李娟娟,陈红,郭力强,李春秋,刘懋,冯镇

• 1:东北农业大学乳品科学教育部重点实验室
• 2:黑龙江省克东腐乳有限公司

摘要(Abstract)：

从我国传统细菌发酵腐乳中筛选出一株对大豆蛋白具有良好水解活性的玫瑰考克氏菌(Kocuria rosea)KDF3。在研究了该菌株胞外蛋白酶KRP3的酶学性质基础上,利用氨基酸检测技术分析了该酶水解大豆蛋白产生的氨基酸指纹图谱。实验结果表明,该酶的最适反应温度和pH分别为50℃和8.0,在20~40℃和pH 5.0~7.0具有良好的稳定性,60℃保温30min即可灭活;对10%的乙醇和11%的NaCl均有很好的耐受性;Ca2+和Mn2+对该酶激活作用显著。由该酶水解大豆蛋白产生的氨基酸图谱可知,总游离氨基酸、必需氨基酸和风味氨基酸的含量均明显增加。由以上结果可知,该酶在酶法促熟和酶法发酵腐乳领域具有潜在的应用价值。

关键词(KeyWords)： 玫瑰考克氏菌;胞外蛋白酶;酶学性质;大豆分离蛋白;氨基酸

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31000808);; 黑龙江省青年学术骨干(1252G009);; 中国博士后基金特别资助(201104383)

作者(Author): 李娟娟,陈红,郭力强,李春秋,刘懋,冯镇

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