螺旋藻蛋白酶解工艺的优化及螺旋藻肽的制备Optimization of Proteolysis Technology of Spirulina platensis and Preparation of Spirulina platensis Peptides
孙媛媛,郑雪君,崔春,李龄佳,许立锵
摘要(Abstract)：
利用碱性蛋白酶37071对螺旋藻蛋白进行了酶解,探究了酶解pH、酶解时间以及热处理对酶解效率的影响,得到螺旋藻蛋白酶解的最佳工艺条件为:酶解pH 8,酶解时间24 h,热处理100℃。此时,螺旋藻蛋白回收率达到51.78%,水解度为13.16%。在此最优工艺条件下,制备得到了螺旋藻肽,并对该肽的蛋白含量、氨氮含量、氨基酸组成、总糖含量、分子量分布以及金属元素含量进行了测定。
关键词(KeyWords)： 螺旋藻蛋白;碱性蛋白酶;工艺;螺旋藻肽
基金项目(Foundation): 广东省科技计划项目(2017A010105002);; 广州市科技计划项目(201604020067);; 中央高校基本业务费项目(2017ZD093)
作者(Author): 孙媛媛,郑雪君,崔春,李龄佳,许立锵
参考文献(References)：
- [1]Claudia A O,Julio C de C,Gilberto V de M,et al.Functional properties and health benefits of bioactive peptides derived from Spirulina:a review[J].Food Reviews International,2018,34(1):34-51.
- [2]Aline M P,Cristiane R L,Jorge A V C.High protein ingredients of microalgal origin:obtainment and functional properties[J].Innovative Food Science and Emerging Technologies,2018,47:187-194.
- [3]陈丽娜,温宇旗,韩国庆,等.生物活性肽制备工艺的研究进展[J].农产品加工,2018(17):57-62.
- [4]胡双飞,张学武,范晓丹.超声耦合亚临界水提取螺旋藻粗蛋白及其抗氧化活性研究[J].现代食品科技,2018,34(9):150-157.
- [5]田其英.螺旋藻脱除腥味的研究[J].畜牧与饲料科学,2009,30(4):78-79.
- [6]Pearce K N,Kinsella J E.Emulsifying properties of proteins:evaluation of a turbidimetric technique[J].Journal of Agricultural and Food Chemistry,1978,26(3):716-723.
- [7]Tang C H,Chen L,Foegeding E A.Mechanical and water-holding properties and microstructures of soy protein isolate emulsion gels induced by CaCl2,glucono-δ-lactone (GDL),and transglutaminase:influence of thermal treatments before and/or after emulsification[J].Journal of Agricultural & Food Chemistry,2011,59(8):4071-4074.
- [8]崔春.食物蛋白质控制酶解技术[M].北京:中国轻工业出版社,2018:72.
- [9]Margit D A,Magni M,Leif P,et al.Chemical and sensory characterization of hydrolyzed vegetable protein,a savory flavoring[J].Journal of Agriculture and Food Chemistry,1998,46:481-489.
- [10]Celekli A,Yavuzatmaca M.Predictive modeling of biomass production by Spirulina platensis as function of nitrate and NaCl concentrations[J].Bioresource Technology,2009,100(5):1847-1851.
- [11]Ciferri O.Spirulina,the edible organism[J].Microbiological Reviews,1984,47(4):551-578.