不同方法制备酱渣可溶性膳食纤维及其结构、抗氧化活性研究Preparation of Soluble Dietary Fiber from Soy Sauce Residue by Different Methods and Study on Its Structure and Antioxidant Activity
许树荣,夏忠悦,邓莎,何贵萍,金雨欣,吕远平
摘要(Abstract):
以酱渣为原料,通过中性高温蒸煮、酸性高温蒸煮、纤维素酶酶解、中性蒸煮联合酶解4种方法制备酱渣可溶性膳食纤维(SDF),并对比了其结构和抗氧化活性。结果表明,4种方法对酱渣SDF的官能团组成没有影响。酸性高温蒸煮与纤维素酶酶解降低SDF的分子量效果最好。纤维素酶酶解能提高SDF的葡萄糖、甘露糖组成比例,蒸煮条件下SDF的半乳糖醛酸组成比例较高。纤维素酶酶解得到的SDF表面呈多孔结构,其余样品表面结构致密。体外抗氧化活性结果显示,中性高温蒸煮制备得到的SDF ABTS~+·清除能力与还原能力最高,ABTS~+·清除能力的IC_(50)值为0.77 mg/mL,远低于市售可溶性大豆多糖。
关键词(KeyWords): 酱渣;不同方法;SDF;结构;抗氧化
基金项目(Foundation):
作者(Author): 许树荣,夏忠悦,邓莎,何贵萍,金雨欣,吕远平
参考文献(References):
- [1]王帅.酱油渣中色素和大豆异黄酮的提取与性质研究[D].广州:华南农业大学,2020.
- [2]刘秋艳,连欣悦,容格清,等.膳食纤维生理功能研究进展[J].粮食与食品工业,2021,28(4):25-28.
- [3]ANDERSON J W,BAIRD P,DAVIS R H,et al.Health benefits of dietary fiber[J].Nutrition Reviews,2009,67(4):188-205.
- [4]徐燕,谭熙蕾,周才琼.膳食纤维的组成、改性及其功能特性研究[J].食品研究与开发,2021,42(23):211-218.
- [5]张岱玉.豆渣中果胶类多糖和膳食纤维的制备及其性能研究[D].广州:华南理工大学,2022.
- [6]曹心亭,毕海心,孙媛,等.加工方式对贝柱模拟消化产物中多糖结构和抗氧化活性的影响[J].中国食品添加剂,2023,34(5):88-95.
- [7]曾维才,石碧.天然产物抗氧化活性的常见评价方法[J].化工进展,2013,32(6):1205-1213.
- [8]HU X X,YU C X,SHOKOUH A,et al.Optimization of high-pressure processing-assisted extraction of pectic polysaccharides from three berries[J].Food Quality and Safety,2022,6(3):1-12.
- [9]沈琳.大黄茎可溶性膳食纤维的提取、改性及其应用[D].绵阳:西南科技大学,2022.
- [10]ZHANG W T,XU S J,GAO M,et al.Profiling the water soluble pectin in clear red raspberry (Rubus idaeus L.cv.Heritage) juice:impact of high hydrostatic pressure and high-temperature short-time processing on the pectin properties[J].Food Hydrocolloids,2022,125(1):107439.
- [11]SONG Y,LEI J,LI J,et al.Structural properties and biological activities of soluble dietary fibers rich in pectic-polysaccharides from different buckwheat green leaves[J].International Journal of Biological Macromolecules,2023,253(6):126686.
- [12]刘良伟,任杰,侯俊财,等.牡蛎多糖结构特征、理化特性及抗氧化活性研究[J].食品与发酵工业,2023,49(6):57-63.
- [13]FENG X Y,KASHIF A,JIANG G H,et al.Effects of extraction methods on the structural characteristics and functional properties of dietary fiber extracted from papaya peel and seed[J].Frontiers in Sustainable Food Systems,2024,8:1-13.
- [14]LI H Q,LONG D Q,PENG J L,et al.A novel in-situ enhanced blasting extrusion technique-extrudate analysis and optimization of processing conditions with okara[J].Innovative Food Science & Emerging Technologies,2012,16:80-88.
- [15]LIANG Z,LI K C,HUANG W W,et al.Production,structural and functional characteristics of soluble dietary fiber from fermented okara by Penicillium expansum[J].International Journal of Biological Macromolecules,2023,253(18):126621.
- [16]王飞运.大豆皮多糖提取及干法接枝改性研究[D].郑州:河南工业大学,2016.
- [17]郝倩,邓乾春,周彬,等.植物细胞壁多糖高效酶解技术及其在食品加工中应用研究进展[J].食品科学,2024,45(12):304-314.
- [18]LIU X X,GU L B,ZHANG G J,et al.Structural characterization and antioxidant activity of polysaccharides extracted from Chinese yam by a cellulase-assisted method[J].Process Biochemistry,2022,121:178-187.
- [19]王欣.亚临界水法提取苹果渣果胶多糖分离纯化及其结构解析研究[D].咸阳:西北农林科技大学,2018.
- [20]TANG J,YANG C R,QIN X T,et al.Effects of cellulose-degrading fungus Penicillium griseofulvum on the structure characteristics and adsorption properties of soluble dietary fiber from Citrus aurantium L[J].Food Bioscience,2023,55:102999.
- [21]刘子怡,李仪晴,张仁群,等.天然植物多糖抗氧化活性的研究进展[J].中国食物与营养,2024,30(8):40-46.
- [22]CHEN H,LIU Y,YANG T,et al.Interactive effects of molecular weight and degree of substitution on biological activities of arabinoxylan and its hydrolysates from triticale bran[J].International Journal of Biological Macromolecules,2021,166:1409-1418.
- [23]FUSO A,WINNIE D,LIEVE C,et al.DPPH radical scavenging activity of xylo-oligosaccharides mixtures of controlled composition:a step forward in understanding structure-activity relationship[J].Journal of Functional Foods,2023,101(1):105417.
- [24]HUANG S Q,DING S D,FAN L P.Antioxidant activities of five polysaccharides from Inonotus obliquus[J].International Journal of Biological Macromolecules,2012,50(5):1183-1187.
- [25]TAN X Q,CHENG X X,MA B Y,et al.Characterization and function analysis of soluble dietary fiber obtained from radish pomace by different extraction methods[J].Molecules,2024,29(2):500.
- [26]许涵婷,唐语谦,胡腾根,等.荔枝果渣可溶性膳食纤维去结合酚前后结构和功能性质的比较[J].现代食品科技,2023,39(8):206-212.