酵母菌发酵生产赤藓糖醇的研究进展Research Progress on Erythritol Production by Yeast Fermentation
程磊,宗朕,陈卓静,王磊,汪超,祁勇刚,柳志杰
摘要(Abstract):
赤藓糖醇是一种新型甜味剂,具有低热量、高稳定性、食用安全性高等优点,可广泛应用于食品及日用品方面。目前赤藓糖醇最佳的工业化生产方式是微生物发酵法,其中主要使用的菌种是酵母菌,此类菌种的安全性高,生产赤藓糖醇的能力强。从菌种选育、赤藓糖醇的合成途径、基因工程和发酵工艺等方面综述酵母菌发酵生产赤藓糖醇的现状,旨在为增强酵母菌生产赤藓糖醇的能力提供参考。
关键词(KeyWords): 赤藓糖醇;微生物发酵法;酵母菌;菌种选育;基因工程;发酵工艺
基金项目(Foundation): 湖北省自然科学基金计划项目(2018CFB358);; 湖北工业大学博士启动基金(BSQD2017033)
作者(Author): 程磊,宗朕,陈卓静,王磊,汪超,祁勇刚,柳志杰
参考文献(References):
- [1]杨远志,杨海军.功能性糖醇[J].精细与专用化学品,2004,12(1):11-12.
- [2]郭传琦,葛兆强,李兆河,等.赤藓糖醇在日化领域中的应用研究[J].精细与专用化学品,2014,22(2):25-28.
- [3]车海山,陈迁乔,钟秦,等.赤藓糖醇基相变复合纤维制备及热性能[J].储能科学与技术,2017(6):644-654.
- [4]金树人.糖醇生产技术与应用[M].北京:中国轻工业出版社,2008.
- [5]祁广宾.赤藓糖醇的制备研究[D].济南:山东轻工业学院,2012.
- [6]刘媛媛,周志江,贾伟.赤藓糖醇高产菌株的研究进展[J].食品工业科技,2012(4):454-457.
- [7]肖素荣,李京东.赤藓糖醇的特性及应用[J].中国食物与营养,2008(5):26-28.
- [8]夏勇,徐彩菊,毛光明.赤藓糖醇急性毒性和遗传毒性实验研究[J].癌变·畸变·突变,2003,15(1):54-55.
- [9]王趁趁,王金伟,庞明利,等.赤藓糖醇抗龋齿功能研究进展及应用前景[J].食品安全导刊,2014(18):60-62.
- [10]Binkley W W,Wolform M I.Biosynthesis of erythritol in a new yeast[J].J Am Chem Soc,1950,72:4778-4782.
- [11]Spencer J F T,Sallans H R.Production of polyhydric alcohols by osmophilic yeasts[J].Canadian Journal of Microbiology,1956(2):72-79.
- [12]Hajny G J,Smith J H,Garver J C.Erythritol production by ayeast-like fungus[J].American Society for Microbiology,1964,12(3):240-246.
- [13]Park J,Seo B,Kim J,et al.Production of erythritol in fedbatch cultures of Trichosporon sp.[J].Journal of Fermentation&Bioengineering,1998,86(6):577-580.
- [14]Shie-Jea L,Chiou-Yen W,Jian-Ching L,et al.Screening and production of erythritol by newly isolated osmophilic yeastlike fungi[J].Process Biochemistry,2001,36(12):1249-1258.
- [15]Shie-Jea L,Chiou-Yen W,Pei-Ming W,et al.High-level production of erythritol by mutants of osmophilic Moniliellasp.[J].Process Biochemistry,2010,45:973-979.
- [16]Waldemar R,Anita R,Marta M.High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica[J].Biotechnol Lett,2009,31:377-380.
- [17]Xiaoyan L,Shun L,Jiaxing X,et al.Erythritol production by Yarrowia lipolytica mutant strain M53 enerated throughatmosphereroomtemperatureplasma mutagenesis[J].Food Sci Biotechnol,2016,26(4):979-986.
- [18]Xiaoyan L,Xinjun Y,Jinshun L,et al.A cost-effective process for the coproduction of erythritol and lipase with Yarrowia lipolytica M53from waste cooking oil[J].Food and Bioproducts Processing,2017,103:86-94.
- [19]徐虹,周基化,欧阳平凯.赤藓糖酵产生菌的筛选[J].南京化工大学学报,1995,17(Z1):14-17.
- [20]范光先,张海平,诸葛健.耐高渗酵母产赤藓糖醇的影响因素[J].无锡轻工大学学报,2001,20(2):133-136.
- [21]吴燕,杨晓伟,陆茂林.赤藓糖醇产生菌B845的形态生理特征[J].生物技术,2002,12(2):17-20.
- [22]叶娴,董海洲,侯汉学,等.赤藓糖醇高产菌株的筛选鉴定及发酵特性的初步研究[J].生物技术,2007,17(5):54-57.
- [23]王凤伟,詹晓北,郑志永,等.耐高渗产赤藓糖醇酵母的诱变筛选与鉴定[J].工业微生物,2013,43(1):59-63.
- [24]Tomaszewska-HetmanL, RywinskaA. Erythritol biosynthesis from glycerol by Yarrowia lipolytica yeast:effect of osmotic pressure[J].Chemical Paper,2016,70(3):2472-2483.
- [25]Heejung M,Jeya M,Inwon K,et al.Biotechnological production of erythritol and its applications[J].Appl Microbiol Biotechnol,2010,86(4):1017-1025.
- [26]Aleksandra M,Dobrowolski A,Rakicka M,et al.Newly isolated mutant of Yarrowia lipolytica MK1as a proper host for efficient erythritol biosynthesis from glycerol[J].Process Biochemistry,2015,50(1):61-68.
- [27]Katsuhiko S,ArihiroT,Takashi Y,et al.Key role for transketolaseactivityinerythritolproductionby Trichosporonoides megachiliensis SN-G42[J].Journal of Bioscience and Bioengineering,2009,108(5):385-390.
- [28]刘俊梅,王辉,李琢伟,等.赤藓糖醇合成途径及关键酶的研究进展[J].农产品工程,2015(5):58-61.
- [29]Dorota R,Cécile N,Hugo D,et al.EUF1-a newly identifed gene involved in erythritol utilization in Yarrowia lipolytica[J].Science Report,2017,7(1):125-127.
- [30]Carly F,Gamboa-Melendez H,Vandermies M,et al.Identification and characterization of EYK1,a key gene for erythritol catabolism in Yarrowia lipolytica[J].Appl Microbiol Biotechnol,2017,101(17):6587-6596.
- [31]Tomasz J,Adam D,Anna B,et al.Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis[J].Microbial Cell Factories,2017,16(1):118.
- [32]Aleksandra M,Dorota R,Anna B,et al.A novel strain of Yarrowia lipolytica as a platform for value-added product synthesis from glycerol[J].Biotechnology for Biofuels,2016,9(1):180.
- [33]Frédéric C,Marie V,Samuel T,et al.Enhancing erythritol productivity in Yarrowia lipolytica using metabolic engineering[J].Metabolic Engineering,2017,42:19-24.
- [34]Ludwika T,Anita R,Waldemar R.High selectivity of erythritolproductionfromglycerolbyYarrowia lipolytica[J].Science Direct,2014,64:309-320.
- [35]Anita R,Marta M,Edmund C,et al.Optimization of medium composition for erythritol production from glycerol by Yarrowia lipolytica using response surface methodology[J].Preparative Biochemistry&Biotechnology,2015,45(6):515-529.
- [36]Magdalena R,Aleksandra M,Ludwika T,et al.An effective method of continuous production of erythritol from glycerol by Yarrowia lipolytica MK1[J].Food Technol Biotechnol,2017,55(1):125-130.
- [37]Libo Y,Xiaobei Z,Zhiyong Z,et al.A novel osmotic pressure control fed-batch fermentation strategy for improvement of erythritol production by Yarrowia lipolyticafrom glycerol[J].Bioresource Technology,2014,151(1):120-127.
- [38]杨利博,郑志永,詹晓北.甘氨酸、脯氨酸促进高渗环境下Yarrowia lipolytica发酵甘油产赤藓糖醇[J].食品与发酵工业,2013,39(12):1-6.
- [39]Ludwika T,Waldemar R,Anita R.Mineral supplementation increases erythrose reductase activity in erythritol biosynthesis from glycerol by Yarrowia lipolytica[J].Appl Biochem Biotechnol,2014,172(6):3069-3078.
- [40]Magdalena R,Anita R,Krzyszt C,et al.Enhanced production of erythritol and mannitol by Yarrowia lipolytica in media containing surfactants[J].Brazilian Journal of Microbiology,2016,47(2):417-423.
- [41]Libo Y,Xiaomeng D,Zhiyong Z,et al.Proteomic analysis of erythritol-producing Yarrowia lipolytica from glycerol in response to osmotic pressure[J].J Microbiol Biotechnol,2015,25(7):1056-1069.