咸味肽的制备技术研究进展Research Progress on the Preparation Technology of Salty Peptides
张康逸,屈凌波,温青玉,张雨,董彩虹
摘要(Abstract):
咸味肽在食品调料中起着至关重要的作用,特别是针对需低钠食品的特殊人群,存在很大的潜在利用价值。因此,对咸味肽的制备开发成为研究热点。文章分别从咸味肽研究必要性及结构特点、咸味肽分类、咸味肽受体与机制、咸味肽构效关系、咸味肽制备技术、分离纯化鉴定技术、咸味肽应用以及咸味肽的未来发展趋势等方面对其进行总结归纳,以期为促进咸味肽的深入机理研究及产品开发奠定理论依据。
关键词(KeyWords): 咸味肽;受体机制;构效关系;制备技术;分离纯化
基金项目(Foundation): 河南省重大公益专项(201300110300);; 河南省科技攻关项目(222102110256);; 河南省农业科学院杰出青年科技基金(2020JQ04);河南省农业科学院科技创新创意项目(豫财科[2020]36号2060302);河南省农业科学院优秀青年科技基金(2022YQ30);河南省农业科学院科技创新团队(2022TD24)
作者(Author): 张康逸,屈凌波,温青玉,张雨,董彩虹
参考文献(References):
- [1]黄文垒,毕继才,闫寒.咸味肽研究进展[J].食品工业,2020,41(10):261-264.
- [2]CAPPUCCIO F P,BEER M,STRAZZULLO P.Population dietary salt reduction and the risk of cardiovascular disease.A scientific statement from the European salt action network[J].Nutrition,Metabolism,and Cardiovascular Diseases,2018,29(2):107-114.
- [3]张杰,赵志峰,郝罗,等.减盐策略及低钠盐研究进展[J].中国调味品,2021,46(3):179-184.
- [4]KESKA P,STADNIK J.Taste-active peptides and amino acids of pork meat as components of dry-cured meat products:an in-silico study[J].Journal of Sensory Studies,2017,32(6):12301.
- [5]TAKAHASHI H,RIKIMARU K,KIYOHARA R,et al.Effect of arachidonic acid-enriched oil diet supplementation on the taste of broiler meat[J].Animal Bioscience,2012,25(6):845-851.
- [6]张雅玮,郭秀云,彭增起.食盐替代物研究进展[J].肉类研究,2011,25(2):36-38.
- [7]周雪松,赵谋明.肽的呈味功能研究[J].中国调味品,2005(6):38-42.
- [8]TADA M,SHINODA I,OKAI H.L-Ornithylaurine,a new salty peptide[J].Journal of Agricultural and Food Chemistry,2002,32(5):992-996.
- [9]ZHU X L,WATANABE K,SHIRAISHI K,et al.Identification of ACE-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell monolayers[J].Peptides,2008,29(3):338-344.
- [10]彭增起,张雅玮,郭秀云.一种多肽食盐替代物及其制备方法:中国,CN102224921A[P].2011-10-26.
- [11]ZHENG Y Y,TANG L,YU M G,et al.Fractionation and identification of salty peptides from yeast extract[J].Journal of Food Science and Technology,2020,58(3):1199-1208.
- [12]武彦文,欧阳杰.氨基酸和肽在食品中的呈味作用[J].中国调味品,2001(1):19-22.
- [13]王书佩.α-L-Asp-Asp和α-L-Lys-Lys的固相合成工艺研究[D].新乡:河南师范大学,2013.
- [14]王欣,安灿,陈美龄,等.酶水解哈氏仿对虾蛋白提高咸味的研究[J].中国调味品,2017,42(5):12-16.
- [15]吴迪,OMODO J,MAGAWA G,等.响应面分析优化酶水解小黄鱼(Larimichthys polyactis)蛋白产生的咸味肽及其风味度[J].中国食品添加剂,2017(9):68-74.
- [16]吴阳.双孢菇中浓厚感呈味肽与基本味感相互作用的研究[D].上海:上海应用技术大学,2019.
- [17]OKA Y,BUTNARU M,BUCHHOLTZ L V,et al.High salt recruits aversive taste pathways[J].Nature,2013,494(7438):472-475.
- [18]LIMAN E R.Salty taste:from transduction to transmitter release,hold the calcium[J].Neuron,2020,106(5):709-711.
- [19]TAMURA M,NAKATSUKA T,TADA M,et al.The relationship between taste and primary structure of “delicious peptide”(Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala) from beef soup[J].Bioscience,Biotechnology,and Biochemistry,2014,53(2):319-325.
- [20]SCHINDLER A,DUNKEL A,ST?HLER F,et al.Discovery of salt taste enhancing arginyl dipeptides in protein digests and fermented fish sauces by means of a sensomics approach[J].Journal of Agricultural and Food Chemistry,2011,59(23):12578-12588.
- [21]HARTH L,KRAH U,LINKE D,et al.Salt taste enhancing L-arginyl dipeptides from casein and lysozyme released by peptidases of basidiomycota[J].Journal of Agricultural and Food Chemistry,2018,66(10):2344-2353.
- [22]党亚丽,张中健,闫小伟,等.巴马火腿酶解物中呈味肽的分离纯化及其结构研究[J].食品科学,2010,31(13):127-131.
- [23]张铭霞,陈思羽,李露,等.食品中呈味肽类组分研究进展[J].中国食品学报,2016,16(2):209-217.
- [24]OHTA S,SAITOH C,IWASAKI H,et al.Method of enhancing salty taste,salty taste enhancer,salty taste seasoning agent and salty taste-enhanced foods:US,US06974597B2[P].2005-12-13.
- [25]SELAMASSAKUL O,LAOHAKUNJIT N,KERDCHOECHUEN O,et al.Bioactive peptides from brown rice protein hydrolyzed by bromelain:relationship between biofunctional activities and flavor characteristics[J].Journal of Food Science,2020,85(3):707-717.
- [26]SONG X L,SHI Z H,LI X F,et al.Fate of proteins of waste activated sludge during thermal alkali pretreatment in terms of sludge protein recovery[J].Frontiers of Environmental Science & Engineering,2019,13(2):25.
- [27]AYU S Z,RABETA M S.Enzymatic hydrolysis as an approach to produce alternative protein from cephalopods ink powder:a short review[J].Journal of Food Science,2020,4(5):1383-1390.
- [28]EBAID R,WANG H C,SHA C,et al.Recent trends in hyperthermophilic enzymes production and future perspectives for biofuel industry:a critical review[J].Journal of Cleaner Production,2019,238(C):117925.
- [29]CHAI K F,VOO A Y H,CHEN W N.Bioactive peptides from food fermentation:a comprehensive review of their sources,bioactivities,applications,and future development[J].Comprehensive Reviews in Food Science and Food Safety,2020,19(6):3825-3885.
- [30]崔春,赵谋明,曾晓房,等.酸法和酶法水解植物蛋白的差异及原因探讨[J].中国调味品,2006(7):9-13.
- [31]ZHAO Y Q,ZHAO X,SUN D X,et al.Two-stage selective enzymatic hydrolysis generates protein hydrolysates rich in Asn-Pro and Ala-His for enhancing taste attributes of soy sauce[J].Food Chemistry,2021,345:128803.
- [32]LI L J,TAN W S,LI W J,et al.Citrus taste modification potentials by genetic engineering[J].International Journal of Molecular Sciences,2019,20(24):6194.
- [33]BABINI E,TANEYO-SAA D L,TASSONI A,et al.Microbial fermentation of industrial rice-starch byproduct as valuable source of peptide fractions with health-related activity[J].Microorganisms,2020,8(7):986.
- [34]李长平,杨俊,黄河,等.源于凡纳滨对虾血蓝蛋白化学合成肽段的抗黑曲霉活性[J].中国水产科学,2018,25(1):189-194.
- [35]YANG J H,WANG L,YAO C C,et al.Site-specific incorporation of multiple thioamide substitutions into a peptide backbone via solid phase peptide synthesis[J].The Journal of Organic Chemistry,2020,85(3):1484-1494.
- [36]SHAER D A,ALBERICIO F,TORRE B.Solid-phase synthesis of peptides containing 1-hydroxypyridine-2-one (1,2-HOPO)[J].Tetrahedron Letters,2020,61(37):152299.
- [37]YEO J,PEEVA L,CHUNG S,et al.Liquid phase peptide synthesis via one-pot nanostar sieving (PEPSTAR)[J].Angewandte Chemie,2021,60(14):7786-7795.
- [38]FAMUWAGUN A A,ALASHI A M,GBADAMOSI O S,et al.Antioxidant and enzymes inhibitory properties of Amaranth leaf protein hydrolyzates and ultrafiltration peptide fractions[J].Journal of Food Biochemistry,2021,45(3):13396.
- [39]KAPEL R,KLINGENBERG F,FRAMBOISIER X,et al.An original use of size exclusion-HPLC for predicting the performances of batch ultrafiltration implemented to enrich a complex protein hydrolysate in a targeted bioactive peptide[J].Journal of Membrane Science,2011,383(1):26-34.
- [40]LIU P,HUANG M G,SONG S Q,et al.Sensory characteristics and antioxidant activities of Maillard reaction products from soy protein hydrolysates with different molecular weight distribution[J].Food and Bioprocess Technology,2012(5):1775-1789.
- [41]ZHANG J N,ZHAO M M,SU G W,et al.Identification and taste characteristics of novel umami and umami-enhancing peptides separated from peanut protein isolate hydrolysate by consecutive chromatography and UPLC-ESI-QTOF-MS/MS[J].Food Chemistry,2018,278:674-682.
- [42]张顺亮,成晓瑜,乔晓玲,等.牛骨酶解产物中咸味肽组分的分离纯化及成分研究[J].食品科学,2012,33(6):29-32.
- [43]ZHAO P Y,ZARE R N,CHEN H.Absolute quantitation of oxidizable peptides by coulometric mass spectrometry[J].Journal of the American Society for Mass Spectrometry,2019,30(11):2398-2407.
- [44]ABDELHAMID H N,WU H F.A new binary matrix for specific detection of mercury(II)using matrix-assisted laser desorption ionization mass spectrometry[J].Journal of the American Society for Mass Spectrometry,2019,30(12):2617-2622.
- [45]PERU K M,THOMAS M J,LOZANO D C P,et al.Characterization of oil sands naphthenic acids by negative-ion electrospray ionization mass spectrometry:influence of acidic versus basic transfer solvent[J].Chemosphere,2019,222:1017-1024.
- [46]SENTANDREU E,FUENTE-GARCIA C,NAVARRO J L,et al.A straightforward gel-free proteomics pipeline assisted by liquid isoelectric focusing (OFFGEL) and mass spectrometry analysis to study bovine meat proteome[J].Food Science and Technology International,2021,27(2):112-122.
- [47]BENDINSKAS K G,CANNING A J,SMITH A E.Identification of proteins using four methods of matrix-assisted laser desorption ionization time-of-flight mass spectrometry[J].Journal of Chemical Education,2019,96(11):2600-2605.
- [48]李迎楠,刘文营,张顺亮,等.色谱纯化和质谱分析法研究牛骨源咸味肽[J].肉类研究,2016,30(3):25-28.
- [49]HAUSER A,POULOU E,MüLLER F,et al.Synthesis and evaluation of non-hydrolyzable phospho-lysine peptide mimics[J].Chemistry-A European Journal,2020,27(7):2326-2331.
- [50]BAKSHI K,LIYANAGE M R,VOLKIN D B,et al.Fourier transform infrared spectroscopy of peptides[J].Methods in Molecular Biology,2014,1088:255-269.
- [51]WU S Z,HE Z P,WANG Q Q,et al.Response surface optimization of enzymatic hydrolysis of peptides of Chinese pecan (Carya cathayensis) and analysis of their antioxidant capacities and structures[J].International Journal of Peptide Research and Therapeutics,2021,27:1239-1251.
- [52]迟韵,陈虎,陈留平,等.一种增咸食用盐及其制备方法:中国,CN110973580A[P].2020-04-10.
- [53]SCHINDLER A,DUNKEL A,ST?HLER F,et al.Discovery of salt taste enhancing arginyl dipeptides in protein digests and fermented fish sauces by means of a sensomics approach[J].Journal of Agricultural and Food Chemistry,2011,59(23):12578-12588.
- [54]陈瑞霞,孙思远,相悦,等.以淘汰蛋鸡为原料利用蛋白酶解技术制备咸味增强肽[J].食品与发酵工业,2020,46(1):166-171.
- [55]郑家伦,李晨,陆利霞,等.基于大豆粕酶解物美拉德反应制备咸味香精的研究[J].中国调味品,2017,42(10):4-10.