双水相法提取天然辣椒碱Aqueous Two-phase Extraction for Capsaicin
范勇,卢艳敏,檀琮萍,梁艳,崔波
摘要(Abstract):
采用双水相体系L44/Na_2CO_3提取辣椒碱,考察体系中L44含量、Na_2CO_3含量、乙醇含量、缓冲液pH、样品添加量、提取时间对辣椒碱提取率的影响。以L44含量、Na_2CO_3含量、缓冲液pH做正交试验,结果表明:双水相体系为L44/Na_2CO_3/乙醇为20%∶20%∶1%,缓冲液pH为2,双水相体系总重为10g,辣椒精为0.2g,提取时间为60min,辣椒碱的提取率达95.6%。
关键词(KeyWords): 双水相;提取;辣椒碱;正交试验;优化
基金项目(Foundation): 山东省高等学校科技计划项目(J11LC12)
作者(Author): 范勇,卢艳敏,檀琮萍,梁艳,崔波
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