翟宗德,吴小梅

• 1:华东师范大学生命科学学院
• 2:上海应用技术学院化学与环境工程学院

摘要(Abstract)：

由于近期发生的一系列涉及食醋掺伪的事件,使得食醋的质量安全问题成为社会关注的焦点问题之一。开发食醋中掺入工业醋酸的检测方法是对食醋掺伪进行鉴定的关键手段。现有的国家标准采用放射性同位素检测方法,需要特殊的检测设备与条件,目前全国范围内只有复旦大学放射医学研究所具有检测资质,因此该技术在全国的普及尚有较大的局限性。稳定同位素分析技术,特别是点特异性天然同位素分馏核磁共振技术(SNIF-NMR)与同位素比率质谱仪测定技术(IRMS)在食醋质量鉴别方面的应用研究在国际上已经取得了一系列研究成果。两种技术结合能够对食醋的生产原料来自于C3或C4植物进行溯源,能够鉴定食醋中是否掺入了工业醋酸成分。文章对稳定同位素技术在食醋质量鉴别方面的最新研究进展进行了综述。

关键词(KeyWords)： 同位素质谱;点特异性天然同位素分馏核磁共振;食醋;工业醋酸;掺伪

Abstract:

Keywords:

基金项目(Foundation): 上海应用技术学院引进人才科研启动项目资助(YJ2011)

作者(Author): 翟宗德,吴小梅

参考文献(References)：

• [1]林崇德,姜璐,王德胜,等.中国成人教育百科全书-食醋[M].海口:南海出版公司,1994.
• [2]东耳.中国四大名醋[J].科学之友,2006(10):37.
• [3]Eric J,Karine W.Multielement,multicomponent,andmultisite isotopic profiling for food and beverage authentica-tion[J].Application Note,2003(1):24-25.
• [4]E C Regulation.Detecting enrichment of grape musts,con-centrated grape musts,rectified concentrated grape mustsand wines by application of nuclear magnetic resonance ofdeuterium(SNIF-NMR)[R].Official Journal of the Euro-pean Communities,1990,L272(33):64-73.
• [5]Remaud G,Debon A A,Martin Y L,et al.Authenticationof bitter almond oil and cinnamon oil:application of theSNIF-NMR method to benzaldehyde[J].Journal of Agricul-tural and Food Chemistry,1997,45(10):4042-4048.
• [6]Martin G G,Wood R,Martin G J.Detection of added beetsugar in concentrated and single strength fruit juices by deu-terium nuclear magnetic resonance(SNIF NMR Method):Collaborative study[J].The Journal of AOAC INTERNA-TIONAL,1996,79(4):917-928.
• [7]Martin G J,Martin M L,Mabon F,et al.A new methodfor the identification of the origin of ethanols in grain andfruit spirits:high-field quantitative deuterium nuclear mag-netic resonance at the natural abundance level[J].Journalof Agricultural and Food Chemistry,1981,31:311-315.
• [8]Martin G J,Guillou C,Martin M L,et al.Natural factors ofisotope fractionation and the characterization of wines[J].Journal of Agricultural and Food Chemistry,1988,36:316-322.
• [9]蒋露,薛洁,林奇,等.SNIF-NMR和IRMS技术在葡萄酒质量评价中的初步研究[J].食品发酵与工业,2008,34(11):139-143.
• [10]Monetti A,Reniero F,Versini G.Interregional and inter-annual variability of ethanol site-specific deuterium contentin Italian wines[J].Lebensm Unters Forsch,1994(199):311-316.
• [11]Gimenez-Miralles J E.Regional origin assignment of redwines from valencia(Spain)by 2 H NMR and 13 C IRMSstable isotope analysis of fermentative ethanol[J].Journalof Agricultural and Food Chemistry,2001(47):2645-2652.
• [12]Kosir I J,Kocjancic M,Ogrinc N,et al.Use of SNIF-NMR and IRMS in combination with chemometric methodsfor the determination of chaptalisation and geographical or-igin of wines(the example of Slovenian wines)[J].Ana-lytica Chimica Acta,2001(429):195-206.
• [13]Fauhl C,Wittkowski R.Oenological influences on the D/Hratios of wine ethanol[J].Journal of Agricultural and FoodChemistry,2000,48(9):3979-3984.
• [14]White J W,Doner L W.Mass spectrometric detection ofhigh-fructose corn syrup in honey by use of 13 C/12 C ratio:collaborative study[J].The Journal of AOAC INTERNA-TIONAL,1978,61:746-750.
• [15]White J W.High-fructose corn syrup adulteration of hon-ey:confirm atory testing required with certain isotope ratiovalues[J].The Journal of AOAC INTERNATIONAL,1980,63:1168-1170.
• [16]Rossmann A,Rieth W,Schmidt H L.Moglichkeiten undErgebnisse der Kombination von Messungen der Verhlt-nisse stabiler Wasserstoff-und Kohlenstoff-Isotope mit Re-sultaten konventioneller Analysen(RSK-Werte)zumNachweis des Zuckerzusatzes zu Fruchtsften[J].Zeitschrift für Lebensmitteluntersuchung und-ForschungA,1990,191:259-264.
• [17]Schmidt O,Quilter J M,Bahar B,et al.Inferring the ori-gin and dietary history of beef from C,N and S stable ra-tion analysis[J].Food Chemistry,2005,91:545-549.
• [18]Boner M,Forstel H.Stable isotope variation as a tool totrace the authenticity of beef[J].Analytical and Bioanalyt-ical Chemistry,2004,378:301-310.
• [19]王慧文,杨曙明,吴伟用.稳定同位素质谱技术检测肉鸡色素的来源[J].分析测试学报,2007,26(5):608-611.
• [20]Guillou C,Vallet C,Martin G J.A coupled NMR and MSisotopic method for the authentication of natural vinegars[J].Fresenius Journal Analytical Chemistry,1992,342:457-461.
• [21]Armin H.Determination of D/H isotope ratio in aceticacid from vinegars and pickled products by 2 H-NMR-spec-troscopy.European Food Research and Technology,2001,212:683-686.
• [22]Ryota H,Keita Y,Hiroki S,et al.Measurement of the I-sotope Ratio of Acetic Acid in Vinegar by HS-SPME-GC-TC/C-IRMS[J].Journal of Agricultural and Food Chem-istry,2010,58:7115-7118.
• [23]Freddy T,Eric J.2 H NMR and 13 C-IRMS analyses of ace-tic acid from vinegar,18 O-IRMS analysis of water in vine-gar:International collaborative study report[J].AnalyticaChimica Acta,2009,649:98-105.
• [24]Ryota H,Keita Y,Makiko K.Intramolecular Carbon Iso-tope Distribution of Acetic Acid in Vinegar[J].Journal ofAgricultural and Food Chemistry,2011,59:9049-9053.