丁小强,陈丽丽,白春清,袁美兰,赵利

• 1:江西科技师范大学生命科学学院国家淡水鱼加工技术研发分中心

摘要(Abstract)：

以草鱼鱼糜漂洗液回收蛋白为原料,短肽得率和水解度为指标,从5种常用蛋白酶中选出碱性蛋白酶作为酶解回收蛋白的最适酶,通过单因素和响应面优化试验得出最佳酶解条件:酶解温度为52.51℃,酶解pH为9.06,酶添加量为1.42%,酶解时间为60min,酶解底物蛋白浓度为1g/dL。在此条件下回收蛋白的水解度为20.86%,短肽得率为73.5%;比较酶解前后回收蛋白的功能性质,发现酶解后多肽的溶解性、乳化性和起泡能力有所增加,乳化稳定性、泡沫稳定性和持油性较酶解前有所降低。

关键词(KeyWords)： 碱性蛋白酶;蛋白质;水解度;多肽;功能性质

Abstract:

Keywords:

基金项目(Foundation): 江西省高等学校科技落地计划项目(KJLD12009);; 江西省现代农业产业技术体系建设专项资金资助(赣财教指2013-258);; 国家农业科技成果转化项目(国科办农[2014]42号)

作者(Author): 丁小强,陈丽丽,白春清,袁美兰,赵利

参考文献(References)：

• [1]汪之和,陶妍,刘振华.白鲢漂洗鱼糜和未漂洗碎鱼肉营养成分的分析比较[J].淡水渔业,1999,29(8):16-18.
• [2]丁小强,赵利,付雪军,等.鱼糜漂洗液蛋白质回收工艺的研究[J].科学养鱼,2016(2):76-78.
• [3]Huang L,Morrissey M T.Fouling of membranes during microfiltration of surimi wash water:roles of pore blocking and surface cake formation[J].Journal of Membrane Science,1998,144(1):113-123.
• [4]Honer C.Nature's"nearly-perfect protein"[J].Dairy Food,1986,87:45.
• [5]Nishioka F,Shimizu Y.Recovery of proteins from washings of minced fish meat by pH-shifting method[J].Bulletin of the Japanese Society of Scientific Fisheries(Japan),1983,49:795-800.
• [6]Chavan U D,McKenzie D B,Shahidi F.Functional properties of protein isolates from beach pea(Lathyrus maritimus L.)[J].Food Chemistry,2001,74(2):177-187.
• [7]袁斌,吕桂善,刘小玲.蛋白质水解度的简易测定方法[J].广西农业生物科学,2002,21(2):113-115.
• [8]李雪,罗永康,尤娟.草鱼鱼肉蛋白水解物抗氧化性及功能特性研究[J].中国农业大学学报,2011,16(1):94-99.
• [9]Linder M,Fanni J,Parmentier M,et al.Protein recovery from veal bones by enzymatic hydrolysis[J].Journal of Food Science,1995,60(5):949-952.
• [10]Box G E P,Behnken D W.Some new three level designs for the study of quantitative variables[J].Technometrics,1960,2(4):455-475.
• [11]石岭.草鱼多肽粉及其功能性质的研究[D].南昌:江西科技师范大学,2014.
• [12]Ponnampalam R,Goulet G,Amiot J,et al.Some functional and nutritional properties of oat flours as affected by proteolysis[J].Journal of Agricultural and Food Chemistry,1987,35(2):279-285.
• [13]Pearce K N,Kinsella J E.Emulsifying properties of proteins:evaluation of a turbidimetric technique[J].Journal of Agricultural and Food Chemistry,1978,26(3):716-723.
• [14]Pedroche J,Yust M M,Lqari H,et al.Brassica carinata protein isolates:chemical composition,protein characterization and improvement of functional properties by protein hydrolysis[J].Food Chemistry,2004,88(3):337-346.
• [15]Bencina M.Functional properties of drum-dried chickpea flours[J].Journal of Food Science,1996,51:1518-1526.
• [16]张宇昊,王强酶.水解花生蛋白制备花生短肤的研究[J].农业工程学报,2007,23(4):258-263.
• [17]Tello P G,Camacho F,Jurado E,et al.Enzymatic hydrolysis of whey protein:I kinetic models[J].Biotechnology and Bioengineering,1994,44(4):523-528.
• [18]Rastogi N K,Rashmi K R.Optimization of enzymatic liquefaction of mango pulp by response surface methodology[J].European Food Research and Technology,1999,209(1):57-62.
• [19]朱艳华.玉米多肽的制备、理化性质及生物活性的研究[D].武汉:华中农业大学,2007.
• [20]Nie L,Siebert K J.Modeling physicochemical properties and activity of aspartyl proteinases based on amino acid composition[J].Journal of Agricultural and Food Chemistry,2009,57(6):2536-2543.
• [21]孙英.茶籽饼粕多肽的制备、纯化及抗氧化活性研究[D].广州:华南农业大学,2013.
• [22]Qi M,Hettiarachchy N S,Kalapathy U.Solubility and emulsifying properties of soy protein isolates modified by pancreatin[J].Journal of Food Science,1997,62(6):1110-1115.
• [23]Lawal O S,Adebowale K O,Ogunsanwo B M,et al.On the functional properties of globulin and albumin protein fractions and flours of African locust bean(Parkia biglobossa)[J].Food Chemistry,2005,92(4):681-691.
• [24]Shahidi F,Han X Q,Synowiecki J.Production and characteristics of protein hydrolysates from capelin(Mallotus villosus)[J].Food Chemistry,1995,53(3):285-293.
• [25]陈志军,李向红,刘永乐,等.鲢鱼蛋白酶法水解产物的功能性质[J].食品科学,2012,33(5):62-65.