程立坤,胡悦,王思明,李姣灿,姚慧静,王国泽,张亚琨

• 1:内蒙古科技大学生命科学与技术学院
• 2:包头市农牧业科学技术研究所

摘要(Abstract)：

目的：优化微波辅助提取辣椒红素工艺，研究不同品种辣椒的辣椒红素的抗氧化活性。方法：以包头市农科院提供的不同品种的红辣椒为实验材料，采用微波辅助法，通过单因素实验和响应面实验，以辣椒红素含量为指标，进行四因素三水平分析，分别为液料比(4∶1、6∶1、8∶1)、提取时间(25,30,35 min)、微波功率(550,600,650 W)、提取温度(50,55,60℃)。提取出辣椒红素后从5个方面进行体外抗氧化活性评价，即铁离子还原能力测定、DPPH自由基清除能力测定、ABTS自由基清除能力测定、羟自由基(·OH)清除能力测定、超氧阴离子自由基(O_2~-·)清除能力测定，并对不同品种辣椒的抗氧化活性进行比较。结果：优化微波辅助提取辣椒红素的最佳工艺为液料比6∶1、微波功率600 W、提取时间30 min、提取温度60℃,在此条件下提取的辣椒红素平均含量为47.809 mg/g,得率为4.7%。对提取的辣椒红素进行体外抗氧化活性评价，结果表明不同品种的红辣椒均具有抗氧化活性，且因其含有的辣椒红素浓度不同，对DPPH自由基、ABTS自由基、·OH和O_2~-·的清除率和抑制率不同，综合来看，金畯1号的抗氧化能力最强，对DPPH自由基、ABTS自由基、羟自由基和超氧阴离子自由基清除率的IC_(50)值分别为0.909,0.467,15.778,2.504 mg/mL。该研究为辣椒品种选育、深加工和辣椒红素深入研究提供了参考依据。

关键词(KeyWords)： 辣椒;辣椒红素;微波辅助提取;响应面实验;抗氧化活性评价

Abstract:

Keywords:

基金项目(Foundation): 内蒙古自治区科技厅攻关项目(2020GG0097);; 内蒙古自治区高等学校碳达峰碳中和研究专项(STZX202229)

作者(Author): 程立坤,胡悦,王思明,李姣灿,姚慧静,王国泽,张亚琨

参考文献(References)：

• [1]陶兴林，朱惠霞，王晓巍，等.5个不同产区地理标志辣椒营养成分分析[J].食品安全质量检测学报，2023,14(3):303-310.
• [2]LIU Z B,MAO L Z,HUANG Y,et al.Changes of fruit quality in five different genotypes of pepper (Capsicum annuum L.) during development[J].Food Science,2021,42(23):18-26.
• [3]KOOCHAK H,PUTHIYAVEETIL S,MULLENDORE D L,et al.The structural and functional domains of plant thylakoid membranes[J].The Plant Journal,2019,97(3):412-429.
• [4]MOREB N,O'DWYER C,JAISWAL S,et al.Pepper[M]//Nutritional Composition and Antioxidant Properties of Fruits and Vegetables,New York:Academic Press,2020:223-238.
• [5]MAEDA H,NISHINO A,MAOKA T.Biological Activities of Paprika Carotenoids,Capsanthin and Capsorubin[M]//Carotenoids:Biosynthetic and Biofunctional Approaches,Cham:Springer,2021:285-293.
• [6]PALMA J M,TERáN F,CONTRERAS-RUIZ A,et al.Antioxidant profile of pepper (Capsicum annuum L.) fruits containing diverse levels of capsaicinoids[J].Antioxidants,2020,9(9):878-896.
• [7]HIROSHI M,HIROMICHI N,MAKOTO C,et al.Antioxidant activity of capsanthin and the fatty acid esters in paprika (Capsicum annuum)[J].Journal of Agricultural & Food Chemistry,1998,46(9):3468-3472.
• [8]EKWERE M,UDO D E.Potential health benefits of conventional nutrients and phytochemicals of Capsicum peppers[J].Pharmacy & Pharmacology International Journal,2018,6(1):62-69.
• [9]MAKOTO T,HIDEO E,KYUKI K,et al.Nitrocapsanthin and nitrofucoxanthin,respective products of capsanthin and fucoxanthin reaction with peroxynitrite[J].Journal of Agricultural and Food Chemistry,2011,59(19):10572-10578.
• [10]KENNEDY L E,ABRAHAM A,KULKARNI G,et al.Capsanthin,a plant-derived xanthophyll:a review of pharmacology and delivery strategies[J].AAPS PharmSciTech,2021,22(5):203.
• [11]SINGH B,KALIA P.Vegetables for Nutrition and Entrepreneurship[M].Cham:Springer,2023.
• [12]LI Z H,WANG T.Progress in understanding the bioavailability,physiological functions and mechanisms of action of capsanthin[J].Food Science,2020,41(11):259-266.
• [13]岳盈肖，齐立军，黄利勇，等.辣椒红色素稳定性与应用研究进展[J].中国食品添加剂，2022,33(10):279-283.
• [14]张超，马静静，刘青，等.天然食用色素——辣椒红色素的制备及其应用方式[J].中国食品添加剂，2022,33(7):225-231.
• [15]BARBERO G F,PALMA M,BARROSO C G.Determination of capsaicinoids in peppers by microwave-assisted extraction-high-performance liquid chromatography with fluorescence detection[J].Analytica Chimica Acta,2006,578(2):227-233.
• [16]ANCY A,KANIMOZHI N V,ASHOK K S,et al.A review of extraction and quantification of capsaicin and its bio insecticidal activity in food grains[J].Preparative Biochemistry & Biotechnology,2024,54(3):435-443.
• [17]DE AGUIAR A C,VIGANó J,DA SILVA ANTHERO A G,et al.Supercritical fluids and fluid mixtures to obtain high-value compounds from Capsicum peppers[J].Food Chemistry:X,2022,13(10):100228.
• [18]MANDAL V,MOHAN Y,HEMALATHA S.Microwave assisted extraction—an innovative and promising extraction tool for medicinal plant research[J].Pharmacognosy Reviews,2007,1(1):7-18.
• [19]游国叶，史琼，杜晶.星点设计-响应面法优化密闭微波辅助提取辣椒素工艺[J].中国现代应用药学，2021,38(8):959-965.
• [20]KIM J S,PARK J T,AHN J,et al.Optimization of accelerated solvent extraction of capsanthin from red paprika(Capsicum annuum L.) using response surface methodology[J].Food Science and Technology Research,2019,25(4):519-528.
• [21]GOGUS F,OZEL M Z,KESKIN H,et al.Volatiles of fresh and commercial sweet red pepper pastes:processing methods and microwave assisted extraction[J].International Journal of Food Properties,2015,18(8):1625-1634.
• [22]程立坤，胡悦，张亚琨，等.不同品种辣椒营养品质、辣椒红素含量的差异比较及聚类分析[J].中国调味品，2024,49(1):133-140.
• [23]湖南省质量技术监督局.辣椒红色素含量测定取样规程：DB43/T 993—2015[S].2015.
• [24]张海红，田洪芸，毕婷婷，等.分光光度法检测食品添加剂茶黄素含量[J].中国食品卫生杂志，2022,34(4):761-766.
• [25]杜苏萌，马文亮，贾子昌，等.辣椒红色素提取工艺优化与分析研究[J].食品工程，2019(1):24-27,64.
• [26]YANG J Y,SHANG L C,ZHANG C.Extraction of polysaccharides and proteins from selenium-enriched Ganoderma lucidum mycelium and their in vitro antioxidant and antitumor activities[J].Natural Product Research and Development,2023,35(3):453-459.
• [27]李杰，田思雨，谭怡然，等.山楂叶多糖提取工艺优化及体外抗氧化和抑菌活性研究[J].食品工业，2023,44(4):91-95.
• [28]CHEN Y P,ZHAGN Y L,WU Y L,et al.Optimization of extraction technology and antioxidant activity of total flavonoids from roots of Cichorium Intybus L.by ultrasonic assisted with complex enzyme[J].Science and Technology of Food Industry,2021,42(8):164-171.
• [29]LIU H M,HUANG J G,YANG S F,et al.Chemical composition,algicidal,antimicrobial,and antioxidant activities of the essential oils of Taiwania flousiana Gaussen[J].Molecules,2020,25(4):967.
• [30]何莉媛，吴伟，吴晓娟，等.米糠贮藏时间对米糠蛋白体外胃蛋白酶消化性质及其消化产物抗氧化性的影响[J].中国油脂，2017,42(11):70-74.
• [31]李甜甜，林意雯，王飞，等.提取工艺对辣椒红素色价及得率的影响[J].食品研究与开发，2021,42(23):86-93.
• [32]池春欢，汪云友，陈厚荣.多指标综合评分法优化辣椒热泵-微波联合干燥工艺[J].食品与发酵工业，2018,44(6):172-179.
• [33]杜若君，石召华，詹志来，等.响应面法优化金莲花总酚酸的提取工艺及其美白活性研究[J].天然产物研究与开发，2023,35(6):915-924.
• [34]霍瑞春，王国泽，姚慧静，等.19种加工辣椒辣椒素提取、纯化及其含量测定[J].中国调味品，2022,47(4):167-172.
• [35]杨诗涵，李紫菡，张金山，等.酶协同微波提取云南沙棘果实总黄酮及其抗氧化活性评价[J].中国食品添加剂，2023,34(3):19-26.
• [36]PANG M,LIU Q,LI Y Y,et al.Ultrasonic-microwave synergistic extraction of paprika pigment[C]//E3S Web of Conferences,EDP Sciences,2019.
• [37]高经梁，刘玉兰，张惠娟，等.不同提取方法对辣椒红色素提取得率的比较研究[J].中国调味品，2012,37(10):105-107,112.
• [38]ABBASIAN F,ALAVI M S,ROOHBAKHSH A.Dietary carotenoids to improve hypertension[J].Heliyon,2023,9(9):19399.
• [39]MICHALAK M.Plant-derived antioxidants:significance in skin health and the ageing process[J].International Journal of Molecular Sciences,2022,23(2):585-613.
• [40]HASSAN M N,YUSOF N A,YAHAYA A F,et al.Carotenoids of capsicum fruits:pigment profile and health-promoting functional attributes[J].Antioxidants,2019,8(10):469-493.