贤乾隆,黄翠姬,杨振媚,刘昭明

• 1:广西工学院生物与化学工程系

摘要(Abstract)：

建立了高效液相色谱邻苯二甲醛(OPA)柱前衍生-紫外检测法对乳酸菌发酵液中γ-氨基丁酸的含量进行了测定。乳酸菌发酵液经离心,OPA柱前衍生后,利用Luna-C18色谱柱(250×4.6mm,5μm),以15mmol/mL醋酸钠缓冲液∶甲醇为55∶45为流动相,在流速为0.8mL/min,柱温30℃,紫外检测波长334nm的条件下进行测定。结果表明:γ-氨基丁酸在50～800μg/mL的浓度范围内线性关系良好,线性方程为y=5465.9x-5411(R2=0.9996),检测限为2.6714μg/mL,平均回收率为98.31%,RSD=2.27%。该方法操作简单、快速,分离度好,精密度高,满足痕量分析要求。

关键词(KeyWords)： HPLC;邻苯二甲醛;γ-氨基丁酸;乳酸菌

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 贤乾隆,黄翠姬,杨振媚,刘昭明

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