豆豉中产γ-氨基丁酸乳酸菌的分离鉴定Isolation and Identification of γ-Aminobutyric Acid in Lactic Acid Bacteria from Douchi
张竺英,刘昭明,黄翠姬,时粲,徐财凤,陆金池
摘要(Abstract):
为从豆豉中获得产γ-氨基丁酸的乳酸菌,采用高效液相色谱法对从广西黄姚豆豉中分离筛选得到的16株乳酸菌的产γ-氨基丁酸能力进行了研究。结果表明:菌株HY15的产γ-氨基丁酸能力较好。该菌株在含1%(W/W)谷氨酸钠的MRS发酵培养基中37℃厌氧培养48h后,发酵液中γ-氨基丁酸产量达到0.161g/L。经形态学特征、生理生化试验和16SrDNA基因鉴定,确定菌株HY15为戊糖片球菌(Pediococcus pentosaceus)。
关键词(KeyWords): γ-氨基丁酸;乳酸菌;筛选;鉴定
基金项目(Foundation):
作者(Author): 张竺英,刘昭明,黄翠姬,时粲,徐财凤,陆金池
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