黎奇伟,刘晓兰,郑喜群,姜彩霞,褚佳盈

• 1:黑龙江八一农垦大学食品学院
• 2:齐齐哈尔大学食品与生物工程学院
• 3:黑龙江省玉米深加工理论与技术重点实验室
• 4:黑龙江八一农垦大学国家杂粮工程技术研究中心

摘要(Abstract)：

为提高解脂耶氏酵母(Yarrowia lipolytica)发酵生产赤藓糖醇的产量与过程可控性，该研究以高产菌株DX-UV-39为研究对象，综合采用多参数工艺优化与动力学模型构建相结合的策略，有效提升了赤藓糖醇产量并强化了过程控制能力。通过单因素试验和Box-Behnken响应面法系统优化发酵条件，确定最佳工艺为温度33.3℃、pH值6.3、发酵时间216.7 h、接种量10.3%。在该条件下，赤藓糖醇产量达到96.91 g/L。进一步建立的菌体生长、产物合成与底物消耗动力学模型拟合效果良好，结果显示，菌体生长呈典型S形曲线，赤藓糖醇合成属于部分生长耦联型，葡萄糖消耗与菌体生长和维持代谢密切相关。该研究通过工艺优化与动力学建模相结合，显著提高了赤藓糖醇产量，为发酵过程的精准调控与工业化放大提供了理论支持。

关键词(KeyWords)： 赤藓糖醇;解脂耶氏酵母;发酵工艺优化;动力学模型

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省重点研发计划项目(2022ZX02B15-2)

作者(Author): 黎奇伟,刘晓兰,郑喜群,姜彩霞,褚佳盈

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