不同醋酸菌发酵对紫甘薯醋理化特性、抗氧化活性及感官特性的影响Effects of Fermentation of Different Acetic Acid Bacteria on Physicochemical Properties, Antioxidant Activity and Sensory Properties of Purple Sweet Potato Vinegar
丁羽洁,孙红男,马梦梅,木泰华
摘要(Abstract):
该研究以徐紫薯8号为原料,采用红曲霉和酵母菌协同酒精发酵后,使用5株不同醋酸菌发酵制备紫甘薯醋,测定不同醋酸菌发酵紫甘薯醋的理化指标、营养与功能成分、感官特性和抗氧化活性。在此基础上,采用灰色理论关联度分析筛选出适宜紫甘薯醋发酵的醋酸菌菌株。结果表明,不同紫甘薯醋的总酸含量和可溶性无盐固形物含量均符合国家标准的规定(总酸含量≥3.5 g/100 mL,可溶性无盐固形物含量≥0.5 g/100 mL),其中,以醋酸菌CICC20011发酵制备的紫甘薯醋(SPM5)的总酸含量(4.89 g/100 mL)、可溶性无盐固形物含量(2.41 g/100 mL)、氨基酸态氮含量(1.19 g/100 mL)、γ-氨基丁酸含量(1.10μg/mL)、总酚含量(151.63 mg GAE/100 mL)及DPPH自由基清除率(72.46 mg TE/mL)均达到最高。通过灰色理论关联度分析,醋酸菌CICC20011发酵制备的紫甘薯醋(SPM5)的综合评分最高,表明该醋酸菌适宜作为紫甘薯醋的发酵菌株。该研究结果为紫甘薯醋的工业化生产提供了理论指导。
关键词(KeyWords): 醋酸菌;紫甘薯醋;理化特性;抗氧化活性;感官特性;灰色理论关联度
基金项目(Foundation): 国家甘薯产业技术体系资助(CARS-10)
作者(Author): 丁羽洁,孙红男,马梦梅,木泰华
参考文献(References):
- [1]SUBAWA I W,ASTAWA P,BAKTA I M,et al.Purple sweet potato(Ipomoea batatas L.) extract effects on levels of inflammatory markers and chondrocyte count in gout arthritis Wistar rat model[J].Foot and Ankle Surgery,2023,29(8):611-615.
- [2]DING X,FAN H.Purple sweet potato polysaccharide ameliorates concanavalin A-induced hepatic injury by inhibiting inflammation and oxidative stress[J].Phytomedicine,2024,129:155652.
- [3]马天龙,孙振兴,郭汝悦.紫薯花青素组成及对运动疲劳小鼠糖代谢和肝脏氧化应激的影响[J].中国食品添加剂,2024,35(7):80-88.
- [4]LI R,XIAO R S,HE S J,et al.Research advances of purple sweet potato anthocyanins:extraction,identification,stability,bioactivity,application,and biotransformation[J].Molecules,2019,24(21):3816.
- [5]RODA A,LUCINI L,TORCHIO F,et al.Metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste[J].Food Chemistry,2017,229:734-742.
- [6]NEFFE-SKOCI??SKA K,KARBOWIAK M,KRUK M,et al.Polyphenol and antioxidant properties of food obtained by the activity of acetic acid bacteria(AAB)-a systematic review[J].Journal of Functional Foods,2023,107:105691.
- [7]WU F,YAO H L,CAO X M,et al.Production of vinegar from purple sweet potato in liquid fermentation process and investigation of its antioxidant activity[J].3 Biotech,2017,7(5):308.
- [8]张村雪,朱渭兵,李志西,等.紫甘薯醋及其不同发酵阶段产物的抗氧化活性[J].食品科学,2013,34(11):88-93.
- [9]曹桢,陈善敏,黄小雨,等.紫薯醋发酵工艺优化及品质分析[J].食品与发酵工业,2021,47(4):195-201.
- [10]MATURANO P,MESTRE M V,KUCHEN B,et al.Optimization of fermentation-relevant factors:strategy to reduce ethanol in red wine by sequential culture of native yeasts[J].International Journal of Food Microbiology,2019,289(3):40-48.
- [11]何珺珺,时伟,李喆,等.响应面法优化红曲霉发酵酒糟产洛伐他汀工艺[J].中国酿造,2020,39(8):77-81.
- [12]郭鑫磊,王宏霞,施明丽,等.不同陈酿年份四大名醋有机酸及挥发性风味物质比较分析[J].中国酿造,2023,42(7):58-64.
- [13]纪凤娣,魏巍,陶汇源,等.食醋中总多酚的测定方法及其不同陈醋产品的含量比较[J].中国调味品,2021,46(10):100-104.
- [14]KONG H,KIM H,JEONG W S,et al.Microbiome analysis of traditional grain vinegar produced under different fermentation conditions in various regions in Korea[J].Foods,2022,11(22):3573.
- [15]ZHU H,ZHU J,WANG L,et al.Development of a SPME-GC-MS method for the determination of volatile compounds in Shanxi aged vinegar and its analytical characterization by aroma wheel[J].Journal of Food Science and Technology,2016,53(1):171-183.
- [16]SUI Y,ZHU Z Q,CHENG F S,et al.Dynamic changes and correlation of quality,flavor and microorganisms of Mei(Prunus mume) vinegar during fermentation and clarification[J].Food Research International,2024,197(3):115209.
- [17]YIN N,JIANG J Q,MA X M,et al.Dynamic changes of physicochemical parameters,antioxidant activity,organic acids,polyphenols,and volatile components in prune vinegar during fermentation[J].Food Bioscience,2024,59(23):104042.
- [18]LI L,HUANG X,LI Z C,et al.Investigating the temporal evolution of physicochemical attributes and flavorome profiles in Sichuan shai vinegar utilizing diverse aging techniques[J].Food Research International,2024,196:115080.
- [19]陈景智,林娟,叶秀云,等.紫色红曲霉液态发酵产糖化酶的工艺研究[J].食品工业科技,2017,38(14):146-150,155.
- [20]祁勇刚,董红兵,蔡凤娇,等.不同醋酸菌对菠萝糯米果粮醋风味的影响[J].中国调味品,2024,49(9):9-14.
- [21]LI J,ZHANG Y,FAN J F.Acetic acid in aged vinegar affects molecular targets for thrombus disease management[J].Food Function,2015,6(8):2845-2853.
- [22]BAI H,WANG S,WANG M,et al.Investigation of bioactive compounds and their correlation with the antioxidant capacity in different functional vinegars[J].Food Research International,2024,184:114262.
- [23]文镜,张静,常平,等.功能红曲醋的制备及其降血脂功能的研究[J].食品科学,2007,28(9):510-513.
- [24]杜琳琳,赵祥颖,刘丽萍,等.红曲霉菌的筛选及其在液态食醋酿造中的应用[J].中国调味品,2024,49(1):33-39.
- [25]RUAN W,LIU L,LI P L,et al.Flavor production driven by microbial community dynamics and their interactions during two-stage fermentation of Ziziphus jujuba vinegar[J].Food Bioscience,2024,57(4):103537.
- [26]WANG H,WANG M Y,CAO L W,et al.Changes and correlation of microorganism and flavor substances during persimmon vinegar fermentation[J].Food Bioscience,2022,46(11):101565.
- [27]刘璐,韩艳文,王楠,等.紫薯醋对小鼠急性肝损伤保护及减肥降脂作用[J].西北农业学报,2015,24(1):28-33.
- [28]TONG G,LI W L,RAO Y D,et al.Microbiomics and metabolomics insights into the microbial regulation on the formation of flavor components in the traditional fermentation process of Chinese Hongqu aged vinegar[J].Food Science and Human Wellness,2024,13(5):2765-2778.
- [29]YANG H R,CHEN T,WANG M,et al.Molecular biology:fantastic toolkits to improve knowledge and application of acetic acid bacteria[J].Biotechnology Advances,2022,58(6):107911.
- [30]郭蕾,孙浩,宋来生,等.基于风味检测技术对四大名醋挥发性成分的研究进展[J].中国酿造,2022,41(6):12-17.
- [31]武雪,任静宇,吕伟,等.4种桂花香醋挥发性风味和有机酸成分分析[J].中国调味品,2023,48(7):162-168,174.
- [32]高航,续丹丹,王文平,等.基于多元统计学分析红曲米醋醋酸发酵阶段挥发性风味特征组分[J].食品科学,2022,43(12):219-227.
- [33]AL-DALALI S,ZHENG P,LI H H,et al.Characterization of volatile compounds in three commercial Chinese vinegars by SPME-GC-MS and GC-O[J].LWT-Food Science and Technology,2019,112(2):108264.
- [34]ZHOU L,JIAN D Z,GONG M,et al.Characterization of the key aroma compounds in aged Zhenjiang aromatic vinegar by gas chromatography-olfactometry-mass spectrometry,quantitative measurements,aroma recombination and omission experiments[J].Food Research International,2020,136(8):109434.
- [35]AL-DALALI S,ZHENG P,SUN B G,et al.Effects of different brewing processes on the volatile flavor profiles of Chinese vinegar determined by HS-SPME-AEDA with GC-MSand GC-O[J].Food Science and Technology,2020,133:109969.
- [36]杜大钊,黄静,王瑞,等.四川麸醋及其陈酿过程中挥发性风味物质解析[J].食品与发酵科技,2020,56(5):1-6.
- [37]张林祥,张蕾,秦子涵,等.顶空-固相微萃取-气质联用法分析玫瑰醋挥发性风味物质及特征[J].食品与发酵工业,2022,48(21):269-275.
- [38]GONG M,ZHOU L,LIU S P,et al.Dynamic changes in physico-chemical attributes and volatile compounds during fermentation of Zhenjiang vinegars made with glutinous and non-glutinous japonica rice[J].Journal of Cereal Science,2021,100:103246.
- [39]奚佳玉,苏圆锦,董树清,等.微生物发酵转化黄酮类化合物研究进展[J].食品科学,2024,45(17):326-334.
- [40]??ZDEMIR N,PASHAZADEH H,ZANNOU O,et al.Phytochemical content,and antioxidant activity,and volatile compounds associated with the aromatic property,of the vinegar produced from rosehip fruit(Rosa canina L.)[J].LWT-Food Science and Technology,2022,154(7):112716.
- [41]XIA T,QIANG X,GENG B,et al.Changes in the phytochemical and bioactive compounds and the antioxidant properties of wolfberry during vinegar fermentation processes[J].International Journal of Molecular Sciences,2022,23(24):15839.
- [42]HONG Y,MU T H,SUN H N,et al.Valorization of the green waste parts from sweet potato(Impoea batatas L.):nutritional,phytochemical composition,and bioactivity evaluation[J].Food Science and Nutrition,2020,8(8):4086-4097.