于文平,张亚豪,吴正云,宫尾茂雄,张文学

• 1:四川大学轻纺与食品学院
• 2:东京家政大学家政学部
• 3:四川大学锦江学院白酒学院

摘要(Abstract)：

采用PCR-DGGE和qPCR技术检测四川工业青菜泡菜发酵过程中微生物群落组成。细菌DGGE条带表明,工业青菜泡菜中含有2个门,分别是厚壁菌门(Firmicutes)和变形菌门(Proteobacteria);共有12个细菌属,包括乳杆菌属(Lactobacillus)、盐单胞菌属(Halomonas)和假单胞菌属(Pseudomonas)等,其中乳杆菌属的相对丰度最高,qPCR检测其含量为10~7～10~(11) copies/mL。真菌DGGE条带表明,工业青菜泡菜的真菌属有5个,分别是德巴利氏酵母属(Debaryomyces)、假丝酵母属(Candida)、酵母属(Saccharomyces)、柯达酵母属(Kodamaea)、Cystofilobasidium,其中德巴利氏酵母属的相对丰度最高。

关键词(KeyWords)： 工业泡菜;细菌群落;真菌群落;DGGE;qPCR

Abstract:

Keywords:

基金项目(Foundation): 四川省科技计划项目(2016NZ0007)

作者(Author): 于文平,张亚豪,吴正云,宫尾茂雄,张文学

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