广西武宣地区红糟酸微生物多样性分析Analysis of Microbial Diversity of Red Vinasse Acid in Wuxuan Area of Guangxi
李洋,罗佳沂,林凤,毛瑞丰,李凯
摘要(Abstract):
该研究的目的是了解广西红糟酸发酵过程中微生物的动态变化以及与成品微生物群落的差异。基于Illumina MiSeq高通量测序技术对来自武宣县的7个发酵过程样和4个成品样进行细菌和真菌多样性分析,解析样品中微生物群落动态变化规律。结果表明,过程样的Chao值、Shannon指数和Simpson指数与成品样相比均有明显变化。伯克霍尔德菌(Burkholderia)和克雷伯氏菌(Klebsiella)是整个发酵过程中的优势菌,前者相对丰度在种子中可达60.97%,后者相对丰度可达36.62%。乳酸菌相对丰度在第6天大幅增加,相对丰度为14.29%;而醋酸杆菌属(Acetobacter)、克雷伯氏菌(Klebsiella)和片球菌属(Pediococcus)在成品中的相对丰度占比较高,相对丰度可达为55.88%、80.52%和25.8%;红曲霉(Monascus)是真菌中主要优势菌属,相对丰度可达98.96%。该研究结果对红糟酸的微生物组成和演替规律提供了一定理论基础。
关键词(KeyWords): 红糟酸;高通量;微生物;多样性;变化规律
基金项目(Foundation): 广西重点研发计划项目(2018AB45017)
作者(Author): 李洋,罗佳沂,林凤,毛瑞丰,李凯
参考文献(References):
- [1]于兰.红糟酸的制作工艺[J].中国调味品,1997(3):21-22.
- [2]张斌,王晓闻.基于高通量测序技术鉴定酸浆水中的微生物[J].中国调味品,2020,45(4):77-80.
- [3]贾晶晶,易洋,韩睿媛,等.高通量测序技术分析腌渍白菜液中原核微生物群落结构[J].中国调味品,2020,45(12):87-90.
- [4]沈琦,郝雅荞,徐潇航,等.基于高通量测序技术的盐地碱蓬根际细菌群落多样性分析[J].浙江理工大学学报(自然科学版),2020,43(5):671-677.
- [5]CHEN G,YU W H,ZHANG Q S,et al.Research of Sichuan Paocai and lactic acid bacteria[J].Advance Journal of Food Science and Technology,2014,6(1):1-5.
- [6]KUMAR S,STECHER G,TAMURA K.Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J].Molecular Biology and Evolution,2016,33(7):1870-1874.
- [7]MORILLO J A,AGUILERA M,ANTIZAR-LADISLAO B,et al.Molecular microbial and chemical investigation of the bioremediation of two-phase olive mill waste using laboratory-scale bioreactors[J].Applied Microbiology and Biotechnology,2008,79(2):309-317.
- [8]KIMURA M.A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J].Journal of Molecular Evolution,1980,16(2):111-120.
- [9]SAITOU N,NEI M.The neighbor-joining method:a new method for reconstructing phylogenetic trees[J].Molecular Biology and Evolution,1987(4):406-425.
- [10]FELSENSTEIN J.Confidence limits on phylogenies:an approach using the bootstrap[J].International Journal of Organic Evolution,1985,39(4):783-791.
- [11]沈毅,陈波,王西,等.酱香型郎酒高温大曲、酒醅和窖泥中细菌群落结构分析[J].中国酿造,2020,39(2):89-93.
- [12]CAPORASO J,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Method,2010,7(5):335-336.
- [13]贾晶晶,易洋,韩睿媛,等.高通量测序技术分析腌渍白菜液中原核微生物群落结构[J].中国调味品,2020,45(12):87-90
- [14]MORYA R,SALVACHUA D.Burkholderia:an untapped but promising bacterial genus for the conversion of aromatic compounds[J].Trends in Biotechnology,2020,38(9):13.
- [15]ZHANG L,XU D,WANG F,et al.Antifungal activity of Burkholderia sp.HD05 against Saprolegnia sp.by 2-pyrrolidone-5-carboxylic acid[J].Aquaculture,2019,511(15):634198.
- [16]吉莉莉,魏艳,何丹,等.发酵香肠中分离纯化的三株乳酸菌产酸特性研究[J].中国调味品,2020,45(2):36-39.
- [17]SWANSON K S,GIBSON G R,HUTKINS R,et al.The international scientific association for probiotics and prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics[J].Gastroenterology & Hepatology,2020,17(11):687-701.
- [18]袁飞,徐宝梁,陈颖,等.阪崎肠杆菌生长特性及耐热耐酸碱性分析[J].中国公共卫生,2008,24(12):1475-1476.
- [19]熊涛,李军波,彭飞.食盐质量浓度对传统自然发酵圆白菜的菌系结构和代谢的影响[J].食品科学,2015,36(11):172-176.
- [20]WU R,WANG L P,WANG J C,et al.Isolation and preliminary probiotic selection of lactobacilli from koumiss in Inner Mongolia[J].Journal of Basic Microbiology,2009,49(3):318-326.
- [21]MADRAZO A L,CAMPOS M R S.Review of antimicrobial peptides as promoters of food safety:limitations and possibilities within the food industry[J].Journal of Food Safety,2020,40(6):12854.
- [22]朱文淼,刘稳.乳酸菌细菌素的分子生物学研究进展[J].中国微生态学杂志,2000(2):55-57,65.
- [23]金华,吕嘉枥,罗潇.传统自然发酵蔬菜的显微表征及其细菌群落多样性分析[J].中国调味品,2021,46(3):11-17.
- [24]宁明,赵馨馨,董蕴,等.基于Illumina MiSeq测序技术不同地区辣椒酱细菌多样性分析[J].中国调味品,2020,45(2):58-63.