崔博,郭金英,白周亚

• 1:河南科技大学食品与生物工程学院

摘要(Abstract)：

该研究运用多光谱法和分子对接技术分析橙皮素(hesperetin, HE)与胰蛋白酶(trypsin, TRP)相互作用猝灭类型、分子间相互作用力类型、猝灭常数、猝灭速率常数、结合常数、结合位点数、热力学参数、TRP的酶活性和HE的抗氧化活性。研究结果表明，HE与TRP之间相互作用的类型为动态静态猝灭复合型，结合比为1∶1,氢键为主要分子间作用力。HE可导致TRP的二级结构发生改变。分子对接模拟结果表明，HE-TRP体系的相互作用力除氢键外，还包括π-π单键和范德华力。酶活性和抗氧化性实验结果表明，HE与TRP的相互作用显著抑制了TRP的酶活性，显著降低了HE清除ABTS自由基和DPPH自由基的能力。结果表明，HE与TRP的结合存在多种作用力，形成的复合物能够稳定存在，从而抑制了TRP的活性。

关键词(KeyWords)： 多光谱法;分子对接;橙皮素;胰蛋白酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(32302075)

作者(Author): 崔博,郭金英,白周亚

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