郭礼强,崔晓娜,丁葵英,孙朝红,王亮亮,刘春雪

• 1:潍坊出入境检验检疫局
• 2:山东畜牧兽医职业学院

摘要(Abstract)：

建立了芝麻酱中16种真菌毒素(赭曲霉毒素A、T-2毒素、伏马菌素B2、伏马菌素B1、O-甲基杂色曲霉素、蛇形菌素、新茄镰孢菌醇、杂色曲霉素、黄曲霉毒素B1、黄曲霉毒素B2、黄曲霉毒素G1、黄曲霉毒素G2、曲酸、青霉酸、橘青霉素、黄曲霉毒素M1)多残留的超高效液相色谱-串联质谱(UPLCMS/MS)的检测方法。芝麻酱样品经乙腈-水-乙酸(80∶19∶1,体积分数,下同)溶液提取,QuEChERS方法净化后,以含0.1%甲酸的水溶液与乙腈为流动相,经ACQUITY UPLC BEH C18色谱柱(2.1mm×50mm,1.7μm)分离,以多级反应监测(MRM),外标法定量。结果表明:16种真菌毒素的检出限为0.03~1.5μg/kg,定量限为0.10~4.0μg/kg;线性范围在0.10~200μg/kg内各成分的线性相关系数均大于0.991;样品在定量限1倍、2倍和10倍3个添加水平下的平均回收率为70.7%~99.2%,相对标准偏差(RSD)为3.26%~10.5%。该方法简便、灵敏、快速,可用于芝麻酱中多种真菌毒素污染的监控分析。

关键词(KeyWords)： 芝麻酱;真菌毒素;液相色谱-串联质谱;分散固相萃取

Abstract:

Keywords:

基金项目(Foundation): 潍坊市科学技术发展计划(2014RKX094);; 山东省高等学校科技计划(J16LE52)

作者(Author): 郭礼强,崔晓娜,丁葵英,孙朝红,王亮亮,刘春雪

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