温平平,夏超,于小番,许慧卿

• 1:扬州大学食品科学与工程学院

摘要(Abstract)：

杂环胺是肉制品加热过程中产生的一类具有强致癌、致突变能力的杂环化合物,近年来,随着人们生活水平的提高,杂环胺对人体健康的危害性越来越受到重视,文章简要介绍了杂环胺的分类、形成机理,并重点综述了常见杂环胺的检测方法及其抑制机理,目的是为杂环胺的相关研究提供系统性的理论指导。

关键词(KeyWords)： 肉制品;杂环胺;抑制;检测方法

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 温平平,夏超,于小番,许慧卿

参考文献(References)：

• [1]WIDMARK E M P.Presence of cancer-producing substances in roasted food[J].Nature,1939,143(3632):984.
• [2]SUGIMURA T,NAGO M,WEISBURGER J H.Muta-genic factors in cooked foods[J].CRC Critical Reviews in Toxicology,1979,6(3):189-209.
• [3]COMMANER B,VITHAYATHIL A J,DOLARA P,et al.Formation of mutagens in beef and beef extract during cooking[J].Science,1978,201(4359):913-916.
• [4]GROSS G A,GRUTER A.Quantitation of mutagengenic/carcinogenic heterocyclic aromatic amines in food products[J].Journal of Chromatography A,1992,592:271-278.
• [5]GIBIS M.Heterocyclic aromatic amines in cooked meat products:cause,formation,occurrence,and risk assessment[J].Compr Rev Food Sci and Food Saf,2016,15(2):269-302.
• [6]SKOG K,JAGERSTAD M.Incorporation of carbon atoms from glucose into the food mutagens MeIQx and 4,8-DiMeIQx using carbon-14-labeled glucose in a model system[J].Carcinogene,1993,14:2027-2031.
• [7]KIKUGAWA K.Involvement of free radicals in the formation of heterocyclic amines and prevention by antioxidants[J].Cancer Letters,1999,143(2):123-126.
• [8]KATO K,HARASHIMA T,MORIYA N,et al.Formation of the mutagenic/carcinogenic imidazoquinoxaline-type heterocyclic amines through the unstable free radical maillard imtermediates and its inhibition by phenolic antioxidants[J].Carcinogenesis,1996,17(11):2469-2476.
• [9]PEARSON A M,CHEN C H,GRAY J I,et al.Mechanism(s) involved in meat mutagen formation and inhibition[J].Free Radical Biology and Medicine,1992,13(2):161-167.
• [10]ZAMORA R,ALCON E,HIDALGO F J.Ammonia and formaldehyde participate in the formation of 2-amino-1-methyl-6phenylimidazo[4,5-b]pyridine(PhIP) in addition to creat(ni) ne and phenylacetaldehyde[J].Food Chemistry,2014,155:74-80.
• [11]ARKADIUSZ S.Chemical state of heterocyclic aromatic amines in grilled beef:evaluation by in vitro digestion model andcomparison of alkaline hydrolysis and organic solvent for extraction[J].Food and Chemical Toxicology,2013,62:653-660.
• [12]CHARLES F M,NATALIA P V,THU H P,et al.Rapid determination of heterocyclic amines in ruminant meats using accelerated solvent extraction and ultra-high performance liquid chromatograph-mass spectrometry[J].Methods X,2019,6:2686-2697.
• [13]HOLLAND R D,TAYLOR J,SCHOENBACHLER L,et al.Rapid biomonitoring of heterocyclic aromatic amines in human urine by tandem solvent solid phase extraction liquid chromatography electrospray ionization mass spectrometry[J].Chemical Research in Toxicology,2004,17:1121.
• [14]ZHANG Yuan,WU Wenjie,ZHOU Weie,et al.Determination of 14 heterocyclic aromatic amines in meat products using solid-phase extraction and supercritical fluid chromatography coupled to triple quadrupole mass spectrometry[J].Journal of Separation Science,2020,43(7):1372-1381.
• [15]王强强，廖惠云，姚忠，等.固相微萃取和溶剂萃取结合GC-MS分析八角茴香化学成分[J].中国调味品，2020,45(10):130-134.
• [16]ZHANG Qianchun,CHENG Yingyi,LI Gongke,et al.Porous boronate affinity monolith for on-line extraction coupled to high-performance liquid chromatography for sensitive analysis of heterocyclic aromatic amines in food samples[J].Chinese Chemical Letters,2015,26(12):1470-1477.
• [17]FERNANDO D A,MOHAMMED Z,GREGORIO C,et al.Simultaneous determination of six non-polar heterocyclic amines in meat samples by supercritical fluid extraction-capillary electrophoresis under fluorimetric detection[J].Electrophoresis,2010,31(13):2165-2173.
• [18]周亚.气质联用技术在桂皮挥发性成分检测分析过程中的应用[J].中国调味品，2020,45(10):138-140,143.
• [19]WARZECHA L,JANOSZKA B,BLASZCZYK U,et al.Determination of heterocyclic aromatic amines(HAs)content in samples of household-prepared meat dishes[J] Journal of Chromatography B,2004,802(1):95-106.
• [20]BARCELO B E,MOYANO E,GALCERN N T,et al.Uitra-perforrmance liquid chromatography-tandem mass spectrometry for the anaiysis of heterocyclic amines in food[J].Journal of Chromatography A,2006,1125(2):195-203.
• [21]徐琦，杨洪生，吴光红，等.超高效液相色谱-电喷雾串联质谱法同时测定鱼类加工品中12种杂环胺类化合物[J].南方水产科学，2012,8(4):7-15.
• [22]侯瑾，李迎秋.酶联免疫吸附技术在食品安全检测中的应用[J].中国调味品，2017,42(6):165-169.
• [23]MARTIN V,BRUCE E W,MONA H,et al.Monoclonal antibodies to 2-amino-l-methyl-6-phenylimidazo[4,5-b\]pyridine(PhIP)and their use in the analysis of well-done fried beef[J].Carcinogenesis,1989,10(12):2215-2221.
• [24]SHENG Wei,RAN Xuqin,HU Gaoshuang,et al.Development of an enzyme-linked immunosorbent assay for the detection of 2-amino-3-methylimidazo[4,5-f]quinoline(IQ)in processed foods[J].Food Analytical Methods,2016,9(4):1036-1045.
• [25]DE ANDRéS F,ZOUGAGH M,CASTANEDA G,et al.Screening of non-polar heterocyclic amines in urine by microextraction in packed sorbent-fluorimetric detection and confirmation by capillary liquid chromatography[J].Talanta,2011,83(5):1562-1567.
• [26]FENG Ruihong,BAO Yulong,LIU Dongmei,et al.Steam-assisted roasting inhibits formation of heterocyclic aromatic amines and alters volatile flavour profile of beef steak[J].International Journal of Food Science & Technology,2020,55(9):3061-3072.
• [27]曾茂茂，陈静，陈洁，等.一种基于胺类活性成分降低结合态杂环胺含量的方法：中国，CN107183507A[P].2017-09-22
• [28]CHENG Yiqun,YU Yajie,WANG Chong,et al.Inhibitory effect of sugarcane (Saccharum officinarum L.) molasses extract on the formation of heterocyclic amines in deep-fried chicken wings[J].Food Control,2021,119:107490.
• [29]KESKEKOGLU H,UREN A.Inhibitory effects of grape seed extract on the formation of heterocyclic aromatic amines in beef and chicken meatballs cooked by different techniques[J].International Journal of Food Properties,2017,20(Suppl 1):722-734.
• [30]李少觐.肉类风味影响因素研究进展[J].中国调味品，2020,45(2):188-191.
• [31]王德振，李佳，张玲玲，等.大蒜、洋葱、生姜和辣椒四种香辛料风味成分研究进展[J].中国调味品，2019,44(1):179-185.
• [32]TSAI S J,JENQ S N,LEE H.Naturally occurring diallyl disulfide inhibits the formation of carcinogenic heterocyclic aromatic amines in boiled pork juice[J].Mutagenesis,1996,11(3):235-240.
• [33]李利洁.微波加热中杂环胺PhIP的形成规律及抑制机制[D].无锡：江南大学，2018.
• [34]FELTON J S,FULTZ E,DOLBEARE F A,et al.Effect of microwave pretreatment on heterocyclic aromatic amine mutagens/carcinogens in fried beef patties[J].Food and Chemical Texicology,1994,32(10):897-903.
• [35]HASNOL N D S,JINAP S,SANNY M.Effect of different types of sugars in a marinating formulation on the formation of heterocyclic amines in grilled chicken[J].Food Chemistry,2014,145:514-521.
• [36]PERSSON E,SJOHOIM I,NYMAN M,et al.Addition of various carbohydrates to beef burgers affects the formation of heterocyclic amines during frying[J].Journal of Agricultural and Food Chemistry,2004,52(25):7561-7566.
• [37]MEURILLON M,ENGEL E.Mitigation strategies to reduce the impact of heterocyclic aromatic amines in proteinaceous foods[J].Trends in Food Science & Technology,2016,50:70-84.
• [38]REIS S A D,CONCEIC?O L L D,SIQUEIRA N P,et al.Review of the mechanisms of probiotic actions in the prevention of colorectal cancer[J].Nutrition Research,2017,37:1-19.
• [39]HUBER W W,PRUSTOMERSKY S,DELBANCO E,et al.Enhancement of the chemoprotective enzymes glucuronosyl transferase and glutathione transferase in specific organs of the rat by the coffee components kahweol and cafestol[J].Archives of Toxicology Supplement,2002,76(4):209-217.
• [40]HO V,BRUNETTI V,Peacock S,et al.Exposure to meat-derived carcinogens and bulky DNA adduct levels in normal-appearing colon mucosa[J].Mutation Research/Genetic Toxicology and Environmental Mutagenesis,2017,821:5-12.