盐浓度对东北农家酱品质变化的影响Effect of Salt Concentration on the Quality of Farmhouse Fermented Soybean Paste in the Northeast China
解双瑜,毛文颖,李智,刘东傲,孙波
摘要(Abstract)：
文章研究了东北农家酱发酵过程中盐浓度对其品质变化的影响。通过控制东北农家酱的盐浓度(8%、10%、12%),在其发酵过程中(0,15,30,45,60 d)测定其品质变化，包括感官品质(滋味、气味、色泽、体态)、理化与微生物指标(总酸、氨基酸态氮、菌落总数),同时采用电子鼻对产生的风味物质种类进行了初步的分析。结果表明，盐浓度为10%的样品感官品质评分最高，其各项评分分别为35.90,25.90,17.30,9.00。电子鼻结果表明，东北农家酱在发酵过程中，会生成甲基类、醇类、醛酮类化合物，而盐浓度的增加会抑制烷烃类、醇类与氢化物类化合物的产生。酱中的总酸、氨基酸态氮与菌落总数随着盐浓度的增加呈现出下降的趋势。其中盐浓度为10%的样品的理化与微生物指标最为适宜，其各项指标分别为1.06 g/100 g, 1.68 g/100 g, 8.10 lg CFU/g。由此可知，过高或过低的盐浓度都会降低东北农家酱的品质，结合感官品质评价与理化指标可知当盐浓度为10%比较适合东北农家酱的发酵。
关键词(KeyWords)： 东北农家酱;盐浓度;感官品质;电子鼻;理化指标;微生物指标
基金项目(Foundation):
作者(Author): 解双瑜,毛文颖,李智,刘东傲,孙波
参考文献(References)：
- [1]安飞宇，姜静，武俊瑞，等.自然发酵豆酱的滋味特性与微生物多样性分析[J].中国食品学报，2020,20(7):207-215.
- [2]马晓菲.满族非物质文化遗产产业化研究[D].沈阳：沈阳建筑大学，2018.
- [3]AN F Y,LI M,ZHAO Y,et al.Metatranscriptome-based investigation of flavor-producing core microbiota in different fermentation stages of dajiang,a traditional fermented soybean paste of Northeast China[J].Food Chemistry,2021,343:128509.
- [4]KIM M J,KWAK H S,JUNG H Y,et al.Microbial communities related to sensory attributes in Korean fermented soy bean paste (doenjang)[J].Food Research International,2016,89:724-732.
- [5]LEE S M,LEE S,SINGH D,et al.Comparative evaluation of microbial diversity and metabolite profiles in doenjang,a fermented soybean paste,during the two different industrial manufacturing processes[J].Food Chemistry,2017,221:1578-1586.
- [6]YUE X Q,LI M H,LIU Y M,et al.Microbial diversity and function of soybean paste in East Asia:what we know and what we don't[J].Current Opinion in Food Science,2021,37:145-152.
- [7]KIM T W,LEE J H,KIM S E,et al.Analysis of microbial communities in doenjang,a Korean fermented soybean paste,using nested PCR-denaturing gradient gel electrophoresis[J].International Journal of Food Microbiology,2009,131(2):265-271.
- [8]INOUE Y,KATO S,SAIKUSA M,et al.Analysis of the cooked aroma and odorants that contribute to umami aftertaste of soy miso (Japanese soybean paste)[J].Food Chemistry,2016,213:521-528.
- [9]STONE H,BLEIBAUM R,THOMAS H.Sensory Evaluation Practices[M].Washington D.C.:Academic Press,2012.
- [10]CHUN B H,KIM K H,SANG E J,et al.The effect of salt concentrations on the fermentation of doenjang,a traditional Korean fermented soybean paste[J].Food Microbiology,2020,86:103329.
- [11]解双瑜，孙波，刘丽，等.减盐对东北农家酱中生物胺形成的影响[J].食品科学，2021,42(3):92-97.
- [12]CAPPUCCIO F P,CAMPBELL N R C.Population dietary salt reduction and the risk of cardiovascular disease:a commentary on recent evidence[J].Journal of Clinical Hypertension,2017,19(1):4-5.
- [13]ZANDSTRA E H,LION R,NEWSON R S.Salt reduction:moving from consumer awareness to action[J].Food Quality and Preference,2016,48:376-381.
- [14]李天一，孙波，解双瑜，等.不同贮藏条件下东北农家酱品质变化及其货架期预测[J].食品与发酵工业，2020,46(10):209-214.
- [15]吴生文，林培，叶芝红，等.特香型白酒风味轮的构建及典型感官特性描述研究[J].中国酿造，2021,40(1):148-153.
- [16]宫雪，刘宁，李二虎，等.基于电子鼻的葡萄酒感官评价模型的构建[J].中国酿造，2014,33(5):67-71.
- [17]杨进军，胡金祥，王林，等.气质联用技术结合电子鼻分析红酸汤挥发性风味差异[J].食品与发酵工业，2020,46(14):234-242.
- [18]苏可珍，罗杨合，黎小椿，等.油炸时间对荔浦芋感官评价和电子鼻分析的影响[J].食品与机械，2020,36(6):56-59,74.
- [19]李晓晗，陈慧敏，王宝怡，等.电子鼻评价不同萎凋工艺对红茶香气的影响[J].茶叶通讯，2020,47(1):102-106.
- [20]BLANCO-RODRI'GUEZ A,CAMPO F,MORALES O M,et al.Development of an electronic nose to identify and classify odours from spirits beverages[J].Chemical Engineering Transactions,2016,54:337-342.
- [21]G■BICKI J,SZULCZY■SKI B.Discrimination of selected fungi species based on their odour profile using prototypes of electronic nose instruments[J].Measurement,2018,116:307-313.
- [22]SAHA P,GHORAI S,TUDU B,et al.Feature fusion for prediction of the aflavin and the arubigin in tea using electronic tongue[J].IEEE Transactions on Instrumentation and Measurement,2017,66(7):1703-1710.
- [23]国家质量监督检验检疫总局，国家标准化管理委员会.食品中总酸的测定：GB/T 12456—2008[S].北京：中国标准出版社，2008.
- [24]国家质量监督检验检疫总局，国家标准化管理委员会.食品安全国家标准食品中氨基酸态氮的测定：GB 5009.235—2016[S].北京：中国标准出版社，2016.
- [25]国家质量监督检验检疫总局，国家标准化管理委员会.食品安全国家标准食品微生物学检验菌落总数测定：GB 4789.2—2016[S].北京：中国标准出版社，2016.
- [26]TIN H S,周斌，侯莎，等.盐分对广式高盐稀态酱油发酵微生物菌群结构的影响[J].食品与发酵工业，2022,48(1):45-54.
- [27]徐向丽.一种用于冷链物流肉类品质在线检测的电子鼻系统[J].食品工业，2020,41(3):201-203.
- [28]杨希.盐浓度对蚕豆酱发酵过程中原核微生物多样性及理化因子的影响[J].食品与发酵工业，2022,48(4):200-206.
- [29]YANG Y,NIU C T,SHAN W X,et al.Physicochemical,flavor and microbial dynamic changes during low-salt doubanjiang (broad bean paste) fermentation[J].Food Chemistry,2021,351:128454.
- [30]KIM S S,KWAK H S,KIM M J.The effect of various salinity levels on metabolomic profiles,antioxidant capacities and sensory attributes of doenjang,a fermented soybean paste[J].Food Chemistry,2020,328:127176.