南极磷虾酶解液脱氟工艺研究Study on Defluorination Technology of Antarctic Krill Protein Hydrolysate
黄俊伟,崔春,郑雪君,陈智光
摘要(Abstract)：
为了探讨南极磷虾酶解液的高效脱氟工艺,首先比较了脱氟剂对含氟化钠溶液和南极磷虾酶解液的氟脱除效果差异,筛选出适合酶解液的最佳脱氟剂,以脱氟率和蛋白损失率为指标,采用正交试验优化酶解液脱氟工艺,最后对其氨基酸组成进行了分析。试验结果表明:聚合氯化铝对酶解液的脱氟效果最好,脱氟率为93.96%,而几丁质则完全没有脱氟能力。不同的无机钙盐对酶解液的氟脱除效果差异很大,其中氢氧化钙对酶解液的脱氟率高达71.28%,而磷酸一氢钙仅为2.17%;有机钙盐对酶解液的脱氟效果差异不大,脱氟率均超过50%。选用氢氧化钙作为脱氟剂,最优脱氟工艺条件为添加量0.5%,磷酸(1+5)调节反应pH为6.5,反应时间60min,温度40℃,此时的蛋白损失率和氨氮损失率分别为8.57%和8.26%,脱氟率高达99.27%,脱氟后酶解液氟含量仅为0.30mg/kg。氨基酸分析表明脱氟后酶解液的酪氨酸和胱氨酸损失严重,但是总氨基酸和必需氨基酸分别仅下降5.03%和2.71%。此工艺具有操作简便、反应时间短、成本低、营养价值损失少等优点,可为低氟南极磷虾产品的工业化生产提供理论依据。
关键词(KeyWords)： 南极磷虾;酶解液;脱氟;钙盐
基金项目(Foundation): 广州市科技计划项目(201604020067)
作者(Author): 黄俊伟,崔春,郑雪君,陈智光
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