19种加工辣椒辣椒素提取、纯化及其含量测定Extraction, Purification and Content Determination of Capsaicins from 19 Kinds of Processed Pepper
霍瑞春,王国泽,姚慧静,袁鹤,周刚,程立坤
摘要(Abstract):
目的:对19种加工辣椒进行辣椒素提取、纯化及含量测定。方法:辣椒素采用微波提取法提取,大孔树脂纯化,高效液相色谱法测定。色谱条件:Acclaim~(TM) C_(18)色谱柱(4.6 mm×250 mm, 5μm),流动相为水-乙腈(30∶70,V/V),流速:1.0 mL/min,检测波长:280 nm,柱温:30℃。结果:辣椒素最佳提取工艺为乙醇体积分数80%、提取时间20 min、微波功率300 W、料液比1∶12。辣椒素含量在10~100μg/mL,二氢辣椒素含量在2~50μg/mL,待测物与峰面积有良好的线性关系。辣椒素标准曲线的回归方程是Y=0.0837X+1.5245,R~2=0.9941,二氢辣椒素标准曲线的回归方程是Y=0.063X+0.0383,R~2=0.9991。结果表明,19个加工辣椒品种中艳椒425辣椒素类物质含量最高,为0.353 mg/g, TL 8-1-2辣椒素类物质含量最低,为0.021 mg/g。结论:比较得出不同辣椒品种之间辣椒素及辣度差异,找到适合不同辣度辣椒的加工生产方法,并为辣椒的良种选育及原料加工提供一定的参考依据。
关键词(KeyWords): 辣椒素;二氢辣椒素;提取;纯化;含量测定;加工方式
基金项目(Foundation): 内蒙古自治区科技计划项目(0904052001)
作者(Author): 霍瑞春,王国泽,姚慧静,袁鹤,周刚,程立坤
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