舒雨竹,姚文博

• 1:陕西科技大学

摘要(Abstract)：

采用水提法提取枸杞中的活性成分，并选取料液比、酶解温度、酶解pH 3个单因素，在单因素实验结果的基础上利用响应面法对多糖和蛋白质的提取工艺进行优化，得到的最佳提取工艺为料液比1∶25.29、酶解温度59.29℃、酶解pH 5.94,多糖和蛋白质的理论含量为79.36%,在最佳提取工艺下提取3次，平均含量为79.05%,可见响应面法得到的提取工艺的稳定性与可靠性高。对提取物进行抗氧化活性测定，结果表明，枸杞提取物对超氧阴离子自由基有一定的清除作用，但清除作用不明显；对羟基自由基有较好的清除作用，在低浓度时与V_C相当；在DPPH自由基清除实验中，在较小质量浓度时其清除能力弱于V_C,但在较高质量浓度时其清除能力逐渐接近V_C,表明枸杞具有较强的抗氧化能力。

关键词(KeyWords)： 多糖;工艺优化;抗氧化

Abstract:

Keywords:

基金项目(Foundation): 陕西省科技厅科技计划-农业领域项目(2022NY-035);; 陕西省西安市科技计划项目(20NYYF0022,22NYYF048);; 陕西省西安市未央区科技计划项目(202040);; 陕西科技大学博士科研启动基金项目(2022BJ-29);; 陕西省教育厅2023年度一般专项科学研究计划项目(23JK0352)

作者(Author): 舒雨竹,姚文博

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