吴丹,杨苗苗,杜小平,祁蒙,杨水云

• 1:陕西学前师范学院生命科学与食品工程学院
• 2:安康市富硒产品研发中心
• 3:西安交通大学生命科学与技术学院

摘要(Abstract)：

纳豆激酶(nattokinase, NK)是纳豆芽孢杆菌发酵产生的一种丝氨酸蛋白酶，有良好的溶解血栓作用。但目前市面上NK产品纯度较低，需要食用大量粗品才能达到预防血栓的作用，因此，提高NK产品的纯度显得非常必要和重要。对发酵培养基组分进行优化，优化后的天然培养基组成为豆粕3%、甘油0.5%、谷氨酸钠1%、硫酸镁0.5%,在此条件下，发酵NK产量可达到约1 486.6 Fu/mL,满足扩大生产要求。在下游纯化技术研究方面，首先调高pH至10,然后采用十六烷基三甲基溴化铵(hexadecyl trimethyl ammonium bromide, CTAB)聚合沉淀NK的伴随产物γ-聚谷氨酸(γ-polyglutamic acid,γ-pGA),在此基础上，利用三项夹心法浓缩NK,透析冻干后获得效价高达(7.88±0.76)×10~5 Fu/g的高纯NK产品。该技术为生产高品质NK产品提供了重要的技术思路。

关键词(KeyWords)： 高纯纳豆激酶;碱性条件;沉淀伴随产物;培养基优化

Abstract:

Keywords:

基金项目(Foundation): 中国富硒产业研究院富硒专项研发计划项目(2021FXZX07-02);; 陕西省教育厅2021年度自然科学基金项目(21JK0586);; 陕西高校青年创新团队“秦岭药食同源生物资源保护与利用创新团队”资助;; 陕西学前师范学院食品科学与工程重点学科资助

作者(Author): 吴丹,杨苗苗,杜小平,祁蒙,杨水云

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