海藻糖对高湿挤压肉类类似物品质特性的影响Effect of Trehalose on Quality Characteristics of Meat Analogs by High Humidity Extrusion
朱来景,刘萌,王彦丽,赵祥忠
摘要(Abstract):
为改善大豆蛋白肉类类似物的纤维化度和其他品质特性,探究高湿挤压过程中海藻糖(trehalose, TR)的添加比例对大豆蛋白肉类类似物品质的影响,采用湿法挤压技术制作大豆蛋白肉类类似物,分析TR的不同添加比例对大豆蛋白肉类类似物色泽、质构特性、流变特性和微观结构的影响。此外,还分析了TR的添加对肉类类似物中蛋白质二级结构的影响。结果表明,添加了6%TR的肉类类似物的硬度为(11.82±0.53) kgf,咀嚼度为(10.02±0.39) kgf,并且表现出了最高的纤维化度,为1.99±0.05,相比于未添加TR的样品增加了22%。表观观察到所有添加了TR的样品外观较0%TR的样品更平整光滑。此外,还发现添加了6%TR的样品的亮度值为56.68±0.19,最接近于熟制鸡胸肉的亮度值56.34±0.40。扫描电镜结果显示,添加了6%TR的肉类类似物相较于0%TR的样品有着更明显的各向异性结构。总而言之,肉类类似物中TR的最佳添加比例为6%,并且TR是制备高品质特性大豆蛋白肉类类似物的一种较理想的添加剂。
关键词(KeyWords): 肉类类似物;高水分挤压;海藻糖;大豆蛋白;品质特性
基金项目(Foundation): 山东省重点研发计划(2020CXGC010604)
作者(Author): 朱来景,刘萌,王彦丽,赵祥忠
参考文献(References):
- [1]MAZLAN M M,TALIB R A,CHIN N L,et al.Physical and microstructure properties of oyster mushroom-soy protein meat analog via single-screw extrusion[J].Foods,2020,9(8):1023.
- [2]马文平,李建新,朱辉,等.人造肉制备技术的研究进展[J].中国食品工业,2023(7):89-91.
- [3]TAN Y,ZHANG Z,MCCLE D J.Preparation of plant-based meat analogs using emulsion gels:lipid-filled RuBisCo protein hydrogels[J].Food Research International,2023,167:112708.
- [4]TAGHIAN D S,BROEKEMA N L,BOOM R,et al.Investigation potential of hydrocolloids in meat analogue preparation[J].Food Hydrocolloids,2023,135:108199.
- [5]胡小静,耿家圣,李绍平,等.魔芋仿生鸡肉的研制[J].食品工业科技,2006(8):101-102,105.
- [6]张雷达.海藻糖的应用及产业化发展前景[J].现代食品,2022,28(7):106-109.
- [7]胡云峰,王晓彬,魏增宇,等.海藻糖对米线品质改良技术研究[J].中国食品添加剂,2018(10):132-139.
- [8]康立宁,田志刚,刘香英,等.大豆品种品质特性与组织化蛋白产品品质特性的关系研究[J].粮食与饲料工业,2010(5):13-17.
- [9]YOUNUS B M,MIR I,SINGH L R,et al.Trehalose promotes structural disorderness of intrinsically disordered casein proteins with enhanced chaperone function[J].Journal of Molecular Liquids,2023,385:122270.
- [10]XIE G,TIMASHEFF S N.The thermodynamic mechanism of protein stabilization by trehalose[J].Biophysical Chemistry,1997,64(1-3):25-43.
- [11]DEKKERS B L,BOOM R M,VAN DER GOOT A J.Structuring processes for meat analogues[J].Trends in Food Science and Technology,2018,81:25-36.
- [12]LIN W J,LIU H Z,SHI A M,et al.Preparation and characterisation of films from xylose-glycosylated peanut protein isolate powder[J].International Journal of Food Science & Technology,2015,50(7):1538-1544.
- [13]CHEN Q,ZHANG J,ZHANG Y,et al.Rheological properties of pea protein isolate-amylose/amylopectin mixtures and the application in the high-moisture extruded meat substitutes[J].Food Hydrocolloids,2021,117:106732.
- [14]FANG Y,ZHANG B,WEI Y.Effects of the specific mechanical energy on the physicochemical properties of texturized soy protein during high-moisture extrusion cooking[J].Journal of Food Engineering,2014,121:32-38.
- [15]XIA S,XUE Y,XUE C,et al.Structural and rheological properties of meat analogues from Haematococcus pluvialis residue-pea protein by high moisture extrusion[J].LWT-Food Science and Technology,2022,154:112756.
- [16]ZHANG J,CHEN Q,LIU L,et al.High-moisture extrusion process of transglutaminase-modified peanut protein:effect of transglutaminase on the mechanics of the process forming a fibrous structure[J].Food Hydrocolloids,2021,112:106346.
- [17]CHANG Y Y,LI D,WANG L J,et al.Effect of gums on the rheological characteristics and microstructure of acid-induced SPI-gum mixed gels[J].Carbohydrate Polymers,2014,108:183-91.
- [18]ZHANG J,LIU L,JIANG Y,et al.High-moisture extrusion of peanut protein-/carrageenan/sodium alginate/wheat starch mixtures:effect of different exogenous polysaccharides on the process forming a fibrous structure[J].Food Hydrocolloids,2020,99:105311.
- [19]LI S,LUO Z,GUAN X,et al.Effect of ultrasonic treatment on the hydration and physicochemical properties of brewing rice[J].Journal of Cereal Science,2019,87:78-84.
- [20]YU L,RAMASWAMY H S,BOYE J.Protein rich extruded products prepared from soy protein isolate-corn flour blends[J].LWT-Food Science and Technology,2013,50(1):279-289.
- [21]ZAHAARI I,FERAWATI F,HELSTAD A,et al.Development of high-moisture meat analogues with hemp and soy protein using extrusion cooking[J].Foods,2020,9(6):772.
- [22]YASIR S B M,SUTTON K H,NEWBERRY M P,et al.The impact of Maillard cross-linking on soy proteins and tofu texture[J].Food Chemistry,2007,104(4):1502-1508.
- [23]康立宁,刘香英,田志刚,等.大豆蛋白挤压组织化过程中生物大分子之间的相互作用[J].大豆科技,2009(5):54-57.
- [24]BORTNOWSKA G,KRZEMINSKA N,MOJKA K.Effects of waxy maize and potato starches on the stability and physicochemical properties of model sauces prepared with fresh beef meat[J].International Journal of Food Science & Technology,2013,48(12):2668-2675.
- [25]BUENO A S,PEREIRA C M,MENEGASSI B,et al.Effect of extrusion on the emulsifying properties of soybean proteins and pectin mixtures modelled by response surface methodology[J].Journal of Food Engineering,2009,90(4):504-510.
- [26]WU W,LI F,WU X.Effects of rice bran rancidity on oxidation,structural characteristics and interfacial properties of rice bran globulin[J].Food Hydrocolloids,2021,110:106123.
- [27]HOSSAIN B F,CHAY S Y,MUHAMMAD K,et al.Structural and rheological changes of texturized mung bean protein induced by feed moisture during extrusion[J].Food Chemistry,2021,344:128643.
- [28]姚海霞,齐斌,黄友如,等.竹豆清蛋白的营养价值及基本理化性质[J].现代食品科技,2018,34(5):41-46.
- [29]李振,相海,赵有斌,等.植物蛋白螺杆挤压组织化技术的发展[J].中国油脂2023,48(9):67-74.
- [30]CHOI S M,MA C Y.Structural characterization of globulin from common buckwheat (Fagopyrum esculentum Moench) using circular dichroism and Raman spectroscopy[J].Food Chemistry,2007,102(1):150-160.
- [31]NIU M,XIONG L,ZHANG B,et al.Comparative study on protein polymerization in whole-wheat dough modified by transglutaminase and glucose oxidase[J].LWT-Food Science and Technology,2018,90:323-330.