王晓杰,刘晓兰,丛万锁,郑喜群,林巍

• 1:齐齐哈尔大学食品与生物工程学院黑龙江省普通高校农产品加工重点实验室

摘要(Abstract)：

以转谷氨酰胺酶为催化剂、D-氨基半乳糖为酰基受体,通过糖基化反应修饰玉米醇溶蛋白,以修饰产物中D-氨基半乳糖的导入量为指标,采用单因素实验优化糖基化反应条件,并对修饰产物的部分功能性质进行了研究。结果表明:D-氨基半乳糖糖基化修饰玉米醇溶蛋白的最优条件为初始pH 7.5,反应温度37℃,底物浓度3%,酰基供体与酰基受体的摩尔比1∶3,加酶量60U/g蛋白,反应时间10h。在此条件下,D-氨基半乳糖的接入量最大,为12.55mg/g蛋白。与玉米醇溶蛋白和交联玉米醇溶蛋白相比,糖基化修饰玉米醇溶蛋白的表面疏水性显著下降,表明其溶解性明显改善。

关键词(KeyWords)： 玉米醇溶蛋白;D-氨基半乳糖;转谷氨酰胺酶;糖基化;表面疏水性

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省自然科学基金项目(C201332);; 黑龙江省教育厅基本业务专项项目(135109259)

作者(Author): 王晓杰,刘晓兰,丛万锁,郑喜群,林巍

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