刘赵,沈海军,周凌晨,曹仲文

• 1:扬州大学旅游烹饪学院
• 2:扬州大学中餐非遗技艺传承文化和旅游部重点实验室
• 3:江苏省淮扬菜产业化工程中心
• 4:扬州旅游商贸学校

摘要(Abstract)：

可溶性大豆多糖是一种从豆制品加工副产物豆渣中提取的酸性多糖，具有良好的抗淀粉老化、抗淀粉黏结、乳化和乳化稳定性、蛋白质稳定性、成膜性等优异的食品加工特性，同时还具有降血糖、降血脂、抗氧化和预防癌症等生理功能，在食品、医药、保健等行业都有广泛的应用前景。文章不仅综述了可溶性大豆多糖传统的提取、分离纯化方法及其原理，而且介绍了两种新型大豆多糖提取工艺：电解水提取法和高压脉冲电场辅助提取法。最后对可溶性大豆多糖在食品中的应用及相应作用机制做了简述，以期为豆渣的高值利用、可溶性大豆多糖的提取及实际应用提供参考。

关键词(KeyWords)： 可溶性大豆多糖;多糖提取;电解水提取法;高压脉冲电场辅助提取;分离纯化

Abstract:

Keywords:

基金项目(Foundation): 中餐非遗技艺传承文化和旅游部重点实验室开放课题(WLB2202)

作者(Author): 刘赵,沈海军,周凌晨,曹仲文

参考文献(References)：

• [1]FURUTA H,MAEDA H.Rheological properties of water-soluble soybean polysaccharides extracted under weak acidic condition[J].Food Hydrocolloids,1999,13(3):267-274.
• [2]NAKAMURA A,FURUTA H,MAEDA H,et al.Analysis of structural components and molecular construction of soybean soluble polysaccharides by stepwise enzymatic degradation[J].Bioscience,Biotechnology,and Biochemistry,2001,65(10):2249-2258.
• [3]NAKAMURA A,FURUTA H,MAEDA H,et al.Analysis of the molecular construction of xylogalacturonan isolated from soluble soybean polysaccharides[J].Bioscience,Biotechnology,and Biochemistry,2002,66(5):1155-1158.
• [4]靳蔷薇.天然高分子乳化剂的比较研究[D].上海：上海交通大学，2017.
• [5]FUNAMI T,NAKAUMA M,NODA S,et al.Effects of some anionic polysaccharides on the gelatinization and retrogradation behaviors of wheat starch:soybean-soluble polysaccharide and gum arabic[J].Food Hydrocolloids,2008,22(8):1528-1540.
• [6]MATSUMURA Y,EGAMI M,SATAKE C,et a1.Inhibitory effects of peptide-bound polysaccharides on lipid oxidation in emulsions[J].Food Chemistry,2003(83):107-119.
• [7]宋慧，苗敬芝，董玉玮.双酶法提取大豆多糖及其抗氧化性研究[J].中国食品添加剂，2011(5):89-93.
• [8]NAKAMURA A,FURUTA H,KATO M,et al.Effect of soybean soluble polysaccharides on the stability of milk protein under acidic conditions[J].Food Hydrocolloids,2003,17(3):333-343.
• [9]钟碧疆.可溶性大豆多糖絮凝性及机理研究[D].广州：华南理工大学，2011.
• [10]刘塞纳.水溶性大豆多糖甲氧基含量对其成膜性能的影响[J].中国油脂，2018,43(4):130-134.
• [11]赵启竹.可溶性大豆多糖对淀粉老化的抑制及影响因素研究[D].无锡：江南大学，2021.
• [12]傅晶依，王思琪，丁修庆，等.大豆多糖的提取、分离纯化与功能的研究进展[J].食品研究与开发，2020,41(13):213-218.
• [13]徐玉环，吴月芳.2019大豆食品重点加工企业调研报告[J].大豆科技，2019(6):37-40.
• [14]张振山，叶素萍，李泉，等.豆渣的处理与加工利用[J].食品科学，2004(10):400-406.
• [15]施翠娥，蒋立科，李苗苗.大豆多糖提取分离工艺的优化研究[J].安徽农学通报(上半月刊),2009,15(3):148-150.
• [16]桂雨豪，郭蔼明，杨锦杰，等.从豆渣中提取水溶性大豆多糖的工艺优化研究[J].中国酿造，2015,34(9):71-74.
• [17]朱丹，朱立斌，牛广财，等.沙果渣膳食纤维超高压处理条件优化及其微观结构表征[J].天然产物研究与开发，2020,32(1):118-126.
• [18]FAYAZ G,PLAZZOTTA S,CALLIGARIS S,et al.Impact of high pressure homogenization on physical properties,extraction yield and biopolymer structure of soybean okara[J].LWT-Food Science and Technology,2019,113:108324.
• [19]CHEN P C,LIN C,CHEN M H,et al.The micronization process for improving the dietary value of okara (soybean residue) by planetary ball milling[J].LWT-Food Science and Technology,2020,132:109848.
• [20]王曼宇，刘乃新，张福顺.植物源性多糖提取及生物活性研究进展[J].中国农学通报，2021,37(29):34-41.
• [21]ZHANG B,LI Y,ZHANG F,et al.Extraction,structure and bioactivities of the polysaccharides from Pleurotus eryngii:a review[J].International Journal of Biological Macromolecules,2020,150:1342-1347.
• [22]YAMAGUCHI F,KOJIMA H,MURAMOTO M,et al.Effects of hexametaphosphate on soybean pectic polysaccharide extraction[J].Bioscience,Biotechnology,and Biochemistry,1996,60(12):2028-2031.
• [23]熊杰，杨玥熹，华欲飞.豆渣水溶性大豆多糖提取工艺研究[J].大豆科学，2009,28(6):1119-1122.
• [24]韦巍，李雪华.多糖的研究进展[J].国外医学药学分册，2005,32(3):179-180.
• [25]WU G H,HU T,LI Z Y,et al.In vitro antioxidant activities of the polysaccharides from Pleurotus tuber-regium (Fr.) Sing[J].Food Chemistry,2014,148:351-356.
• [26]朱强，蒋建茹，林欣大.酸碱法提取黄粉虫中壳聚糖的要点[J].科技创新导报，2014,11(14):238.
• [27]孟岳成，邱蓉，张学兵.碱法提取可溶性大豆多糖的工艺[J].食品研究与开发，2009,30(11):83-86.
• [28]李利红，李凯，李向辉，等.刺五加多糖的提取工艺研究[J].饲料研究，2019,42(6):93-96.
• [29]俞明君，李苗苗，王金浩，等.杏鲍菇水溶性和碱溶性多糖提取工艺研究[J].食用菌，2017,39(6):78-80.
• [30]YE J,HUA X,ZHAO Q,et al.Characteristics of alkali-extracted peanut polysaccharide-protein complexes and their ability as Pickering emulsifiers[J].International Journal of Biological Macromolecules,2020,162:1178-1186.
• [31]王萱萱，刘春宇，谢贝昱，等.碱提甘蔗皮多糖提取工艺、初步结构及其对α-葡萄糖苷酶的抑制作用[J].中国农业科学，2021,54(12):2653-2665.
• [32]范文奇，要志宏，申丽媛，等.豆粕多糖的酶法提取工艺研究[J].中国调味品，2017,42(5):65-68,83.
• [33]CHENG M,QI J R,FENG J L,et al.Pea soluble polysaccharides obtained from two enzyme-assisted extraction methods and their application as acidified milk drinks stabilizers[J].Food Research International,2018,109:544-551.
• [34]SORIA A C,VILLAMIEL M.Effect of ultrasound on the technological properties and bioactivity of food:a review[J].Trends in Food Science & Technology,2010,21(7):323-331.
• [35]BARBERO G F,LIAZID A,PALMA M,et al.Ultrasound-assisted extraction of capsaicinoids from peppers[J].Talanta,2008,75(5):1332-1337.
• [36]郭孝武.超声提取分离[M].北京：化学工业出版社，2008.
• [37]RASO J,MANAS P,PAGAN R,et al.Influence of different factors on the output power transferred into medium by ultrasound[J].Ultrasonics Sonochemistry,1999,5(4):157-162.
• [38]FERANADEZ-LEON M F,FRENANDEZ-LEON A M,LOZANO M,et al.Altered commercial controlled atmosphere storage conditions for 'Parhenon' broccoli plants (Brassica oleracea L.var.italica).Influence on the outer quality parameters and on the health-promoting compounds[J].LWT-Food Science and Technology,2013,50(2):665-672.
• [39]KIKUCHI K,IOKA A,OKU T,et al.Concentration determination of oxygen nanobubbles in electrolyzed water[J].Journal of Colloid and Interface Science,2009,329(2):306-309.
• [40]侯睿.水溶性大豆多糖与超声波辅助冷冻协同改善草鱼鱼糜品质的研究[D].广州：华南理工大学，2018.
• [41]何玉红.豆渣中水溶性大豆多糖的提取及其在面包中的应用[D].合肥：合肥工业大学，2007.
• [42]VALACHOVIC P,PECHOVA A,MASON T J.Towards the industrial production of medicinal tincture by ultrasound assisted extraction[J].Ultrasonics Sonochemistry,2001,8(2):111-117.
• [43]郝继伟.超声波提取豆渣中水溶性大豆多糖工艺研究[J].大豆科学，2011,30(3):507-510.
• [44]田海娟，于晓平，季蕾蕾.Design-Expert设计超声波法提取豆渣中大豆多糖工艺优化[J].食品研究与开发，2014,35(24):66-70.
• [45]田瑞红，江连洲，胡少新，等.超声波酶法提取豆渣中水溶性多糖条件的优化[J].大豆科技，2019(1):179-183.
• [46]任花.微波辅助法提取豆渣中大豆多糖的工艺研究[J].轻工科技，2015,31(12):21-22,25.
• [47]王胜男，赵玲玲，张红运，等.提取方法对大豆种皮多糖乳化能力的影响[J].中国食品学报，2018,18(1):154-161.
• [48]范宏亮，赵玲玲，李君，等.微波辅助提取工艺对大豆种皮水溶性多糖-蛋白乳状液乳化活性及表面电位的影响[J].食品科学，2018,39(20):49-56.
• [49]谭兰芳，许文珍，杨跃歌，等.超临界CO2萃取白花蛇舌草粗多糖工艺研究[J].化工管理，2021(6):81-82.
• [50]杨孝辉，郭君.响应面法优化超临界CO2流体萃取淮山多糖工艺[J].食品工业科技，2019,40(7):185-189,198.
• [51]张继贤.亚临界水萃取温度对香菇多糖化学结构、链构象转变及生物活性的影响研究[D].镇江：江苏大学，2020.
• [52]娄冠群，张永忠，李振艳，等.亚临界水提取豆渣中可溶性大豆多糖工艺研究[J].中国油脂，2010,35(5):61-63.
• [53]赵彬，张海晖，张继贤，等.小麦胚芽多糖的亚临界水萃取的工艺研究[J].食品工业，2017,38(7):45-49.
• [54]谢慧琳，唐金艳，林育钊，等.电解水技术在采后果蔬保鲜中的应用研究进展[J].盐城工学院学报(自然科学版),2020,33(1):61-66.
• [55]赵月涵，杨盈悦，邓尚贵，等.冷杀菌技术在水产品保鲜中的应用研究进展[J].中国渔业质量与标准，2021,11(5):56-64.
• [56]翟娅菲，田佳丽，相启森，等.非热加工技术在果蔬保鲜中的应用[J].食品工业，2021,42(5):327-332.
• [57]韩晓梅，吴小禾，李文亚，等.电解水在利用虾壳提取甲壳素中的应用研究[J].食品研究与开发，2019,40(16):51-57.
• [58]李楚楚，赵丹丹，韩雪，等.利用酸性电解水提取柿子渣中果胶及其工艺优化[J].食品科技，2019,44(1):274-280.
• [59]袁森，杜明星，李博生.电解水替代强碱提取大豆渣可溶性多糖工艺优化研究[J].河北农业大学学报，2021,44(1):85-90.
• [60]汤国强，沙俊杰，黄宝军.油田污水处理中高压脉冲电场杀菌方法的研究[J].化工自动化及仪表，2015,42(8):901-903.
• [61]BAZHAL M I,NGADI M O,RAGHAVAN G S V,et al.Textural changes in apple tissue during pulsed electric field treatment[J].Journal of Food Science,2003,68(1):249-253.
• [62]LOHANI U C,MUTHUKUMARAPPAN K.Application of the pulsed electric field to release bound phenolics in sorghum flour and apple pomace[J].Innovative Food Science & Emerging Technologies,2016,35:29-35.
• [63]骆航，王文渊，尹素娟，等.响应面法优化高压脉冲电场提取火棘果活性成分[J].食品安全质量检测学报，2022,13(2):373-380.
• [64]华南理工大学.一种利用高压脉冲电场协同超声提取豆渣中水溶性大豆多糖的方法：中国，CN202110528203.8[P].2021-09-07.
• [65]侯越，包鸿慧.榛蘑多糖提取液脱蛋白方法的研究[J].农产品加工，2018(15):69-70,76.
• [66]桂雨豪.水压热法制备水溶性大豆多糖与其应用研究[D].广州：华南理工大学，2016.
• [67]向瑞琪，谢锋，李占彬.食用菌多糖提取、检测、生物活性与机制研究进展[J].黑龙江农业科学，2021(7):109-115.
• [68]苏浩，余以刚，杨海燕，等.膜分离技术在水溶性大豆多糖提取中的应用[J].食品工业科技，2009,30(8):216-217,220.
• [69]GU W,WANG Y,ZENG L,et al.Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet[J].Biomedicine & Pharmacotherapy,2020,125:109910.
• [70]MADAR Z.Effect of brown rice and soybean dietary fiber on the control of glucose and lipid metabolism in diabetic rats[J].The American Journal of Clinical Nutrition,1983,38(3):388-393.
• [71]ARUN K B,THOMAS S,RESHMITHA T R,et al.Dietary fibre and phenolic-rich extracts from Musa paradisiaca inflorescence ameliorates type 2 diabetes and associated cardiovascular risks[J].Journal of Functional Foods,2017,31:198-207.
• [72]LUO X,WANG Q,ZHENG B,et al.Hydration properties and binding capacities of dietary fibers from bamboo shoot shell and its hypolipidemic effects in mice[J].Food and Chemical Toxicology,2017,109:1003-1009.
• [73]HAN S F,JIAO J,ZHANG W,et al.Lipolysis and thermogenesis in adipose tissues as new potential mechanisms for metabolic benefits of dietary fiber[J].Nutrition,2017,33:118-124.
• [74]CHEW K Y,BROWNLEE I A.The impact of supplementation with dietary fibers on weight loss:a systematic review of randomised controlled trials[J].Bioactive Carbohydrates and Dietary Fibre,2018,14:9-19.
• [75]ZHOU R,REN Z,YE J,et al.Fermented soybean dregs by neurospora crassa:a traditional prebiotic food[J].Applied Biochemistry and Biotechnology,2019,189(2):608-625.
• [76]白冰瑶，刘新愚，周茜，等.红枣膳食纤维改善小鼠功能性便秘及调节肠道菌群功能[J].食品科学，2016,37(23):254-259.
• [77]MAETA A,KSTSKAWA M,INOMOTO Y,et al.Intake of okara soup for 2 weeks for breakfast improved defecation habits in young Japanese women with self-reported constipation:a randomized,double-blind,placebo-controlled,intervention study[J].Journal of Food Science,2020,85(10):3570-3576.
• [78]HE Y,WANG B,WEN L,et al.Effects of dietary fiber on human health[J].Food Science and Human Wellness,2022,11(1):1-10.
• [79]TANG L,XU F,ZHANG T,et al.Dietary fibre intake associated with reduced risk of oesophageal cancer in Xinjiang,China[J].Cancer Epidemiology,2013,37(6):893-896.
• [80]王思远.水溶性大豆多糖的改性及在米粉中的应用研究[D].广州：华南理工大学，2011.
• [81]MAEDAA H,NAKAMURA A.Soluble Soybean Polysaccharide[M].Cambrrdge:Woodhead Publishing,2009:693-709.
• [82]LIU D,LI Z,FAN Z,et al.Effect of soybean soluble polysaccharide on the pasting,gels,and rheological properties of kudzu and lotus starches[J].Food Hydrocolloids,2019,89:443-452.
• [83]王雨，刘翀，洪静，等.可溶性大豆多糖对中筋小麦粉面团特性和生鲜面品质的影响[J].食品科技，2020,45(3):177-183.
• [84]刘传菊，赵玉，汤尚文，等.可溶性大豆多糖对碎米粉物化特性的影响[J].中国粮油学报，2021,36(9):7-13,21.
• [85]刘倩，吕远，邢胜杰，等.可溶性大豆多糖对面包品质的影响[J].食品科技，2013,38(5):179-182.
• [86]胡喜萍.可溶性大豆多糖对面粉和面团流变学及油条品质的影响[J].现代面粉工业，2019,33(6):52.
• [87]潘燕，钟昔阳，罗水忠，等.可溶性大豆多糖对春卷皮冻融循环过程中品质变化的影响[J].食品科学，2022,43(8):59-65.
• [88]BANDE R M,PRASAD B,MISHRA I M,et al.Oil field effluent water treatment for safe disposal by electroflotation[J].Chemical Engineering Journal,2008,137(3):503-509.
• [89]ISRAELACHVIL J N.Intermolecular and Surface Forces:Special Interactions:Hydrogen-Bonding and Hydrophobic and Hydrophilic Interactions[M].New York:Academic Press,2011:151-167.
• [90]PELAN B M C,WATTS K M,CAMPBELL I J,et al.The stability of aerated milk protein emulsions in the presence of small molecule surfactants[J].Journal of Dairy Science,1997,80(10):2631-2638.
• [91]LOBO L,SVEREIKA A.Coalescence during emulsification:2.Role of small molecule surfactants[J].Journal of Colloid and Interface Science,2003,261(2):498-507.
• [92]DICKINSON E.Hydrocolloids at interfaces and the influence on the properties of dispersed systems[J].Food Hydrocolloids,2003,17(1):25-39.
• [93]杨晓泉，齐军茹，司华静，等.大豆多糖的研究进展及在含乳饮料、米面等食品上的应用[J].中国食品添加剂，2008(3):135-139.
• [94]KIKUCHI T,YOKOTSUKA T.Studies on the polysaccharides from soy sauce:Part I.Purification and properties of two acidic polysaccharides[J].Agricultural and Biological Chemistry,1972,36(4):544-550.
• [95]徐洁茹，童群义.乙酰化可溶性大豆多糖的制备及其乳化性能研究[J].食品与发酵工业，2021,47(21):148-157.
• [96]TAVASOLI S,MAGHSOUDLOU Y,JAFARI S M,et al.Improving the emulsifying properties of sodium caseinate through conjugation with soybean soluble polysaccharides[J].Food Chemistry,2022(377):131987.
• [97]LOGAN A,DAY L,PIN A,et al.Interactive effects of milk fat globule and casein micelle size on the renneting properties of milk[J].Food and Bioprocess Technology,2014,7(11):3175-3185.
• [98]TREJO R,CORZO-MARTINEZ M,WILKINSON S,et al.Effect of a low temperature step during fermentation on the physico-chemical properties of fat-free yogurt[J].International Dairy Journal,2014,36(1):14-20.
• [99]朱丽娜，张立永，张敏，等.可溶性大豆多糖在酸性乳饮料中的应用[J].中国乳品工业，2009,37(4):33-35.
• [100]周森，何志勇，曾茂茂，等.可溶性大豆多糖对于配制型酸性乳饮料稳定效应影响因素研究[J].食品科技，2019,44(7):308-313.
• [101]汪洪涛，陈成，石雪萍，等.豆渣中水溶性大豆多糖的性质及应用研究[J].河南农业科学，2014,43(6):157-160.
• [102]廖文艳，徐致远，刘振民.大豆多糖在常温褐色乳酸菌饮品中的应用[J].江苏农业科学，2016,44(12):303-305.
• [103]黄宸，谢晶，薛斌，等.大豆多糖复合膜及其保鲜应用研究进展[J].食品与发酵工业，2021,47(10):251-258.
• [104]申亚倩，陈志周，安永超，等.纳米 TiO2 改性天然聚合物包装膜的研究进展[J].包装与食品机械，2014,32(6):60-63.
• [105]GHAZIHOSEINI S,ALIPOORMAZANDARANI N,NAFCHI A M.The effects of nano-SiO2 on mechanical,barrier,and moisture sorption isotherm models of novel soluble soybean polysaccharide films[J].International Journal of Food Engineering,2015,11(6):833-840.
• [106]SHAILI T,ABDORREZA M N,FARIBORZ N.Functional,thermal,and antimicrobial properties of soluble soybean polysaccharide biocomposites reinforced by nano TiO2[J].Carbohydrate Polymers,2015,134:726-731.
• [107]SALARBASHI D,TAFAGHODI M,BAZZAZ B S F,et al.Characterization of a green nanocomposite prepared from soluble soy bean polysaccharide/Cloisite 30B and evaluation of its toxicity[J].International Journal of Biological Macromolecules,2018,120:109-118.
• [108]SALARBASHI D,MORTAZAVIS A,NOGHABI M S,et al.Development of new active packaging film made from a soluble soybean polysaccharide incorporating ZnO nanoparticles[J].Carbohydrate Polymers,2016,140:220-227.
• [109]GHANI S,BARZEGAR H,NOSHAD M,et al.The preparation,characterization and in vitro application evaluation of soluble soybean polysaccharide films incorporated with cinnamon essential oil nanoemulsions[J].International Journal of Biological Macromolecules,2018,112:197-202.
• [110]LIU Q R,WANG W B,QI J R,et al.Oregano essential oil loaded soybean polysaccharide films:effect of Pickering type immobilization on physical and antimicrobial properties[J].Food Hydrocolloids,2019,87:165-172.
• [111]SALARBASHI D,TAJIK S,SHOJAEE-ALIABADI S,et al.Development of new active packaging film made from a soluble soybean polysaccharide incorporated Zataria multiflora Boiss and Mentha pulegium essential oils[J].Food Chemistry,2014,146:614-622.
• [112]曾维丽，孙于庆，石晓.大豆多糖复合膜保鲜草莓的研究[J].保鲜与加工，2018,18(6):51-56.
• [113]曾维丽，张锋杰.大豆多糖复合膜对红玫瑰葡萄的保鲜效果研究[J].中国果菜，2019,39(10):21-24,31.
• [114]高文宏，黄扬萍，曾新安.水溶性大豆多糖对鳙鱼鱼糜蛋白冷冻变性及结构的影响[J].华南理工大学学报(自然科学版),2018,46(11):16-22.