李楠,陈俊霖,顾双,王向阳,练志峰,付蕾,程周周

• 1:浙江工商大学食品与生物工程学院

摘要(Abstract)：

桔霉素是一种真菌毒素,红曲等一些发酵食品常含有桔霉素。为了了解食品桔霉素的含量,选用桔青霉免疫亲和柱纯化酱油、黄酒、红曲粉。增加桔霉素免疫亲和柱的洗脱液用量,减少HPLC进样量来优化检测方法,用HPLC-FLD检测桔霉素含量。结果发现其有较好的去除杂质效果,红曲粉的检测限和定量限分别为16,54μg/kg,测定标准偏差(RSD)为0.823%。红曲粉、黄酒、酱油的平均回收率分别为84.72%,86.22%,80.34%。测得市售酱油、黄酒、红曲粉的桔霉素的含量分别为2.453~2.517μg/mL,0.629~1.907μg/mL和0.644μg/g。

关键词(KeyWords)： 桔霉素;免疫亲和柱;高效液相荧光测定

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金项目(LY15C200005)

作者(Author): 李楠,陈俊霖,顾双,王向阳,练志峰,付蕾,程周周

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