赵雯,彭黔荣,冯贵涛,杨敏,冯淑艳

• 1:贵州大学化学与化工学院
• 2:贵州中烟工业有限责任公司技术中心
• 3:贵州大学药学院

摘要(Abstract)：

分子印迹固相萃取技术是一种高效的样品前处理技术,能从复杂的样品中选择性分离目标物及其结构类似物,现已广泛应用于食品、医药、化工等多个领域。介绍了分子印迹聚合物的合成原理、方法及表征,分子印迹固相萃取的操作模式,并对近几年国内外分子印迹固相萃取技术在食品农药、兽药残留检测中的应用进行了总结。阐述了该技术目前存在的问题,并对其发展趋势进行展望,为其更好地应用于食品的分析检测提供参考。

关键词(KeyWords)： 分子印迹固相萃取;农药残留;兽药残留;食品安全;应用进展

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(21562014)

作者(Author): 赵雯,彭黔荣,冯贵涛,杨敏,冯淑艳

参考文献(References)：

• [1]王会枝,杨柳,沈妍铮,等.印迹固相萃取-HPLC对酱油中防腐剂的选择分析[J].河南工业大学学报(自然科学版),2011,32(2):40-44.
• [2]李春丽,严守雷.分子印迹固相萃取-高效液相色谱法测定酱油中酪胺含量[J].食品安全质量检测学报,2014(9):2728-2734.
• [3]杨红梅,刘艳琴,王浩,等.固相萃取-高效液相色谱法测定酱油中10种防腐剂[J].中国调味品,2011,36(5):94-96.
• [4]王新运,万新军,程乐华,等.高效液相色谱法同时测定酱油中的苯甲酸和山梨酸[J].应用化工,2010,39(9):1413-1416.
• [5]Wang S,Xu Z X,Fang G Z,et al.Synthesis and characterization of a molecularly imprinted silica-gel sorbent for the on-line determination of trace sudan I in chilli powder through high-performance liquid chromatography[J].Journal of Agricultural and Food Chemistry,2007,55(10):3869-3876.
• [6]苏日辉,阮贵华,曾令镇,等.超声辅助分散乳液微萃取/高效液相色谱法同时测定辣椒粉中的5种染色剂[J].分析测试学报,2015,34(5):546-551.
• [7]Haupt K,Mosbach K.Molecularly imprinted polymers and their use in biomimetic sensors[J].Chemical Reviews,2000,100(7):2495-2504.
• [8]Wulff G,Sarhan A,Zabrocki K.Enzyme-analogue built polymers and their use for the resolution of raeemates[J].Tetrahedron Letters,1973,14(44):4329-4332.
• [9]Li Ji,Hu Xiaoling,Guan Ping.Synthesis and application of molecularly imprinted nanoparticles combined ultrasonic assisted for highly selective solid phase extraction trace amount of celecoxib from human plasma samples using design expert(DXB)software[J].Ultrasonics Sonochemistry,2016,33:67-76.
• [10]Whitcombe M J,Rodriguez M E,Villar P,et al.A new method for the introduction of recognition site functionality into polymers prepared by molecular imprinting:synthesis and characterization of polymeric receptors for cholesterol[J].Journal of the American Chemical Society,1995,117(27):7105-7111.
• [11]Ndunda E N,Mizaikoff B.Synthesis of stationary phases that provide group recognition for polychlorinated biphenyls by porogenic fragment template imprinting[J].Journal of Separation Science,2016,39(5):939-946.
• [12]Sun Guangying,Wang Chao,Luo Yuqin,et al.Cost-effective imprinting combining macromolecular crowding and a dummy template for the fast purification of punicalagin from pomegranate husk extract[J].Journal of Separation Science,2016,39(10):1963-1970.
• [13]Arabi M,Ghaedi M,Ostovan A.Development of dummy molecularly imprinted based on functionalized silica nano-particles for determination of acrylamide in processed food by matrix solid phase dispersion[J].Food Chemistry,2016,210:78-84.
• [14]Xu Zhifeng,Deng Peihong,Li Junhua,et al.Construction of imprint sites in mesopores of SBA-15via thiol-ene click reaction[J].Food Chemistry,2016,210:78-84.
• [15]Luliński P,Klejn D,Maciejewska D.Stoichiometric molecularly imprinted polymers for the recognition of anti-cancer pro-drug tegafur[J].Materials Science and Engineering C,2016,65:400-407.
• [16]马明明,刘承龙,苏新科,等.基于分子印迹聚合物零流电位法识别邻甲苯胺[J].厦门大学学报(自然科学版),2016,55(1):9-15.
• [17]戴芳芳,周瑶,马良,等.间苯氧基苯甲酸分子印迹膜的电化学聚合制备及其应用[J].食品科学,2015,36(20):232-238.
• [18]Ersoya S K,Tutemb E,Baskan K S,et al.Preparation,characterization and usage of molecularly imprinted polymer for the isolation of quercetin from hydrolyzed nettle extract[J].Journal of Chromatography B,2016,210:78-84.
• [19]P Luliński,D Klejn,D Maciejewska.Synthesis and characterization of imprinted sorbent for separation of gramine from bovine serum albumin[J].Journal of Chromatography B,2016,65:400.
• [20]苏子豪.分子印迹固相萃取芯片的构建及其分析应用的研究[D].广州:广东药学院,2015.
• [21]Li Ji,Hu Xiaoling,Guan Ping.Preparation of L-phenylalanine imprinted solid-phase extraction sorbent by pickering emulsion polymerization and the selective enrichment of L-phenylalanine from human urine[J].Journal of Separation Science,2016,39(10):1863-1872.
• [22]周菊英,张玲玉,李鹏飞,等.以马来松香丙烯酸乙二醇酯为交联剂的分子印迹聚合物对槲皮素的选择吸附性能[J].精细化工,2016,33(3):314-319.
• [23]Li Hong,He Hongliang,Huang Jiaojiao,et al.A novel molecularly imprinted method with computational simulation for the affinity isolation and knockout of baicalein from Scutellaria baicalensis[J].Biomedical Chromatography,2015,30(2):117-125.
• [24]Song Renyuan,Hu Xiaoling,Guan Ping,et al.Surface modification of imprinted polymer microspheres with ultrathin hydrophilic shells to improve selective recognition of glutathione in aqueous media[J].Materials Science and Engineering C,2016,60:1-6.
• [25]吴宗远,李小燕,苏晓濛,等.分子印迹固相萃取在线检测技术在食品安全分析中的研究进展[J].食品安全质量检测学报,2014(5):1297-1304.
• [26]Lian Ziru,Wang Jiangtao.Determination of crystal violet in seawater and seafood samples through off-line molecularly imprinted SPE followed by HPLC with diode-array detection[J].Journal of Separation Science,2013,36(5):980-985.
• [27]Lian Ziru,Wang Jiangtao.Study of molecularly imprinted solid-phase extraction of gonyautoxins 2,3in the cultured dinoflagellate Alexandrium tamarense by high-performance liquid chromatography with fluorescence detection[J].Environmental Pollution,2013,182(6):385-391.
• [28]Lian Ziru,Li Haibei,Wang Jiangtao.Experimental and computational studies on molecularly imprinted solid-phase extraction for gonyautoxins 2,3from dinoflagellate Alexandrium minutum[J].Analytical and Bioanalytical Chemistry,2016,408(20):5527-5535.
• [29]Liu Meijiao,Li Yongna,Lin Shen,et al.Determination of tetracycline residues in lake water by on-line coupling of molecularly imprinted solid-phase extraction with high performance liquid chromatography[J].Analytical Methods,2014,6(23):9446-9452.
• [30]Qiu Xiuzhen,Liang Yong,Guo Huishi,et al.Determination of phenolic compounds in environmental water by HPLC combination with on-line solid-phase extraction using molecularly imprinted polymers[J].Journal of Nanoscience and Nanotechnology,2015,15(12):9578-9584.
• [31]Khan S,Bhatia T,Trivedi P,et al.Selective solid-phase extraction using molecularly imprinted polymer as a sorbent for the analysis of fenarimol in food samples[J].Food Chemistry,2016,199:870-875.
• [32]Li Xinpei,Wang Yuanpeng,Sun Qun,et al.Molecularly imprinted dispersive solid-phase extraction for the determination of triazine herbicides in grape seeds by high-performance liquid chromatography[J].Chromatographic Science,2016,54(5):871-877.
• [33]Andrade F N,Nazario C E D,Santos-neto A J,et al.Development of on-line molecularly imprinted solid phase extraction-liquid chromatography mass spectrometry for triazine analysis in corn samples[J].Analytical Methods,2016,8(5):1181-1186.
• [34]Wu Suqin,Xu Zhiguang,Yuan Qionghui,et al.Recognition characteristics of molecularly imprinted microspheres for triazine herbicides using hydrogen-bond array strategy and their analytical applications for corn and soil samples[J].Journal of Chromatography A,2011,1218(10):1340-1346.
• [35]汤凯洁,罗秋水,余瑞龙,等.分子印迹固相萃取/高效液相色谱法测定猪肝中盐酸克伦特罗的方法研究[J].分析测试学报,2016,35(1):115-118.
• [36]Song Xuqin,Zhou Tong,Liu Qingying,et al.Molecularly imprinted solid-phase extraction for the determination of ten macrolide drugs residues in animal muscles by liquid chromatography-tandem mass spectrometry[J].Food Chemistry,2016,208:169-176.
• [37]王凤美,陈军辉,林黎明,等.UPLC-MS/MS法对动物源性食品中12种大环内酯类抗生素残留的测定[J].分析测试学报,2009,28(7):784-788.
• [38]Lima M D,Vieira A C,Martins I,et al.On-line restricted access molecularly imprinted solid phase extraction of ivermectin in meat samples followed by HPLC-UV analysis[J].Food Chemistry,2016,197:7-13.
• [39]林冬,郭晶晶.氯霉素分子印迹整体柱的制备和表征[J].环境监测管理与技术,2016,28(1):50-53.
• [40]Samanidou V,Kehagia M,Kabir A,et al.Matrix molecularly imprinted mesoporous sol-gel sorbent for efficient solid-phase extraction of chloramphenicol from milk[J].Analytica Chimica Acta,2016,914:62-74.
• [41]蒋定国,杨大进,方从容,等.高效液相色谱法测定牛奶中氯霉素残留量的研究[J].中国食品卫生杂志,2003,15(1):35-36.
• [42]Tang Yimei,Li Min,Gao Xue,et al.Preconcentration of the antibiotic enrofloxacin using a hollow molecularly imprinted polymer,and its quantitation by HPLC[J].Microchim Acta,2016,183(2):589-596.
• [43]He Xiuping,Mei Xiaoqi,Wang Jiangtao,et al.Determination of diethylstilbestrol in seawater by molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography[J].Marine Pollution Bulletin,2016,102:142-147.
• [44]He Xiuping,Tan Liju,Wu Wei.Determination of sulfadiazine in eggs using molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography[J].Journal of Separation Science,2016,39(11):2204-2212.