优化提取潼川豆豉中抗氧化成分及其抗氧化活性研究Study on Optimization of Extraction of Antioxidant Components from Tongchuan Fermented Soya Beans and the Antioxidant Activity
池玉闽,贾利蓉,邓莎,王书语,向燕,何培君,董怡
摘要(Abstract):
利用超声波辅助提取潼川豆豉中抗氧化成分,通过响应面法对提取工艺进行优化,最终确定乙醇浓度37%、液料比26∶1 (mL/g)、超声时间10.5 min、超声功率509 W为最佳提取条件,在此条件下,可以得到总酚含量68.70 mg/g、异黄酮含量5.11 mg/g、DPPH·清除能力13.44 mg Trolox/g。潼川豆豉提取物对DPPH·和ABTS~+的清除能力的EC_(50)值分别为0.58,3.10 mg/L,ORAC值为6.56 g Trolox/g,还原力为0.51 g Trolox/g,均明显优于没食子酸。使用LC-MS从潼川豆豉提取液中分析出游离氨基酸、大豆异黄酮、大豆皂苷等50种物质,含量较高的小分子蛋白质降解物、大豆异黄酮可能是潼川豆豉抗氧化能力的主要物质基础。
关键词(KeyWords): 潼川豆豉;抗氧化;响应面法;超声辅助提取
基金项目(Foundation): 高校基本科研项目(20826041D4060);; 四川省国际科技创新合作项目(2021YFH0043)
作者(Author): 池玉闽,贾利蓉,邓莎,王书语,向燕,何培君,董怡
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