调味香料草果中多酚类化合物抗氧化活性成分的快速筛选Rapid Screening of Antioxidant Active Components of Polyphenols in Spice Amomum tsaoko
杜丽霞,姜子涛,周烜
摘要(Abstract):
采用微波辅助提取法从调味香料草果中提取了多酚类化合物。用高效液相色谱-串联质谱法(LC-MS/MS)对草果多酚提取物化学成分进行了分析。以1,1-二苯基-2-三硝基苯肼自由基(DPPH)为探针,在未涉及复杂费时的单体分离的前提下,对多酚提取物中每个多酚类化合物的抗氧化活性进行了评价。结果表明,草果多酚类化合物的最佳提取条件是乙醇浓度70%、提取温度70℃、料液比1∶35(g/mL)、提取时间10 min,草果多酚类化合物的得率为11.13%;鉴定出3种多酚类化合物为原儿茶醛、汉诺基诺醇和双氢杨梅树皮素二聚体;在草果多酚类化合物中起主要抗氧化作用的是主成分汉诺基诺醇,其他多酚类化合物含量较低,活性存在较大差异,起辅助作用。
关键词(KeyWords): 草果;多酚;汉诺基诺醇;抗氧化;LC-MS/MS
基金项目(Foundation): 天津市高等学校自然科学研究项目(2021KJ095);; 天津市科技计划项目(20YDTPJC01610)
作者(Author): 杜丽霞,姜子涛,周烜
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