红树莓冻干粉中酚类化合物含量测定及其抗氧化活性研究Determination of Phenolic Compounds and Antioxidant Activity of Rubus idaeus L. Lyophilized Powder
王娜,韩海霞,钟志明,王娟
摘要(Abstract):
近年来,随着对具有抗氧化特性的水果生化含量的研究的深入,人们对水果在人类健康和营养中的重要作用有了更深层次的认识。虽然前人对多种水果蔬菜的酚类化合物含量和抗氧化能力进行了比较深入的研究,但目前对红树莓中的酚类化合物的含量和抗氧化能力的高低,以及对人类健康和营养的好处还未见报道。该研究首次采用紫外分光光度法(ultraviolet absorption spectrophotometry, UV)和高效液相色谱法(high performance liquid chromatography, HPLC)测定了红树莓冻干粉中酚类成分的种类及含量,并进行了方法学考察。结果显示该方法具有良好的线性范围、重复性、稳定性、精密度和加样回收率,并通过羟基自由基清除率、DPPH自由基清除率、ABTS~+自由基清除率和还原能力研究其抗氧化活性。采用UV法测得红树莓冻干粉中总多酚、总黄酮和花青素的含量分别为72.2,10.84,0.18 mg/g; HPLC法测得没食子酸、儿茶素、原花青素B_2、芦丁、鞣花酸和槲皮素的含量分别为0.41,0.62,0.81,0.37,0.64,0.014μg/g。通过抗氧化活性的测定,发现槲皮素、儿茶素、芦丁和没食子酸对ABTS~+·和羟基自由基的清除能力影响较大,而芦丁、没食子酸和原花青素B_2对还原力和DPPH·的影响较大,而鞣花酸对抗氧化活性均无较大的影响,说明红树莓冻干粉中芦丁和没食子酸的含量对抗氧化活性影响较为明显。表明红树莓冻干粉具有较好的抗氧化能力,因此红树莓冻干粉可作为功能性食品被进一步开发利用。
关键词(KeyWords): HPLC法;红树莓冻干粉;酚类物质;抗氧化
基金项目(Foundation):
作者(Author): 王娜,韩海霞,钟志明,王娟
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