郑莹,李守辉,丁成芳,王旭锋,文子艳,郭庆彬,王昌禄,丁文涛

• 1:天津科技大学食品科学与工程学院
• 2:天津科技大学省部共建食品营养与安全国家重点实验室

摘要(Abstract)：

为提高紫色红曲霉M9(M.purpureus M9)的胞外多糖(exopolysaccharides, EPS)产量，发酵时加入非离子表面活性剂吐温80(Tween-80),探究其对EPS产量和组成的影响。基于单因素试验和响应面试验，分析Tween-80添加量、添加时间和摇瓶发酵时间对EPS产量的影响，解析EPS的分子量和单糖组成，探究在最优条件下添加Tween-80对菌丝体形态的影响。结果表明，在Tween-80添加量为30 g/L、添加时间为发酵4 d后、摇瓶发酵时间为9 d时，试验组(添加Tween-80,EG)的EPS产量为(3.53±0.01) g/L,产量提高213.60%,相比于对照组(未添加Tween-80,CK)的单糖组成，半乳糖含量增加36.72%,葡萄糖含量减少24.62%。添加Tween-80使紫色红曲霉M9的菌丝体直径变小、表面皱缩、菌丝数量减少、荧光强度减弱。添加Tween-80进行摇瓶发酵可以增加紫色红曲霉M9胞外多糖产量并改变菌丝体形态，该结果为胞外多糖的工业化生产提供了参考。

关键词(KeyWords)： 紫色红曲霉;胞外多糖;非离子表面活性剂;响应面法

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(32272277);; 天津市研究生科研创新项目(2019YJSB011)

作者(Author): 郑莹,李守辉,丁成芳,王旭锋,文子艳,郭庆彬,王昌禄,丁文涛

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