不同发酵条件下萝卜泡菜生物胺变化规律研究Study on the Changes of Biogenic Amines in Radish Pickles Under Different Fermentation Conditions
陈露,马良,谭红霞,朱文优,曾鹏,尹礼国
摘要(Abstract):
旨在分析自然发酵下,不同发酵时间、温度和盐浓度的萝卜泡菜中生物胺含量变化规律以及不同发酵时间下泡菜感官品质的变化情况。采用家庭自制泡菜的制作工艺,在不同发酵条件下进行泡菜制作。对萝卜泡菜样品进行捣碎、研磨、提取、衍生等处理后,采用高效液相色谱法测定生物胺含量。结果表明,随着发酵时间的增加,萝卜泡菜中总生物胺含量先增加再缓慢减少。其中,在第11天时,含量最高(281.28 mg/kg)。在相同发酵时间下,腐胺的含量最高。当发酵温度为20℃时,泡菜中总生物胺含量最高(105.58 mg/kg),25℃时总生物胺含量最低(81.32 mg/kg)。随着盐浓度的增加,生物胺含量逐渐降低。此外,发酵7 d的泡菜感官品质最好。通过研究,可为不同发酵条件下泡菜中生物胺变化规律的研究以及品质调控提供参考。
关键词(KeyWords): 萝卜泡菜;生物胺;发酵时间;发酵温度;盐浓度
基金项目(Foundation): 固态发酵资源利用四川省重点实验室2020年度开放基金项目(2020GTJ007);; 川菜发展研究中心2021年度科研项目(CC21Z12);; 宜宾学院2021年校级科研项目(2021PY06)
作者(Author): 陈露,马良,谭红霞,朱文优,曾鹏,尹礼国
参考文献(References):
- [1]陈露,尹礼国,朱文优,等.泡菜中生物胺污染及控制方法研究进展[J].食品与发酵工业,2022(11):303-309,323.
- [2]张楠笛,祝林,许琴,等.食窦魏斯氏菌协同植物乳杆菌改善四川泡菜风味[J].食品科学,2020,41(14):102-108.
- [3]CHEN A J,LUO W,PENG Y T,et al.Quality and microbial flora changes of radish paocai during multiple fermentation rounds[J].Food Control,2019,106:106733-106745.
- [4]张林,罗陶,张华,等.四川泡菜分类归属及定义的分析与建议[J].食品安全质量检测学报,2019,10(5):1250-1253.
- [5]XIANG W L,ZHANG N D,LU Y,et al.Effect of Weissella cibaria co-inoculation on the quality of Sichuan pickle fermented by Lactobacillus plantarum[J].LWT-Food Science and Technology,2020,121:108975-108981.
- [6]EL SHEIKHA A F,HU D M.Molecular techniques reveal more secrets of fermented foods[J].Critical Reviews in Food Science and Nutrition,2020,60(1):11-32.
- [7]SANLIER N,GOKCEN B B,SEZGIN A C.Health benefits of fermented foods[J].Critical Reviews in Food Science and Nutrition,2019,59(3):506-527.
- [8]DAS G,PARAMITHIOTIS S,SIVAMARUTHI B S,et al.Traditional fermented foods with anti-aging effect:a concentric review[J].Food Research International,2020,134:1-12.
- [9]MUN E G,SOHN H S,KIM M S,et al.Antihypertensive effect of Ganjang (traditional Korean soy sauce) on Sprague-Dawley rats[J].Nutrition Research and Practice,2017,11(5):388-395.
- [10]PARK Y K,LEE J H,MAH J H.Occurrence and reduction of biogenic amines in kimchi and Korean fermented seafood products[J].Foods,2019,8(11):547-551.
- [11]MARCO M L,HEENEY D,BINDA S,et al.Health benefits of fermented foods:microbiota and beyond[J].Current Opinion in Biotechnology,2017,44:94-102.
- [12]ANAL A K,PERPETUINI G,PETCHKONGKAEW A,et al.Food safety risks in traditional fermented food from South-East Asia[J].Food Control,2020,109:106922-106930.
- [13]LY D L,MAYRHOFER S,SCHMIDT J M,et al.Biogenic amine contents and microbial characteristics of cambodian fermented foods[J].Foods,2020,9(2):198-216.
- [14]DEL RIO B,REDRUELLO B,LINARES D M.The biogenic amines putrescine and cadaverine show in vitro cytotoxicity at concentrations that can be found in foods[J].Scientific Reports,2019,9:120-126.
- [15]WANG Z X,SHAO Y Y.Effects of microbial diversity on nitrite concentration in paocai,a naturally fermented cabbage product from China[J].Food Microbiology,2018,72:185-192.
- [16]LIU L B,DU P,ZHANG G F,et al.Residual nitrite and biogenic amines of traditional northeast sauerkraut in China[J].International Journal of Food Properties,2017,20(11):2448-2455.
- [17]FONG F L Y,LAM K Y,LAU C S,et al.Reduction in biogenic amines in douchi fermented by probiotic bacteria[J].PLoS One,2020,15(3):230916-230925.
- [18]张杰,赵志峰,王佐军,等.四川泡菜菌相构成、风味与质量安全性研究进展[J].中国调味品,2021,46(10):178-182,197.
- [19]唐垚,唐小曼,明建英,等.四川泡菜产生物胺细菌的筛选及产胺能力验证[J].中国调味品,2019,44(7):81-84.
- [20]YANG J X,CAO J L,XU H Y,et al.Bacterial diversity and community structure in Chongqing radish paocai brines revealed using PacBio single-molecule real-time sequencing technology[J].Journal of the Science of Food and Agriculture,2018,98(9):3234-3245.
- [21]迟雪梅,张庆芳,迟乃玉.发酵蔬菜安全性的研究进展[J].中国酿造,2018,37(8):5-8.
- [22]GARDINI F,OZOGUL Y,SUZZI G,et al.Technological factors affecting biogenic amine content in foods:a review[J].Frontiers in Microbiology,2016,7:1218-1227.
- [23]王奇,王传明,周雨,等.泡菜中微生物菌群的研究进展[J].中国调味品,2021,46(9):197-200.
- [24]汪冬冬,唐垚,伍亚龙,等.泡菜细菌多样性和风味成分研究进展[J].食品与发酵工业,2021,47(21):296-302.
- [25]李彬彬,徐晔,牛淑慧,等.食品中生物胺含量及生物胺氧化酶的研究进展[J].食品科学,2019,40(1):341-347.
- [26]SANTOSBUELGA C,PENAEGIDO M J,RIVASGONZALO J C.Changes in tyramine during chorizo-sausage ripening[J].Journal of Food Science,2015,52(1):518-519.
- [27]MENG J,YANG Q,WAN W Y,et al.Physicochemical properties and adaptability of amine-producing Enterobacteriaceae isolated from traditional Chinese fermented fish (Suan yu)[J].Food Chemistry,2022,369:130885-130897.
- [28]AHANGARI H,KURBANOGLU S,EHSANI A,et al.Latest trends for biogenic amines detection in foods:enzymatic biosensors and nanozymes applications[J].Trends in Food Science and Technology,2021,112:75-87.
- [29]CVETKOVIC B R,PEZO L L,TASIC T,et al.The optimization of traditional fermentation process of white cabbage (in relation to biogenic amines and polyamines content and microbiological profile)[J].Food Chemistry,2015,168:471-477.
- [30]LYU J,LI C C,LI S J,et al.Effects of temperature on microbial succession and quality of sour meat during fermentation[J].LWT-Food Science and Technology,2019,114:108391-108398.
- [31]LI D W,LIANG J J,SHI R Q,et al.Occurrence of biogenic amines in sufu obtained from Chinese market[J].Food Science and Biotechnology,2019,28(2):319-327.
- [32]GUO J,LUO W,FAN J,et al.Co-inoculation of Staphylococcus piscifermentans and salt-tolerant yeasts inhibited biogenic amines formation during soy sauce fermentation[J].Food Research International,2020,137:109436-109442.
- [33]白雪菲,金刚,刘思,等.低温条件下希氏乳杆菌Q19苹果酸-乳酸发酵特性及其对葡萄酒香气成分的影响[J].食品科学,2020,41(18):146-152.
- [34]LEE J S,JIN Y H,PAWLUK A M,et al.Reduction in biogenic amine content in Baechu (Napa cabbage) kimchi by biogenic amine-degrading lactic acid bacteria[J].Microorganisms,2021,9(12):2570-2586.
- [35]LI S Y,DU X,FENG L,et al.The microbial community,biogenic amines content of soybean paste,and the degradation of biogenic amines by Lactobacillus plantarum HM24[J].Food Science and Nutrition,2021,9(12):6458-6470.
- [36]王浩文,王传明,叶丹,等.四川泡菜特征风味研究进展[J].中国调味品,2021,46(10):198-200.