].Molecules,2019,24(23):4244.
- [11]董世良,陈一,刘冰,等.万寿菊油挥发性成分的GC-MS保留指数定性分析[J].食品研究与开发,2018,39(20):152-156.
- [12]叶洵,刘子博,张婷,等.基于GC-MS结合保留指数法建立花椒挥发油指纹图谱[J].中国调味品,2022,47(4):68-73.
- [13]CHAMALI S,BOUAJILA J,DAOUD H,et al.Variation in chemical composition and biological properties of two Tunisian Eucalyptus essential oils under three eco-friendly extraction techniques[J].Journal of Essential Oil Research,2022,34(1):36-53.
- [14]SILVA M,ANDRADE E,ZOGHBI M,et al.The essential oils of Lantana camara L.occurring in North Brazil[J].Flavour and Fragrance Journal,1999,14(4):208-210.
- [15]BARBOSA L,PAULA V,AZEVEDO A S,et al.Essential oil composition from some plant parts of Conyza bonariensis (L.) Cronquist[J].Flavour and Fragrance Journal,2005,20(1):39-41.
- [16]MAIA J,SILVA M,ANDRADE E,et al.Essential oils from Astronium urundeuva (Allemao) Engl.and A.fraxinifolium Schott ex Spreng.[J].Flavour and Fragrance Journal,2002,17(1):72-74.
- [17]FLAMINI G,CIONI P,MORELLI I,et al.Essential oils of the aerial parts of three Salvia species from Jordan:Salvia lanigera,S.spinosa and S.syriaca[J].Food Chemistry,2007,100(2):732-735.
- [18]ADAMS R R.Systematics of Juniperus section Juniperus based on leaf essential oils and random amplified polymorphic DNAs (RAPDs)[J].Biochemical Systematics and Ecology,2000,28(6):515-528.
- [19]TELASCREA M,ARAUJO C,MARQUES M,et al.Essential oil from leaves of Cryptocarya mandioccana Meisner (Lauraceae):composition and intraspecific chemical variability[J].Biochemical Systematics and Ecology,2007,35(4):222-232.
- [20]GOMEZ E,LEDHETTER C,HARTSELL P.Volatile compounds inapricot,plum,and their interspecific hyhrids[J].Journal of Agricultural and Food Chemistry,1993,41(10):1669-1676.
- [21]KABIR Y,AKASAKA-HASHIMOTO Y,KUBOTA K,et al.Volatile compounds of black cumin (Nigella sativa L.) seeds cultivated in Bangladesh and India[J].Heliyon,2020,6(10):5343.
- [22]彭志宏,豆海港.GC-MS分析同时蒸馏-萃取(SDE)黑胡椒挥发性成分[J].中国调味品,2011,36(6):90-92.
- [23]王花俊,齐海英,张峻松.黑胡椒精油挥发性成分分析[J].中国调味品,2017,42(12):138-140.