基于UPLC-MS/MS分析山西陈醋与恒顺香醋的差异物质Analysis of Differential Substances Between Shanxi Mature Vinegar and Hengshun Aromatic Vinegar Based on UPLC-MS/MS
贾莹莹,秦宇,周景丽,杨春,李晨,范晓军
摘要(Abstract):
为了深入研究产地对食醋代谢产物的影响,采用超高效液相色谱-质谱技术,通过非靶向代谢组学分析,对来自山西省太原市的山西陈醋(MJA)与江苏省镇江市的恒顺香醋(HSA)的代谢产物进行了分析。结果显示,两地食醋中共鉴定出1 602种化合物,包括脂质、有机酸、有机氮化合物、有机杂环类化合物等多个类别。通过主成分分析与正交偏最小二乘判别分析可以显著区分MJA样品和HSA样品,并筛选出495种具有显著差异的物质。MJA样品中有360种差异物质的相对含量高于HSA样品,其中四甲基吡嗪、褪黑素、核黄素、吲哚等化合物对形成两地食醋的独特风味起到重要作用。代谢通路研究表明,两地食醋在有机杂环类化合物和生物碱及其衍生物的构成上存在显著性差异,这可能是造成两地食醋滋味和品质差异的决定性因素。该研究为鉴别食醋产地提供了重要的理论基础。
关键词(KeyWords): UPLC-MS/MS;差异物质;多元统计方法;代谢通路
基金项目(Foundation): 山西省重点研发计划(202102130501008);; 山西省研究生教育创新计划(2024SJ037)
作者(Author): 贾莹莹,秦宇,周景丽,杨春,李晨,范晓军
参考文献(References):
- [1]DUAN W,XIA T,ZHANG B,et al.Changes of physicochemical,bioactive compounds and antioxidant capacity during the brewing process of Zhenjiang aromatic vinegar[J].Molecules,2019,24(21):3935.
- [2]LI G Q,TAN X M,ZHANG B,et al.Hengshun aromatic vinegar improves glycolipid metabolism in type 2 diabetes mellitus via regulating PGC-1α/PGC-1β pathway[J].Frontiers in Pharmacology,2021,12:641829.
- [3]LIU J,WANG J,SHEN G Y,et al.Extraction methods and compositions of polyphenols in Shanxi aged vinegar[J].Journal of Chromatography A,2023,1705:464169.
- [4]刘然.基于疏水性DES-VALLME-HPLC测定山西陈醋中功能活性物质[D].太原:山西大学,2021.
- [5]XIA T,ZHANG B,DUAN W H,et al.Nutrients and bioactive components from vinegar:a fermented and functional food[J].Journal of Functional Foods,2020,64:103681.
- [6]李艺.山西老陈醋指纹图谱及功能成分研究[D].太原:山西大学,2018.
- [7]聂丽媛,范三红,曹林旭,等.基于UPLC-Q-TOF-MS技术以及网络药理学分析山西陈醋的小分子肽类成分及潜在作用机制[J].食品工业科技,2024,45(18):31-41.
- [8]刘兰涛,杨小兰.山西老陈醋醋泥冻干粉对高脂饮食小鼠的降血脂与抗氧化作用[J].食品科学,2015,36(9):141-145.
- [9]陈敏,朱文优,李宇薇,等.中外传统食醋风味特征及品质指标差异分析[J].中国酿造,2024,43(11):26-33.
- [10]舒静,陈轩,潘从道,等.不同品牌食醋味感特征的电子舌分析[J].中国调味品,2013,38(8):95-98,101.
- [11]刘廷锐,张超,朱文优,等.四川不同地区的熟醋及其固态发酵过程中有机酸含量变化分析[J].食品研究与开发,2020,41(3):166-172.
- [12]HAN D,YANG Y S,GUO Z T,et al.Metagenomics profiling of the microbial community and functional differences in solid-state fermentation vinegar starter (seed Pei) from different Chinese regions[J].Frontiers in Microbiology,2024,15:1389737.
- [13]斯仁达来,TUYATSETSEG J,BANZRAGCH M,等.基于UPLC-MS非靶向代谢组学分析不同年龄双峰驼肉代谢物差异[J].食品科学,2024,45(4):154-163.
- [14]徐晓敏,房城,卢芳.基于代谢组学技术的黄芩-白芍对二型糖尿病模型小鼠的作用机制[J].世界中医药,2023,18(5):600-605.
- [15]韩蓉,马燕,李圣杰,等.基于非靶向代谢组学分析不同品种新疆大果沙棘代谢物的差异[J].食品工业科技,2025,46(4):19-29.
- [16]CHEN J B,ULLAH H,ZHENG Z D X,et al.Soyasaponins reduce inflammation by downregulating MyD88 expression and suppressing the recruitments of TLR4 and MyD88 into lipid rafts[J].BMC Complementary Medicine and Therapies,2020,20(1):167.
- [17]姜天宇,肖杨,赖舒宁,等.紫苏葶对3T3-L1细胞和黄羽肉鸡脂质沉积的影响[J].中国畜牧杂志,2024,60(10):317-323.
- [18]CHEN X F,WEI C T,ZHAO J J,et al.Carnosic acid:an effective phenolic diterpenoid for prevention and management of cancers via targeting multiple signaling pathways[J].Pharmacological Research,2024,206:107288.
- [19]HASHEMZAEI M,MAMOULAKIS C,TSAROUHAS K,et al.Crocin:a fighter against inflammation and pain[J].Food and Chemical Toxicology,2020,143(1):111521.
- [20]WANG Y,LUO X,CHEN L,et al.Natural and low-caloric rebaudioside A as a substitute for dietary sugars:a comprehensive review[J].Comprehensive Reviews in Food Science and Food Safety,2023,22(1):615-642.
- [21]王家东,刘丹彤,王馨宇,等.山西老陈醋酿造过程四甲基吡嗪形成规律及稳定性研究[J].食品与发酵工业,2022,48(15):62-68.
- [22]KANG J H,GUO X D,WANG Y D,et al.Neuroprotective effects of N-acetylserotonin and its derivative[J].Neuroscience,2023,517:18-25.
- [23]THAKUR K,TOMAR S K,SINGH A K,et al.Riboflavin and health:a review of recent human research[J].Critical Reviews in Food Science and Nutrition,2017,57(17):3650-3660.
- [24]??LVAREZ-DIDUK R,GALANO A,TAN D X,et al.N-acetylserotonin and 6-hydroxymelatonin against oxidative stress:implications for the overall protection exerted by melatonin[J].The Journal of Physical Chemistry B,2015,119(27):8535-8543.
- [25]REN H Y,YUAN Q Q,LU J Y,et al.Tetrahydropiperine,a natural alkaloid with neuroprotective effects in ischemic stroke[J].Journal of Chemical Neuroanatomy,2024,136:102397.
- [26]XUE Y,LU F J,CHANG Z Z,et al.Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade[J].Nature Communications,2023,14(1):4758.
- [27]李金哥,纪艳青,张建营,等.豆豉中氨基酸代谢及其对产品品质影响研究进展[J].中国调味品,2024,49(11):213-220.
- [28]黄静,严唯玮,刘书亮,等.食醋大曲功能微生物菌群与品质解析研究进展[J].食品科学,2020,41(7):322-328.
- [29]HAQ I U,IMRAN M,NADEEM M,et al.Piperine:a review of its biological effects[J].Phytotherapy Research,2021,35(2):680-700.
- [30]NUGRAHINI A D,ISHIDA M,NAKAGAWA T,et al.Trigonelline:an alkaloid with anti-degranulation properties[J].Molecular Immunology,2020,118:201-209.
- [31]HUAN D Q,HOP N Q,SON N T.Oxymatrine:a current overview of its health benefits[J].Fitoterapia,2023,168:105565.
- [32]ZHAO Z W,SONG J J,ZHANG D K,et al.Oxysophocarpine suppresses FGFR1-overexpressed hepatocellular carcinoma growth and sensitizes the therapeutic effect of lenvatinib[J].Life Sciences,2021,264:118642.