张欣,赵海磊,郭庆彬,丁文涛,王昌禄

• 1:天津科技大学食品科学与工程学院

摘要(Abstract)：

紫红曲霉(Monascus purpureus)常用于红曲色素发酵及红曲米、红曲酒等食品的生产，但几丁质合成酶对其生长和色素合成的影响尚不清晰。文章构建了几丁质合成酶编码基因ChsⅢ敲除质粒，并利用根癌农杆菌介导转化获得ChsⅢ敲除的紫红曲霉重组菌株ΔChsⅢ。结果表明，ChsⅢ基因敲除对紫红曲霉的生长和繁殖造成一定影响，ΔChsⅢ在PDA和MEA培养基上的菌落直径分别比M9减少10.60%和9.80%,孢子数减少46.67%,液体培养6 d时菌丝干重减少9.22%。敲除ChsⅢ基因后，几丁质含量呈现先减少后增加的趋势，第3天时ΔChsⅢ几丁质含量较M9减少14.92%,而第6天时较M9增加49.48%。敲除ChsⅢ基因使胞外和胞内色素总色价有所降低，但显著提高了黄色素的合成，第9天时胞外、胞内黄色素产量分别较M9提高36.08%和28.20%。RT-qPCR结果表明，敲除ChsⅢ使色素合成相关基因MpPKS5、mppD和mppG表达下调，但显著上调了黄色素合成基因mppE的表达，这可能是ΔChsⅢ黄色素产量和比例提高的原因。文章首次报道了敲除几丁质合成酶基因ChsⅢ对红曲霉生长、几丁质和色素合成的影响，为深入挖掘红曲资源拓宽了思路。

关键词(KeyWords)： 紫红曲霉;几丁质合成酶;红曲色素;基因敲除

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(32272277)

作者(Author): 张欣,赵海磊,郭庆彬,丁文涛,王昌禄

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