丹贝酱的抗氧化活性及风味成分分析Analysis of Antioxidant Activity and Flavor Components of Tempeh Paste
李梦茹,杨文清,李西西,李佳莹,赵雪如,付玮琦,李风娟
摘要(Abstract):
丹贝是一种以大豆为原料经根霉等发酵而制得的产品,文章采用后发酵工艺对鲜丹贝进行进一步加工得到衍生产品丹贝酱,考察了丹贝酱在后发酵过程中风味物质、抗氧化能力、总酚含量、总黄酮含量和游离氨基含量的变化。结果表明,丹贝经后发酵72 h后所得丹贝酱样品水提物呈现良好的抗氧化活性,DPPH、ABTS自由基清除能力、Fe~(3+)还原能力分别为1.526,1.609,0.983 mg TE/g DW,总酚及总黄酮含量分别为1.811 mg GAE/g DW和0.113 mg CE/g DW;风味成分分析发现,后发酵72 h丹贝酱样品中1-辛烯-3-酮、3-辛酮、1-辛烯-3-醇、壬醛和2-正戊基呋喃的气味活性值(OAV)较高,可被视为主要香气成分,将为具有良好功能活性及感官品质的丹贝酱及其他丹贝衍生产品的开发提供基础理论参考。
关键词(KeyWords): 后发酵;丹贝酱;抗氧化能力;酚类物质;风味
基金项目(Foundation): 天津市自然科学基金项目(16JCYBJC23200)
作者(Author): 李梦茹,杨文清,李西西,李佳莹,赵雪如,付玮琦,李风娟
参考文献(References):
- [1]丁一.添加谷物的新型丹贝研发及其抗氧化活性评价[D].南京:南京农业大学,2012.
- [2]WOLKERS-ROOIJACKERS J C M,ENDIKA M F,SMID E J.Enhancing vitamin B12 in lupin tempeh by in situ fortification[J].LWT-Food Science and Technology,2018,96:513-518.
- [3]SOKA S,SUWANTO A,RUSMANA I,et al.Analysis of intestinal mucosal immunoglobulin A in Sprague Dawley rats supplemented with tempeh[J].Hayati Journal of Biosciences,2015,22(1):48-52.
- [4]NAKAJIMA N,NOZAKI N,ISHIHARA K,et al.Analysis of isoflavone content in tempeh,a fermented soybean,and preparation of a new isoflavone-enriched tempeh[J].Journal of Bioscience and Bioengineering,2005,100(6):685-687.
- [5]黄璐.混合菌株发酵及后熟对丹贝品质和功能特性的影响[D].南京:南京农业大学,2018.
- [6]王迪,王智荣,陈湑慧,等.不同后发酵温度下曲霉型豆豉的氨基酸态氮生成动力学及品质变化研究[J].食品与发酵工业,2021,47(9):91-99.
- [7]BRAND-WILLIAMS W,CUVELIER M E,BERSET C.Use of afree radical method to evaluate antioxidant activity[J].LWT-Food Science and Technology,1995,28:25-30.
- [8]高一芳,刘雪婷,闫仲丽,等.牡丹花水提物对肾素和ACE的双重抑制作用[J].现代食品科技,2021,37(2):114-119,260.
- [9]张嵘,相启森,王利敏,等.植物多酚在肉及肉制品中的应用研究进展[J].中国调味品,2021,46(10):183-187.
- [10]BAGHERI N,LAWATI H A J,HASSANZADEH J.Simultaneous determination of total phenolic acids and total flavonoids in tea and honey samples using an integrated lab on a chip device[J].Food Chemistry,2021,342:128338.
- [11]吕明帅,赵博,孙文玉,等.富硒黑木耳多糖的理化性质及抗氧化活性研究[J].中国调味品,2021,46(6):54-59.
- [12]JOHN J A,SHAHIDI F F.Phenolic compounds and antioxidant activity of Brazil nut (Bertholletia excelsa)[J].Journal of Functional Foods,2010,2(3):196-209.
- [13]时培宁,谭笑,李莹,等.功能性调味黑蒜液加工中品质变化研究[J].中国调味品,2021,46(10):47-53.
- [14]商全乐,白迎秋,彭一腾.陈醋多酚提取物抗氧化活性及抗疲劳作用研究[J].中国调味品,2020,45(7):168-171.
- [15]KULIGOWSKI M,PAWLOWSKA K,JASINSKA-KULIGOWSKA I,et al.Isoflavone composition,polyphenols content and antioxidative activity of soybean seeds during tempeh fermentation[J].CyTA-Journal of Food,2016,15(1):27-33.
- [16]王丹丹.黑豆丹贝的研制及生理活性物质的研究[D].太原:山西农业大学,2013.
- [17]LEE I H,HUNG Y H,CHOU C C.Solid-state fermentation with fungi to enhance the antioxidative activity,total phenolic and anthocyanin contents of black bean[J].International Journal of Food Microbiology,2008,121(2):150-156.
- [18]JANICANIC H E,TOMIC J,TORBICA A,et al.Content of free amino groups during postharvest wheat and flour maturation in relation to gluten quality[J].Food Chemistry,2014,164:158-165.
- [19]YANG Y,WANG B,FU Y,et al.HS-GC-IMS with PCA to analyze volatile flavor compounds across different production stages of fermented soybean whey tofu[J].Food Chemistry,2021,346:128880.
- [20]WANG M Q,MA W J,SHI J,et al.Characterization of the key aroma compounds in Longjing tea using stir bar sorptive extraction (SBSE) combined with gas chromatography-mass spectrometry (GC-MS),gas chromatography-olfactometry (GC-O),odor activity value (OAV),and aroma recombination[J].Food Research International,2020,130:108908.
- [21]FENG X M,LARSEN T O,SCHNURER J,et al.Production of volatile compounds by Rhizopus oligosporus during soybean and barley tempeh fermentation[J].International Journal Food Microbiology,2007,113(2):133-141.
- [22]KROGERUS K,GIBSON B R.Influence take of valine and other amino acids on total diacetyl and 2,3-pentanedione levels during fermentation of brewer's wort[J].Applied Microbiology and Biotechnology,2013,97(15):6919-6930.